Localization of the Priming Factors CAPS1 and CAPS2 in Mouse Sensory Neurons Is Determined by Their N-Termini

Research output: Contribution to journal › Journal article › Research › peer-review

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Localization of the Priming Factors CAPS1 and CAPS2 in Mouse Sensory Neurons Is Determined by Their N-Termini. / Staudt, Angelina; Ratai, Olga; Bouzouina, Aicha; Fecher-Trost, Claudia; Shaaban, Ahmed; Bzeih, Hawraa; Horn, Alexander; Shaib, Ali H.; Klose, Margarete; Flockerzi, Veit; Lauterbach, Marcel A.; Rettig, Jens; Becherer, Ute.

In: Frontiers in Molecular Neuroscience, Vol. 15, 674243, 2022.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Staudt, A, Ratai, O, Bouzouina, A, Fecher-Trost, C, Shaaban, A, Bzeih, H, Horn, A, Shaib, AH, Klose, M, Flockerzi, V, Lauterbach, MA, Rettig, J & Becherer, U 2022, 'Localization of the Priming Factors CAPS1 and CAPS2 in Mouse Sensory Neurons Is Determined by Their N-Termini', Frontiers in Molecular Neuroscience, vol. 15, 674243. https://doi.org/10.3389/fnmol.2022.674243

APA

Staudt, A., Ratai, O., Bouzouina, A., Fecher-Trost, C., Shaaban, A., Bzeih, H., Horn, A., Shaib, A. H., Klose, M., Flockerzi, V., Lauterbach, M. A., Rettig, J., & Becherer, U. (2022). Localization of the Priming Factors CAPS1 and CAPS2 in Mouse Sensory Neurons Is Determined by Their N-Termini. Frontiers in Molecular Neuroscience, 15, [674243]. https://doi.org/10.3389/fnmol.2022.674243

Vancouver

Staudt A, Ratai O, Bouzouina A, Fecher-Trost C, Shaaban A, Bzeih H et al. Localization of the Priming Factors CAPS1 and CAPS2 in Mouse Sensory Neurons Is Determined by Their N-Termini. Frontiers in Molecular Neuroscience. 2022;15. 674243. https://doi.org/10.3389/fnmol.2022.674243

Author

Staudt, Angelina ; Ratai, Olga ; Bouzouina, Aicha ; Fecher-Trost, Claudia ; Shaaban, Ahmed ; Bzeih, Hawraa ; Horn, Alexander ; Shaib, Ali H. ; Klose, Margarete ; Flockerzi, Veit ; Lauterbach, Marcel A. ; Rettig, Jens ; Becherer, Ute. / Localization of the Priming Factors CAPS1 and CAPS2 in Mouse Sensory Neurons Is Determined by Their N-Termini. In: Frontiers in Molecular Neuroscience. 2022 ; Vol. 15.

Bibtex

@article{99125daaf2574edfa46ad4868439b852,
title = "Localization of the Priming Factors CAPS1 and CAPS2 in Mouse Sensory Neurons Is Determined by Their N-Termini",
abstract = "Both paralogs of the calcium-dependent activator protein for secretion (CAPS) are required for exocytosis of synaptic vesicles (SVs) and large dense core vesicles (LDCVs). Despite approximately 80% sequence identity, CAPS1 and CAPS2 have distinct functions in promoting exocytosis of SVs and LDCVs in dorsal root ganglion (DRG) neurons. However, the molecular mechanisms underlying these differences remain enigmatic. In this study, we applied high- and super-resolution imaging techniques to systematically assess the subcellular localization of CAPS paralogs in DRG neurons deficient in both CAPS1 and CAPS2. CAPS1 was found to be more enriched at the synapses. Using – in-depth sequence analysis, we identified a unique CAPS1 N-terminal sequence, which we introduced into CAPS2. This CAPS1/2 chimera reproduced the pre-synaptic localization of CAPS1 and partially rescued synaptic transmission in neurons devoid of CAPS1 and CAPS2. Using immunoprecipitation combined with mass spectrometry, we identified CAPS1-specific interaction partners that could be responsible for its pre-synaptic enrichment. Taken together, these data suggest an important role of the CAPS1-N terminus in the localization of the protein at pre-synapses.",
keywords = "active zone, cellular localization, dorsal root ganglion, FRAP, intracellular trafficking, STED microscopy, synapse",
author = "Angelina Staudt and Olga Ratai and Aicha Bouzouina and Claudia Fecher-Trost and Ahmed Shaaban and Hawraa Bzeih and Alexander Horn and Shaib, {Ali H.} and Margarete Klose and Veit Flockerzi and Lauterbach, {Marcel A.} and Jens Rettig and Ute Becherer",
note = "Publisher Copyright: Copyright {\textcopyright} 2022 Staudt, Ratai, Bouzouina, Fecher-Trost, Shaaban, Bzeih, Horn, Shaib, Klose, Flockerzi, Lauterbach, Rettig and Becherer.",
year = "2022",
doi = "10.3389/fnmol.2022.674243",
language = "English",
volume = "15",
journal = "Frontiers in Molecular Neuroscience",
issn = "1662-5099",
publisher = "Frontiers Research Foundation",

