In vitro generation of motor neuron precursors from mouse embryonic stem cells using mesoporous nanoparticles
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
In vitro generation of motor neuron precursors from mouse embryonic stem cells using mesoporous nanoparticles. / Garcia-Bennett, Alfonso E; König, Niclas; Abrahamsson, Ninnie; Kozhevnikova, Mariya; Zhou, Chunfang; Trolle, Carl; Pankratova, Stanislava; Berezin, Vladimir; Kozlova, Elena N.
In: Nanomedicine, Vol. 9, No. 16, 24.03.2014, p. 2457-66.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - In vitro generation of motor neuron precursors from mouse embryonic stem cells using mesoporous nanoparticles
AU - Garcia-Bennett, Alfonso E
AU - König, Niclas
AU - Abrahamsson, Ninnie
AU - Kozhevnikova, Mariya
AU - Zhou, Chunfang
AU - Trolle, Carl
AU - Pankratova, Stanislava
AU - Berezin, Vladimir
AU - Kozlova, Elena N
PY - 2014/3/24
Y1 - 2014/3/24
N2 - Aim: Stem cell-derived motor neurons (MNs) are utilized to develop replacement strategies for spinal cord disorders. Differentiation of embryonic stem cells into MN precursors involves factors and their repeated administration. We investigated if delivery of factors loaded into mesoporous nanoparticles could be effective for stem cell differentiation in vitro. Materials & methods: We used a mouse embryonic stem cell line expressing green fluorescent protein under the promoter for the MN-specific gene Hb9 to visualize the level of MN differentiation. The differentiation of stem cells was evaluated by expression of MN-specific transcription factors monitored by quantitative real-time PCR reactions and immunocytochemistry. Results: Mesoporous nanoparticles have strong affiliation to the embryoid bodies, penetrate inside the embryoid bodies and come in contact with differentiating cells. Conclusion: Repeated administration of soluble factors into a culture medium can be avoided due to a sustained release effect using mesoporous silica. Original submitted 28 August 2013; Revised submitted 23 January 2014.
AB - Aim: Stem cell-derived motor neurons (MNs) are utilized to develop replacement strategies for spinal cord disorders. Differentiation of embryonic stem cells into MN precursors involves factors and their repeated administration. We investigated if delivery of factors loaded into mesoporous nanoparticles could be effective for stem cell differentiation in vitro. Materials & methods: We used a mouse embryonic stem cell line expressing green fluorescent protein under the promoter for the MN-specific gene Hb9 to visualize the level of MN differentiation. The differentiation of stem cells was evaluated by expression of MN-specific transcription factors monitored by quantitative real-time PCR reactions and immunocytochemistry. Results: Mesoporous nanoparticles have strong affiliation to the embryoid bodies, penetrate inside the embryoid bodies and come in contact with differentiating cells. Conclusion: Repeated administration of soluble factors into a culture medium can be avoided due to a sustained release effect using mesoporous silica. Original submitted 28 August 2013; Revised submitted 23 January 2014.
U2 - 10.2217/nnm.14.23
DO - 10.2217/nnm.14.23
M3 - Journal article
C2 - 24661257
VL - 9
SP - 2457
EP - 2466
JO - Nanomedicine
JF - Nanomedicine
SN - 1743-5889
IS - 16
ER -
ID: 118949055