Local impermeant anions establish the neuronal chloride concentration

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Local impermeant anions establish the neuronal chloride concentration. / Glykys, J; Dzhala, V; Egawa, K; Balena, T; Saponjian, Y; Kuchibhotla, K V; Bacskai, B J; Kahle, K T; Zeuthen, T; Staley, K J.

In: Science (New York, N.Y.), Vol. 343, No. 6171, 07.02.2014, p. 670-5.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Glykys, J, Dzhala, V, Egawa, K, Balena, T, Saponjian, Y, Kuchibhotla, KV, Bacskai, BJ, Kahle, KT, Zeuthen, T & Staley, KJ 2014, 'Local impermeant anions establish the neuronal chloride concentration', Science (New York, N.Y.), vol. 343, no. 6171, pp. 670-5. https://doi.org/10.1126/science.1245423

APA

Glykys, J., Dzhala, V., Egawa, K., Balena, T., Saponjian, Y., Kuchibhotla, K. V., Bacskai, B. J., Kahle, K. T., Zeuthen, T., & Staley, K. J. (2014). Local impermeant anions establish the neuronal chloride concentration. Science (New York, N.Y.), 343(6171), 670-5. https://doi.org/10.1126/science.1245423

Vancouver

Glykys J, Dzhala V, Egawa K, Balena T, Saponjian Y, Kuchibhotla KV et al. Local impermeant anions establish the neuronal chloride concentration. Science (New York, N.Y.). 2014 Feb 7;343(6171):670-5. https://doi.org/10.1126/science.1245423

Author

Glykys, J ; Dzhala, V ; Egawa, K ; Balena, T ; Saponjian, Y ; Kuchibhotla, K V ; Bacskai, B J ; Kahle, K T ; Zeuthen, T ; Staley, K J. / Local impermeant anions establish the neuronal chloride concentration. In: Science (New York, N.Y.). 2014 ; Vol. 343, No. 6171. pp. 670-5.

Bibtex

@article{02f1c964f01249b4bc4c4b0870ddf5f7,
title = "Local impermeant anions establish the neuronal chloride concentration",
abstract = "Neuronal intracellular chloride concentration [Cl(-)](i) is an important determinant of γ-aminobutyric acid type A (GABA(A)) receptor (GABA(A)R)-mediated inhibition and cytoplasmic volume regulation. Equilibrative cation-chloride cotransporters (CCCs) move Cl(-) across the membrane, but accumulating evidence suggests factors other than the bulk concentrations of transported ions determine [Cl(-)](i). Measurement of [Cl(-)](i) in murine brain slice preparations expressing the transgenic fluorophore Clomeleon demonstrated that cytoplasmic impermeant anions ([A](i)) and polyanionic extracellular matrix glycoproteins ([A](o)) constrain the local [Cl(-)]. CCC inhibition had modest effects on [Cl(-)](i) and neuronal volume, but substantial changes were produced by alterations of the balance between [A](i) and [A](o). Therefore, CCCs are important elements of Cl(-) homeostasis, but local impermeant anions determine the homeostatic set point for [Cl(-)], and hence, neuronal volume and the polarity of local GABA(A)R signaling.",
keywords = "Animals, Brain, Cell Membrane Permeability, Cell Polarity, Chloride Channels, Chlorides, Cytoplasm, Extracellular Matrix Proteins, Glycoproteins, Mice, Mice, Transgenic, Neurons, Receptors, GABA-A, Recombinant Fusion Proteins, Signal Transduction",
author = "J Glykys and V Dzhala and K Egawa and T Balena and Y Saponjian and Kuchibhotla, {K V} and Bacskai, {B J} and Kahle, {K T} and T Zeuthen and Staley, {K J}",
year = "2014",
month = feb,
day = "7",
doi = "10.1126/science.1245423",
language = "English",
volume = "343",
pages = "670--5",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "6171",

}

RIS

TY - JOUR

T1 - Local impermeant anions establish the neuronal chloride concentration

AU - Glykys, J

AU - Dzhala, V

AU - Egawa, K

AU - Balena, T

AU - Saponjian, Y

AU - Kuchibhotla, K V

AU - Bacskai, B J

AU - Kahle, K T

AU - Zeuthen, T

AU - Staley, K J

PY - 2014/2/7

Y1 - 2014/2/7

N2 - Neuronal intracellular chloride concentration [Cl(-)](i) is an important determinant of γ-aminobutyric acid type A (GABA(A)) receptor (GABA(A)R)-mediated inhibition and cytoplasmic volume regulation. Equilibrative cation-chloride cotransporters (CCCs) move Cl(-) across the membrane, but accumulating evidence suggests factors other than the bulk concentrations of transported ions determine [Cl(-)](i). Measurement of [Cl(-)](i) in murine brain slice preparations expressing the transgenic fluorophore Clomeleon demonstrated that cytoplasmic impermeant anions ([A](i)) and polyanionic extracellular matrix glycoproteins ([A](o)) constrain the local [Cl(-)]. CCC inhibition had modest effects on [Cl(-)](i) and neuronal volume, but substantial changes were produced by alterations of the balance between [A](i) and [A](o). Therefore, CCCs are important elements of Cl(-) homeostasis, but local impermeant anions determine the homeostatic set point for [Cl(-)], and hence, neuronal volume and the polarity of local GABA(A)R signaling.

AB - Neuronal intracellular chloride concentration [Cl(-)](i) is an important determinant of γ-aminobutyric acid type A (GABA(A)) receptor (GABA(A)R)-mediated inhibition and cytoplasmic volume regulation. Equilibrative cation-chloride cotransporters (CCCs) move Cl(-) across the membrane, but accumulating evidence suggests factors other than the bulk concentrations of transported ions determine [Cl(-)](i). Measurement of [Cl(-)](i) in murine brain slice preparations expressing the transgenic fluorophore Clomeleon demonstrated that cytoplasmic impermeant anions ([A](i)) and polyanionic extracellular matrix glycoproteins ([A](o)) constrain the local [Cl(-)]. CCC inhibition had modest effects on [Cl(-)](i) and neuronal volume, but substantial changes were produced by alterations of the balance between [A](i) and [A](o). Therefore, CCCs are important elements of Cl(-) homeostasis, but local impermeant anions determine the homeostatic set point for [Cl(-)], and hence, neuronal volume and the polarity of local GABA(A)R signaling.

KW - Animals

KW - Brain

KW - Cell Membrane Permeability

KW - Cell Polarity

KW - Chloride Channels

KW - Chlorides

KW - Cytoplasm

KW - Extracellular Matrix Proteins

KW - Glycoproteins

KW - Mice

KW - Mice, Transgenic

KW - Neurons

KW - Receptors, GABA-A

KW - Recombinant Fusion Proteins

KW - Signal Transduction

U2 - 10.1126/science.1245423

DO - 10.1126/science.1245423

M3 - Journal article

C2 - 24503855

VL - 343

SP - 670

EP - 675

JO - Science

JF - Science

SN - 0036-8075

IS - 6171

ER -

ID: 109651930