Inhibition of hippocampal synaptic transmission by impairment of Ral function.

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Inhibition of hippocampal synaptic transmission by impairment of Ral function. / Owe-Larsson, Björn; Chaves-Olarte, Esteban; Chauhan, Ashok; Kjaerulff, Ole; Brask, Johan; Thelestam, Monica; Brodin, Lennart; Löw, Peter.

In: NeuroReport, Vol. 16, No. 16, 2005, p. 1805-8.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Owe-Larsson, B, Chaves-Olarte, E, Chauhan, A, Kjaerulff, O, Brask, J, Thelestam, M, Brodin, L & Löw, P 2005, 'Inhibition of hippocampal synaptic transmission by impairment of Ral function.', NeuroReport, vol. 16, no. 16, pp. 1805-8.

APA

Owe-Larsson, B., Chaves-Olarte, E., Chauhan, A., Kjaerulff, O., Brask, J., Thelestam, M., Brodin, L., & Löw, P. (2005). Inhibition of hippocampal synaptic transmission by impairment of Ral function. NeuroReport, 16(16), 1805-8.

Vancouver

Owe-Larsson B, Chaves-Olarte E, Chauhan A, Kjaerulff O, Brask J, Thelestam M et al. Inhibition of hippocampal synaptic transmission by impairment of Ral function. NeuroReport. 2005;16(16):1805-8.

Author

Owe-Larsson, Björn ; Chaves-Olarte, Esteban ; Chauhan, Ashok ; Kjaerulff, Ole ; Brask, Johan ; Thelestam, Monica ; Brodin, Lennart ; Löw, Peter. / Inhibition of hippocampal synaptic transmission by impairment of Ral function. In: NeuroReport. 2005 ; Vol. 16, No. 16. pp. 1805-8.

Bibtex

@article{41a54c00752311dd8d9f000ea68e967b,
title = "Inhibition of hippocampal synaptic transmission by impairment of Ral function.",
abstract = "Large clostridial cytotoxins and protein overexpression were used to probe for involvement of Ras-related GTPases (guanosine triphosphate) in synaptic transmission in cultured rat hippocampal neurons. The toxins TcdA-10463 (inactivates Rho, Rac, Cdc42, Rap) and TcsL-1522 (inactivates Ral, Rac, Ras, R-Ras, Rap) both inhibited autaptic responses. In a proportion of the neurons (25%, TcdA-10463; 54%, TcsL-1522), the inhibition was associated with a shift from activity-dependent depression to facilitation, indicating that the synaptic release probability was reduced. Overexpression of a dominant negative Ral mutant, Ral A28N, caused a strong inhibition of autaptic responses, which was associated with a shift to facilitation in a majority (80%) of the neurons. These results indicate that Ral, along with at least one other non-Rab GTPase, participates in presynaptic regulation in hippocampal neurons.",
author = "Bj{\"o}rn Owe-Larsson and Esteban Chaves-Olarte and Ashok Chauhan and Ole Kjaerulff and Johan Brask and Monica Thelestam and Lennart Brodin and Peter L{\"o}w",
note = "Keywords: Alanine; Animals; Arabidopsis Proteins; Asparagine; Bacterial Toxins; Blotting, Western; Carbon Isotopes; Cells, Cultured; Drug Interactions; Electric Stimulation; Enterotoxins; Excitatory Amino Acid Agonists; Gene Expression Regulation; Glucose; Hippocampus; Membrane Potentials; Mutation; Neural Inhibition; Neurons; Patch-Clamp Techniques; Rats; Synaptic Transmission; Toxins, Biological; Transfection; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; gamma-Aminobutyric Acid; ral GTP-Binding Proteins",
year = "2005",
language = "English",
volume = "16",
pages = "1805--8",
journal = "NeuroReport",
issn = "0959-4965",
publisher = "Lippincott Williams & Wilkins",
number = "16",

}

RIS

TY - JOUR

T1 - Inhibition of hippocampal synaptic transmission by impairment of Ral function.

AU - Owe-Larsson, Björn

AU - Chaves-Olarte, Esteban

AU - Chauhan, Ashok

AU - Kjaerulff, Ole

AU - Brask, Johan

AU - Thelestam, Monica

AU - Brodin, Lennart

AU - Löw, Peter

N1 - Keywords: Alanine; Animals; Arabidopsis Proteins; Asparagine; Bacterial Toxins; Blotting, Western; Carbon Isotopes; Cells, Cultured; Drug Interactions; Electric Stimulation; Enterotoxins; Excitatory Amino Acid Agonists; Gene Expression Regulation; Glucose; Hippocampus; Membrane Potentials; Mutation; Neural Inhibition; Neurons; Patch-Clamp Techniques; Rats; Synaptic Transmission; Toxins, Biological; Transfection; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; gamma-Aminobutyric Acid; ral GTP-Binding Proteins

PY - 2005

Y1 - 2005

N2 - Large clostridial cytotoxins and protein overexpression were used to probe for involvement of Ras-related GTPases (guanosine triphosphate) in synaptic transmission in cultured rat hippocampal neurons. The toxins TcdA-10463 (inactivates Rho, Rac, Cdc42, Rap) and TcsL-1522 (inactivates Ral, Rac, Ras, R-Ras, Rap) both inhibited autaptic responses. In a proportion of the neurons (25%, TcdA-10463; 54%, TcsL-1522), the inhibition was associated with a shift from activity-dependent depression to facilitation, indicating that the synaptic release probability was reduced. Overexpression of a dominant negative Ral mutant, Ral A28N, caused a strong inhibition of autaptic responses, which was associated with a shift to facilitation in a majority (80%) of the neurons. These results indicate that Ral, along with at least one other non-Rab GTPase, participates in presynaptic regulation in hippocampal neurons.

AB - Large clostridial cytotoxins and protein overexpression were used to probe for involvement of Ras-related GTPases (guanosine triphosphate) in synaptic transmission in cultured rat hippocampal neurons. The toxins TcdA-10463 (inactivates Rho, Rac, Cdc42, Rap) and TcsL-1522 (inactivates Ral, Rac, Ras, R-Ras, Rap) both inhibited autaptic responses. In a proportion of the neurons (25%, TcdA-10463; 54%, TcsL-1522), the inhibition was associated with a shift from activity-dependent depression to facilitation, indicating that the synaptic release probability was reduced. Overexpression of a dominant negative Ral mutant, Ral A28N, caused a strong inhibition of autaptic responses, which was associated with a shift to facilitation in a majority (80%) of the neurons. These results indicate that Ral, along with at least one other non-Rab GTPase, participates in presynaptic regulation in hippocampal neurons.

M3 - Journal article

C2 - 16237331

VL - 16

SP - 1805

EP - 1808

JO - NeuroReport

JF - NeuroReport

SN - 0959-4965

IS - 16

ER -

ID: 5750214