}

RIS

TY - JOUR

T1 - Localization of the Priming Factors CAPS1 and CAPS2 in Mouse Sensory Neurons Is Determined by Their N-Termini

AU - Staudt, Angelina

AU - Ratai, Olga

AU - Bouzouina, Aicha

AU - Fecher-Trost, Claudia

AU - Shaaban, Ahmed

AU - Bzeih, Hawraa

AU - Horn, Alexander

AU - Shaib, Ali H.

AU - Klose, Margarete

AU - Flockerzi, Veit

AU - Lauterbach, Marcel A.

AU - Rettig, Jens

AU - Becherer, Ute

N1 - Publisher Copyright: Copyright © 2022 Staudt, Ratai, Bouzouina, Fecher-Trost, Shaaban, Bzeih, Horn, Shaib, Klose, Flockerzi, Lauterbach, Rettig and Becherer.

PY - 2022

Y1 - 2022

N2 - Both paralogs of the calcium-dependent activator protein for secretion (CAPS) are required for exocytosis of synaptic vesicles (SVs) and large dense core vesicles (LDCVs). Despite approximately 80% sequence identity, CAPS1 and CAPS2 have distinct functions in promoting exocytosis of SVs and LDCVs in dorsal root ganglion (DRG) neurons. However, the molecular mechanisms underlying these differences remain enigmatic. In this study, we applied high- and super-resolution imaging techniques to systematically assess the subcellular localization of CAPS paralogs in DRG neurons deficient in both CAPS1 and CAPS2. CAPS1 was found to be more enriched at the synapses. Using – in-depth sequence analysis, we identified a unique CAPS1 N-terminal sequence, which we introduced into CAPS2. This CAPS1/2 chimera reproduced the pre-synaptic localization of CAPS1 and partially rescued synaptic transmission in neurons devoid of CAPS1 and CAPS2. Using immunoprecipitation combined with mass spectrometry, we identified CAPS1-specific interaction partners that could be responsible for its pre-synaptic enrichment. Taken together, these data suggest an important role of the CAPS1-N terminus in the localization of the protein at pre-synapses.

AB - Both paralogs of the calcium-dependent activator protein for secretion (CAPS) are required for exocytosis of synaptic vesicles (SVs) and large dense core vesicles (LDCVs). Despite approximately 80% sequence identity, CAPS1 and CAPS2 have distinct functions in promoting exocytosis of SVs and LDCVs in dorsal root ganglion (DRG) neurons. However, the molecular mechanisms underlying these differences remain enigmatic. In this study, we applied high- and super-resolution imaging techniques to systematically assess the subcellular localization of CAPS paralogs in DRG neurons deficient in both CAPS1 and CAPS2. CAPS1 was found to be more enriched at the synapses. Using – in-depth sequence analysis, we identified a unique CAPS1 N-terminal sequence, which we introduced into CAPS2. This CAPS1/2 chimera reproduced the pre-synaptic localization of CAPS1 and partially rescued synaptic transmission in neurons devoid of CAPS1 and CAPS2. Using immunoprecipitation combined with mass spectrometry, we identified CAPS1-specific interaction partners that could be responsible for its pre-synaptic enrichment. Taken together, these data suggest an important role of the CAPS1-N terminus in the localization of the protein at pre-synapses.

KW - active zone

KW - cellular localization

KW - dorsal root ganglion

KW - FRAP

KW - intracellular trafficking

KW - STED microscopy

KW - synapse

U2 - 10.3389/fnmol.2022.674243

DO - 10.3389/fnmol.2022.674243

M3 - Journal article

C2 - 35493323

AN - SCOPUS:85128945569

VL - 15

JO - Frontiers in Molecular Neuroscience

JF - Frontiers in Molecular Neuroscience

SN - 1662-5099

M1 - 674243

ER -

ID: 305917860