In vivo amphetamine action is contingent on αCaMKII
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In vivo amphetamine action is contingent on αCaMKII. / Steinkellner, Thomas; Mus, Liudmilla; Eisenrauch, Birgit; Constantinescu, Andreea; Leo, Damiana; Konrad, Lisa; Rickhag, Karl Mattias; Sørensen, Gunnar; Efimova, Evgenia V; Kong, Eryan; Willeit, Matthäus; Sotnikova, Tatyana D; Kudlacek, Oliver; Gether, Ulrik; Freissmuth, Michael; Pollak, Daniela D; Gainetdinov, Raul R; Sitte, Harald H.
In: Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, Vol. 39, No. 11, 10.2014, p. 2681-93.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - In vivo amphetamine action is contingent on αCaMKII
AU - Steinkellner, Thomas
AU - Mus, Liudmilla
AU - Eisenrauch, Birgit
AU - Constantinescu, Andreea
AU - Leo, Damiana
AU - Konrad, Lisa
AU - Rickhag, Karl Mattias
AU - Sørensen, Gunnar
AU - Efimova, Evgenia V
AU - Kong, Eryan
AU - Willeit, Matthäus
AU - Sotnikova, Tatyana D
AU - Kudlacek, Oliver
AU - Gether, Ulrik
AU - Freissmuth, Michael
AU - Pollak, Daniela D
AU - Gainetdinov, Raul R
AU - Sitte, Harald H
PY - 2014/10
Y1 - 2014/10
N2 - Addiction to psychostimulants (ie, amphetamines and cocaine) imposes a major socioeconomic burden. Prevention and treatment represent unmet medical needs, which may be addressed, if the mechanisms underlying psychostimulant action are understood. Cocaine acts as a blocker at the transporters for dopamine (DAT), serotonin (SERT), and norepinephrine (NET), but amphetamines are substrates that do not only block the uptake of monoamines but also induce substrate efflux by promoting reverse transport. Reverse transport has been a focus of research for decades but its mechanistic basis still remains enigmatic. Recently, transporter-interacting proteins were found to regulate amphetamine-triggered reverse transport: calmodulin kinase IIα (αCaMKII) is a prominent example, because it binds the carboxyl terminus of DAT, phosphorylates its amino terminus, and supports amphetamine-induced substrate efflux in vitro. Here, we investigated whether, in vivo, the action of amphetamine was contingent on the presence of αCaMKII by recording the behavioral and neurochemical effects of amphetamine. Measurement of dopamine efflux in the dorsal striatum by microdialysis revealed that amphetamine induced less dopamine efflux in mice lacking αCaMKII. Consistent with this observation, the acute locomotor responses to amphetamine were also significantly blunted in αCaMKII-deficient mice. In addition, while the rewarding properties of amphetamine were preserved in αCaMKII-deficient mice, their behavioral sensitization to amphetamine was markedly reduced. Our findings demonstrate that amphetamine requires the presence of αCaMKII to elicit a full-fledged effect on DAT in vivo: αCaMKII does not only support acute amphetamine-induced dopamine efflux but is also important in shaping the chronic response to amphetamine.
AB - Addiction to psychostimulants (ie, amphetamines and cocaine) imposes a major socioeconomic burden. Prevention and treatment represent unmet medical needs, which may be addressed, if the mechanisms underlying psychostimulant action are understood. Cocaine acts as a blocker at the transporters for dopamine (DAT), serotonin (SERT), and norepinephrine (NET), but amphetamines are substrates that do not only block the uptake of monoamines but also induce substrate efflux by promoting reverse transport. Reverse transport has been a focus of research for decades but its mechanistic basis still remains enigmatic. Recently, transporter-interacting proteins were found to regulate amphetamine-triggered reverse transport: calmodulin kinase IIα (αCaMKII) is a prominent example, because it binds the carboxyl terminus of DAT, phosphorylates its amino terminus, and supports amphetamine-induced substrate efflux in vitro. Here, we investigated whether, in vivo, the action of amphetamine was contingent on the presence of αCaMKII by recording the behavioral and neurochemical effects of amphetamine. Measurement of dopamine efflux in the dorsal striatum by microdialysis revealed that amphetamine induced less dopamine efflux in mice lacking αCaMKII. Consistent with this observation, the acute locomotor responses to amphetamine were also significantly blunted in αCaMKII-deficient mice. In addition, while the rewarding properties of amphetamine were preserved in αCaMKII-deficient mice, their behavioral sensitization to amphetamine was markedly reduced. Our findings demonstrate that amphetamine requires the presence of αCaMKII to elicit a full-fledged effect on DAT in vivo: αCaMKII does not only support acute amphetamine-induced dopamine efflux but is also important in shaping the chronic response to amphetamine.
U2 - 10.1038/npp.2014.124
DO - 10.1038/npp.2014.124
M3 - Journal article
C2 - 24871545
VL - 39
SP - 2681
EP - 2693
JO - Neuropsychopharmacology
JF - Neuropsychopharmacology
SN - 0893-133X
IS - 11
ER -
ID: 132931179