New isoforms of rat Aquaporin-4.

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

New isoforms of rat Aquaporin-4. / Moe, Svein Erik; Sorbo, Jan Gunnar; Søgaard, Rikke; Zeuthen, Thomas; Petter Ottersen, Ole; Holen, Torgeir.

In: Genomics, Vol. 91, No. 4, 2008, p. 367-77.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Moe, SE, Sorbo, JG, Søgaard, R, Zeuthen, T, Petter Ottersen, O & Holen, T 2008, 'New isoforms of rat Aquaporin-4.', Genomics, vol. 91, no. 4, pp. 367-77. https://doi.org/10.1016/j.ygeno.2007.12.003

APA

Moe, S. E., Sorbo, J. G., Søgaard, R., Zeuthen, T., Petter Ottersen, O., & Holen, T. (2008). New isoforms of rat Aquaporin-4. Genomics, 91(4), 367-77. https://doi.org/10.1016/j.ygeno.2007.12.003

Vancouver

Moe SE, Sorbo JG, Søgaard R, Zeuthen T, Petter Ottersen O, Holen T. New isoforms of rat Aquaporin-4. Genomics. 2008;91(4):367-77. https://doi.org/10.1016/j.ygeno.2007.12.003

Author

Moe, Svein Erik ; Sorbo, Jan Gunnar ; Søgaard, Rikke ; Zeuthen, Thomas ; Petter Ottersen, Ole ; Holen, Torgeir. / New isoforms of rat Aquaporin-4. In: Genomics. 2008 ; Vol. 91, No. 4. pp. 367-77.

Bibtex

@article{a42abd6084be11dd81b0000ea68e967b,
title = "New isoforms of rat Aquaporin-4.",
abstract = "Aquaporin-4 (AQP4) is a brain aquaporin implicated in the pathophysiology of numerous clinical conditions including brain edema. Here we show that rat AQP4 has six cDNA isoforms, formed by alternative splicing. These are named AQP4a-f, where AQP4a and AQP4c correspond to the two classical M1 and M23 isoforms, respectively. The various isoforms are differentially expressed in kidney and brain, and their prevalence does not correspond to the level of the respective mRNAs, pointing to posttranscriptional regulation. The three isoforms lacking exon 2, AQP4b, AQP4d, and AQP4f, have an intracellular localization when expressed in cell lines and do not transport water when expressed in Xenopus oocytes. In contrast, the largest of the new isoforms, AQP4e, which contains a novel N-terminal domain, is localized at the plasma membrane in cell lines and functions as a water transporter in Xenopus oocytes.",
author = "Moe, {Svein Erik} and Sorbo, {Jan Gunnar} and Rikke S{\o}gaard and Thomas Zeuthen and {Petter Ottersen}, Ole and Torgeir Holen",
note = "Keywords: Alternative Splicing; Animals; Aquaporin 4; Base Sequence; Cell Line; DNA Primers; DNA, Complementary; Hela Cells; Humans; Protein Isoforms; RNA, Messenger; Rats; Subcellular Fractions",
year = "2008",
doi = "10.1016/j.ygeno.2007.12.003",
language = "English",
volume = "91",
pages = "367--77",
journal = "Genomics",
issn = "0888-7543",
publisher = "Academic Press",
number = "4",

}

RIS

TY - JOUR

T1 - New isoforms of rat Aquaporin-4.

AU - Moe, Svein Erik

AU - Sorbo, Jan Gunnar

AU - Søgaard, Rikke

AU - Zeuthen, Thomas

AU - Petter Ottersen, Ole

AU - Holen, Torgeir

N1 - Keywords: Alternative Splicing; Animals; Aquaporin 4; Base Sequence; Cell Line; DNA Primers; DNA, Complementary; Hela Cells; Humans; Protein Isoforms; RNA, Messenger; Rats; Subcellular Fractions

PY - 2008

Y1 - 2008

N2 - Aquaporin-4 (AQP4) is a brain aquaporin implicated in the pathophysiology of numerous clinical conditions including brain edema. Here we show that rat AQP4 has six cDNA isoforms, formed by alternative splicing. These are named AQP4a-f, where AQP4a and AQP4c correspond to the two classical M1 and M23 isoforms, respectively. The various isoforms are differentially expressed in kidney and brain, and their prevalence does not correspond to the level of the respective mRNAs, pointing to posttranscriptional regulation. The three isoforms lacking exon 2, AQP4b, AQP4d, and AQP4f, have an intracellular localization when expressed in cell lines and do not transport water when expressed in Xenopus oocytes. In contrast, the largest of the new isoforms, AQP4e, which contains a novel N-terminal domain, is localized at the plasma membrane in cell lines and functions as a water transporter in Xenopus oocytes.

AB - Aquaporin-4 (AQP4) is a brain aquaporin implicated in the pathophysiology of numerous clinical conditions including brain edema. Here we show that rat AQP4 has six cDNA isoforms, formed by alternative splicing. These are named AQP4a-f, where AQP4a and AQP4c correspond to the two classical M1 and M23 isoforms, respectively. The various isoforms are differentially expressed in kidney and brain, and their prevalence does not correspond to the level of the respective mRNAs, pointing to posttranscriptional regulation. The three isoforms lacking exon 2, AQP4b, AQP4d, and AQP4f, have an intracellular localization when expressed in cell lines and do not transport water when expressed in Xenopus oocytes. In contrast, the largest of the new isoforms, AQP4e, which contains a novel N-terminal domain, is localized at the plasma membrane in cell lines and functions as a water transporter in Xenopus oocytes.

U2 - 10.1016/j.ygeno.2007.12.003

DO - 10.1016/j.ygeno.2007.12.003

M3 - Journal article

C2 - 18255256

VL - 91

SP - 367

EP - 377

JO - Genomics

JF - Genomics

SN - 0888-7543

IS - 4

ER -

ID: 6093415