Multimodal detection of dopamine by sniffer cells expressing genetically encoded fluorescent sensors

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Standard

Multimodal detection of dopamine by sniffer cells expressing genetically encoded fluorescent sensors. / Klein Herenbrink, Carmen; Støier, Jonatan Fullerton; Reith, William Dalseg; Dagra, Abeer; Gregorek, Miguel Alejandro Cuadrado; Cola, Reto B; Patriarchi, Tommaso; Li, Yulong; Tian, Lin; Gether, Ulrik; Herborg, Freja.

In: Communications Biology , Vol. 5, No. 1, 578, 2022.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Klein Herenbrink, C, Støier, JF, Reith, WD, Dagra, A, Gregorek, MAC, Cola, RB, Patriarchi, T, Li, Y, Tian, L, Gether, U & Herborg, F 2022, 'Multimodal detection of dopamine by sniffer cells expressing genetically encoded fluorescent sensors', Communications Biology , vol. 5, no. 1, 578. https://doi.org/10.1038/s42003-022-03488-5

APA

Klein Herenbrink, C., Støier, J. F., Reith, W. D., Dagra, A., Gregorek, M. A. C., Cola, R. B., Patriarchi, T., Li, Y., Tian, L., Gether, U., & Herborg, F. (2022). Multimodal detection of dopamine by sniffer cells expressing genetically encoded fluorescent sensors. Communications Biology , 5(1), [578]. https://doi.org/10.1038/s42003-022-03488-5

Vancouver

Klein Herenbrink C, Støier JF, Reith WD, Dagra A, Gregorek MAC, Cola RB et al. Multimodal detection of dopamine by sniffer cells expressing genetically encoded fluorescent sensors. Communications Biology . 2022;5(1). 578. https://doi.org/10.1038/s42003-022-03488-5

Author

Klein Herenbrink, Carmen ; Støier, Jonatan Fullerton ; Reith, William Dalseg ; Dagra, Abeer ; Gregorek, Miguel Alejandro Cuadrado ; Cola, Reto B ; Patriarchi, Tommaso ; Li, Yulong ; Tian, Lin ; Gether, Ulrik ; Herborg, Freja. / Multimodal detection of dopamine by sniffer cells expressing genetically encoded fluorescent sensors. In: Communications Biology . 2022 ; Vol. 5, No. 1.

Bibtex

@article{a9581d752e074804b78edf8bd09f1bee,
title = "Multimodal detection of dopamine by sniffer cells expressing genetically encoded fluorescent sensors",
abstract = "Dopamine supports locomotor control and higher brain functions such as motivation and learning. Consistently, dopaminergic dysfunction is involved in a spectrum of neurological and neuropsychiatric diseases. Detailed data on dopamine dynamics is needed to understand how dopamine signals translate into cellular and behavioral responses, and to uncover pathological disturbances in dopamine-related diseases. Genetically encoded fluorescent dopamine sensors have recently enabled unprecedented monitoring of dopamine dynamics in vivo. However, these sensors' utility for in vitro and ex vivo assays remains unexplored. Here, we present a blueprint for making dopamine sniffer cells for multimodal dopamine detection. We generated sniffer cell lines with inducible expression of seven different dopamine sensors and perform a head-to-head comparison of sensor properties to guide users in sensor selection. In proof-of-principle experiments, we apply the sniffer cells to record endogenous dopamine release from cultured neurons and striatal slices, and for determining tissue dopamine content. Furthermore, we use the sniffer cells to measure dopamine uptake and release via the dopamine transporter as a radiotracer free, high-throughput alternative to electrochemical- and radiotracer-based assays. Importantly, the sniffer cell framework can readily be applied to the growing list of genetically encoded fluorescent neurotransmitter sensors.",
keywords = "Corpus Striatum/metabolism, Dopamine/metabolism, Learning, Neurons/metabolism, Neurotransmitter Agents",
author = "{Klein Herenbrink}, Carmen and St{\o}ier, {Jonatan Fullerton} and Reith, {William Dalseg} and Abeer Dagra and Gregorek, {Miguel Alejandro Cuadrado} and Cola, {Reto B} and Tommaso Patriarchi and Yulong Li and Lin Tian and Ulrik Gether and Freja Herborg",
note = "{\textcopyright} 2022. The Author(s).",
year = "2022",
doi = "10.1038/s42003-022-03488-5",
language = "English",
volume = "5",
journal = "Communications Biology",
issn = "2399-3642",
publisher = "nature publishing group",
number = "1",

