Mitochondrial DNA G13708A variation and multiple sclerosis: Is there an association?

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Mitochondrial DNA G13708A variation and multiple sclerosis: Is there an association? / Andalib, S.; Talebi, M.; Sakhinia, E.; Farhoudi, M.; Sadeghi-Bazargani, H.; Emamhadi, M. R.; Masoodian, N.; Balaghi-Inalou, M.; Vafaee, M. S.; Gjedde, A.

In: Revue Neurologique, Vol. 173, No. 3, 2017, p. 164-168.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Andalib, S, Talebi, M, Sakhinia, E, Farhoudi, M, Sadeghi-Bazargani, H, Emamhadi, MR, Masoodian, N, Balaghi-Inalou, M, Vafaee, MS & Gjedde, A 2017, 'Mitochondrial DNA G13708A variation and multiple sclerosis: Is there an association?', Revue Neurologique, vol. 173, no. 3, pp. 164-168. https://doi.org/10.1016/j.neurol.2017.02.002

APA

Andalib, S., Talebi, M., Sakhinia, E., Farhoudi, M., Sadeghi-Bazargani, H., Emamhadi, M. R., Masoodian, N., Balaghi-Inalou, M., Vafaee, M. S., & Gjedde, A. (2017). Mitochondrial DNA G13708A variation and multiple sclerosis: Is there an association? Revue Neurologique, 173(3), 164-168. https://doi.org/10.1016/j.neurol.2017.02.002

Vancouver

Andalib S, Talebi M, Sakhinia E, Farhoudi M, Sadeghi-Bazargani H, Emamhadi MR et al. Mitochondrial DNA G13708A variation and multiple sclerosis: Is there an association? Revue Neurologique. 2017;173(3):164-168. https://doi.org/10.1016/j.neurol.2017.02.002

Author

Andalib, S. ; Talebi, M. ; Sakhinia, E. ; Farhoudi, M. ; Sadeghi-Bazargani, H. ; Emamhadi, M. R. ; Masoodian, N. ; Balaghi-Inalou, M. ; Vafaee, M. S. ; Gjedde, A. / Mitochondrial DNA G13708A variation and multiple sclerosis: Is there an association?. In: Revue Neurologique. 2017 ; Vol. 173, No. 3. pp. 164-168.

Bibtex

@article{2cefdde01efe44439bc044991eef7dcb,
title = "Mitochondrial DNA G13708A variation and multiple sclerosis: Is there an association?",
abstract = "BackgroundMultiple sclerosis (MS) is considered a pathogenetic enigma. Recently, efforts to implicate genetics in human susceptibility to MS have identified an important role of mitochondrial DNA (mtDNA). G13708A is a common mtDNA variation associated with MS in specific populations. This study tested the hypothesis that the mtDNA G13708A variation is associated with MS in an Iranian population.Materials and methodsBlood samples were collected from 100 MS patients and 100 unrelated healthy controls. DNA was extracted using a salting-out method, followed by polymerase chain reaction (PCR) amplification. For assessment of restriction fragment length polymorphism (RFLP), PCR products were restricted by restriction enzyme Mva I. Thereafter, the restriction products were assessed by means of an ultraviolet (UV) transilluminator following electrophoresis with 3% agarose gel. Accuracy of the genotyping procedure was assessed by direct sequencing.ResultsThe mtDNA G13708A variation was found in 17 cases (17%) and 19 controls (19%) (P = 0.7, OR: 0.8, 95% CI: 0.3–1.9).ConclusionThe findings of the present study fail to support the hypothesis that the G13708A mtDNA variation is associated with MS in the selected Iranian population.",
keywords = "Multiple sclerosis, Mitochondrial DNA, mtDNA variation, G13708A, Iranian population",
author = "S. Andalib and M. Talebi and E. Sakhinia and M. Farhoudi and H. Sadeghi-Bazargani and Emamhadi, {M. R.} and N. Masoodian and M. Balaghi-Inalou and Vafaee, {M. S.} and A. Gjedde",
year = "2017",
doi = "10.1016/j.neurol.2017.02.002",
language = "English",
volume = "173",
pages = "164--168",
journal = "Revue Neurologique",
issn = "0035-3787",
publisher = "Elsevier Masson",
number = "3",

}

RIS

TY - JOUR

T1 - Mitochondrial DNA G13708A variation and multiple sclerosis: Is there an association?

AU - Andalib, S.

AU - Talebi, M.

AU - Sakhinia, E.

AU - Farhoudi, M.

AU - Sadeghi-Bazargani, H.

AU - Emamhadi, M. R.

AU - Masoodian, N.

AU - Balaghi-Inalou, M.

AU - Vafaee, M. S.

AU - Gjedde, A.

PY - 2017

Y1 - 2017

N2 - BackgroundMultiple sclerosis (MS) is considered a pathogenetic enigma. Recently, efforts to implicate genetics in human susceptibility to MS have identified an important role of mitochondrial DNA (mtDNA). G13708A is a common mtDNA variation associated with MS in specific populations. This study tested the hypothesis that the mtDNA G13708A variation is associated with MS in an Iranian population.Materials and methodsBlood samples were collected from 100 MS patients and 100 unrelated healthy controls. DNA was extracted using a salting-out method, followed by polymerase chain reaction (PCR) amplification. For assessment of restriction fragment length polymorphism (RFLP), PCR products were restricted by restriction enzyme Mva I. Thereafter, the restriction products were assessed by means of an ultraviolet (UV) transilluminator following electrophoresis with 3% agarose gel. Accuracy of the genotyping procedure was assessed by direct sequencing.ResultsThe mtDNA G13708A variation was found in 17 cases (17%) and 19 controls (19%) (P = 0.7, OR: 0.8, 95% CI: 0.3–1.9).ConclusionThe findings of the present study fail to support the hypothesis that the G13708A mtDNA variation is associated with MS in the selected Iranian population.

AB - BackgroundMultiple sclerosis (MS) is considered a pathogenetic enigma. Recently, efforts to implicate genetics in human susceptibility to MS have identified an important role of mitochondrial DNA (mtDNA). G13708A is a common mtDNA variation associated with MS in specific populations. This study tested the hypothesis that the mtDNA G13708A variation is associated with MS in an Iranian population.Materials and methodsBlood samples were collected from 100 MS patients and 100 unrelated healthy controls. DNA was extracted using a salting-out method, followed by polymerase chain reaction (PCR) amplification. For assessment of restriction fragment length polymorphism (RFLP), PCR products were restricted by restriction enzyme Mva I. Thereafter, the restriction products were assessed by means of an ultraviolet (UV) transilluminator following electrophoresis with 3% agarose gel. Accuracy of the genotyping procedure was assessed by direct sequencing.ResultsThe mtDNA G13708A variation was found in 17 cases (17%) and 19 controls (19%) (P = 0.7, OR: 0.8, 95% CI: 0.3–1.9).ConclusionThe findings of the present study fail to support the hypothesis that the G13708A mtDNA variation is associated with MS in the selected Iranian population.

KW - Multiple sclerosis

KW - Mitochondrial DNA

KW - mtDNA variation

KW - G13708A

KW - Iranian population

U2 - 10.1016/j.neurol.2017.02.002

DO - 10.1016/j.neurol.2017.02.002

M3 - Journal article

C2 - 28341142

VL - 173

SP - 164

EP - 168

JO - Revue Neurologique

JF - Revue Neurologique

SN - 0035-3787

IS - 3

ER -

ID: 182544921