Kir4.1-mediated spatial buffering of K(+): Experimental challenges in determination of its temporal and quantitative contribution to K(+) clearance in the brain

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Standard

Kir4.1-mediated spatial buffering of K(+) : Experimental challenges in determination of its temporal and quantitative contribution to K(+) clearance in the brain. / Larsen, Brian Roland; MacAulay, Nanna.

In: Channels (Austin), Vol. 8, No. 6, 10.2014, p. 544-550.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Larsen, BR & MacAulay, N 2014, 'Kir4.1-mediated spatial buffering of K(+): Experimental challenges in determination of its temporal and quantitative contribution to K(+) clearance in the brain', Channels (Austin), vol. 8, no. 6, pp. 544-550. https://doi.org/10.4161/19336950.2014.970448

APA

Larsen, B. R., & MacAulay, N. (2014). Kir4.1-mediated spatial buffering of K(+): Experimental challenges in determination of its temporal and quantitative contribution to K(+) clearance in the brain. Channels (Austin), 8(6), 544-550. https://doi.org/10.4161/19336950.2014.970448

Vancouver

Larsen BR, MacAulay N. Kir4.1-mediated spatial buffering of K(+): Experimental challenges in determination of its temporal and quantitative contribution to K(+) clearance in the brain. Channels (Austin). 2014 Oct;8(6):544-550. https://doi.org/10.4161/19336950.2014.970448

Author

Larsen, Brian Roland ; MacAulay, Nanna. / Kir4.1-mediated spatial buffering of K(+) : Experimental challenges in determination of its temporal and quantitative contribution to K(+) clearance in the brain. In: Channels (Austin). 2014 ; Vol. 8, No. 6. pp. 544-550.

Bibtex

@article{78d6fbe09d9e4d539d3f6bcb398fcb2f,
title = "Kir4.1-mediated spatial buffering of K(+): Experimental challenges in determination of its temporal and quantitative contribution to K(+) clearance in the brain",
abstract = "Abstract Neuronal activity results in release of K(+) into the extracellular space of the central nervous system. If the excess K(+) is allowed to accumulate, neuronal firing will be compromised by the ensuing neuronal membrane depolarization. The surrounding glial cells are involved in clearing K(+) from the extracellular space by molecular mechanism(s), the identity of which have been a matter of controversy for over half a century. Kir4.1-mediated spatial buffering of K(+) has been promoted as a major contributor to K(+) removal although its quantitative and temporal contribution has remained undefined. We discuss the biophysical and experimental challenges regarding determination of the contribution of Kir4.1 to extracellular K(+) management during neuronal activity. It is concluded that 1) the geometry of the experimental preparation is crucial for detection of Kir4.1-mediated spatial buffering and 2) Kir4.1 enacts spatial buffering of K(+) during but not after neuronal activity.",
author = "Larsen, {Brian Roland} and Nanna MacAulay",
year = "2014",
month = oct,
doi = "10.4161/19336950.2014.970448",
language = "English",
volume = "8",
pages = "544--550",
journal = "Channels",
issn = "1933-6950",
publisher = "Taylor & Francis",
number = "6",

}

RIS

TY - JOUR

T1 - Kir4.1-mediated spatial buffering of K(+)

T2 - Experimental challenges in determination of its temporal and quantitative contribution to K(+) clearance in the brain

AU - Larsen, Brian Roland

AU - MacAulay, Nanna

PY - 2014/10

Y1 - 2014/10

N2 - Abstract Neuronal activity results in release of K(+) into the extracellular space of the central nervous system. If the excess K(+) is allowed to accumulate, neuronal firing will be compromised by the ensuing neuronal membrane depolarization. The surrounding glial cells are involved in clearing K(+) from the extracellular space by molecular mechanism(s), the identity of which have been a matter of controversy for over half a century. Kir4.1-mediated spatial buffering of K(+) has been promoted as a major contributor to K(+) removal although its quantitative and temporal contribution has remained undefined. We discuss the biophysical and experimental challenges regarding determination of the contribution of Kir4.1 to extracellular K(+) management during neuronal activity. It is concluded that 1) the geometry of the experimental preparation is crucial for detection of Kir4.1-mediated spatial buffering and 2) Kir4.1 enacts spatial buffering of K(+) during but not after neuronal activity.

AB - Abstract Neuronal activity results in release of K(+) into the extracellular space of the central nervous system. If the excess K(+) is allowed to accumulate, neuronal firing will be compromised by the ensuing neuronal membrane depolarization. The surrounding glial cells are involved in clearing K(+) from the extracellular space by molecular mechanism(s), the identity of which have been a matter of controversy for over half a century. Kir4.1-mediated spatial buffering of K(+) has been promoted as a major contributor to K(+) removal although its quantitative and temporal contribution has remained undefined. We discuss the biophysical and experimental challenges regarding determination of the contribution of Kir4.1 to extracellular K(+) management during neuronal activity. It is concluded that 1) the geometry of the experimental preparation is crucial for detection of Kir4.1-mediated spatial buffering and 2) Kir4.1 enacts spatial buffering of K(+) during but not after neuronal activity.

U2 - 10.4161/19336950.2014.970448

DO - 10.4161/19336950.2014.970448

M3 - Journal article

C2 - 25483287

VL - 8

SP - 544

EP - 550

JO - Channels

JF - Channels

SN - 1933-6950

IS - 6

ER -

ID: 129810148