}

RIS

TY - JOUR

T1 - Multimodal detection of dopamine by sniffer cells expressing genetically encoded fluorescent sensors

AU - Klein Herenbrink, Carmen

AU - Støier, Jonatan Fullerton

AU - Reith, William Dalseg

AU - Dagra, Abeer

AU - Gregorek, Miguel Alejandro Cuadrado

AU - Cola, Reto B

AU - Patriarchi, Tommaso

AU - Li, Yulong

AU - Tian, Lin

AU - Gether, Ulrik

AU - Herborg, Freja

N1 - © 2022. The Author(s).

PY - 2022

Y1 - 2022

N2 - Dopamine supports locomotor control and higher brain functions such as motivation and learning. Consistently, dopaminergic dysfunction is involved in a spectrum of neurological and neuropsychiatric diseases. Detailed data on dopamine dynamics is needed to understand how dopamine signals translate into cellular and behavioral responses, and to uncover pathological disturbances in dopamine-related diseases. Genetically encoded fluorescent dopamine sensors have recently enabled unprecedented monitoring of dopamine dynamics in vivo. However, these sensors' utility for in vitro and ex vivo assays remains unexplored. Here, we present a blueprint for making dopamine sniffer cells for multimodal dopamine detection. We generated sniffer cell lines with inducible expression of seven different dopamine sensors and perform a head-to-head comparison of sensor properties to guide users in sensor selection. In proof-of-principle experiments, we apply the sniffer cells to record endogenous dopamine release from cultured neurons and striatal slices, and for determining tissue dopamine content. Furthermore, we use the sniffer cells to measure dopamine uptake and release via the dopamine transporter as a radiotracer free, high-throughput alternative to electrochemical- and radiotracer-based assays. Importantly, the sniffer cell framework can readily be applied to the growing list of genetically encoded fluorescent neurotransmitter sensors.

AB - Dopamine supports locomotor control and higher brain functions such as motivation and learning. Consistently, dopaminergic dysfunction is involved in a spectrum of neurological and neuropsychiatric diseases. Detailed data on dopamine dynamics is needed to understand how dopamine signals translate into cellular and behavioral responses, and to uncover pathological disturbances in dopamine-related diseases. Genetically encoded fluorescent dopamine sensors have recently enabled unprecedented monitoring of dopamine dynamics in vivo. However, these sensors' utility for in vitro and ex vivo assays remains unexplored. Here, we present a blueprint for making dopamine sniffer cells for multimodal dopamine detection. We generated sniffer cell lines with inducible expression of seven different dopamine sensors and perform a head-to-head comparison of sensor properties to guide users in sensor selection. In proof-of-principle experiments, we apply the sniffer cells to record endogenous dopamine release from cultured neurons and striatal slices, and for determining tissue dopamine content. Furthermore, we use the sniffer cells to measure dopamine uptake and release via the dopamine transporter as a radiotracer free, high-throughput alternative to electrochemical- and radiotracer-based assays. Importantly, the sniffer cell framework can readily be applied to the growing list of genetically encoded fluorescent neurotransmitter sensors.

KW - Corpus Striatum/metabolism

KW - Dopamine/metabolism

KW - Learning

KW - Neurons/metabolism

KW - Neurotransmitter Agents

U2 - 10.1038/s42003-022-03488-5

DO - 10.1038/s42003-022-03488-5

M3 - Journal article

C2 - 35689020

VL - 5

JO - Communications Biology

JF - Communications Biology

SN - 2399-3642

IS - 1

M1 - 578

ER -

ID: 310910134