TY - JOUR

T1 - Identification of chromatophore membrane protein complexes formed under different nitrogen availability conditions in Rhodospirillum rubrum

AU - Selao, Tiago Toscano

AU - Branca, Rui

AU - Chae, Pil Seok

AU - Lehtiö, Janne

AU - Gellman, Samuel H

AU - Rasmussen, Søren Gøgsig Faarup

AU - Nordlund, Stefan

AU - Norén, Agneta

PY - 2011/6/3

Y1 - 2011/6/3

N2 - The chromatophore membrane of the photosynthetic diazotroph Rhodospirillum rubrum is of vital importance for a number of central processes, including nitrogen fixation. Using a novel amphiphile, we have identified protein complexes present under different nitrogen availability conditions by the use of two-dimensional Blue Native/SDS-PAGE and NSI-LC-LTQ-Orbitrap mass spectrometry. We have identified several membrane protein complexes, including components of the ATP synthase, reaction center, light harvesting, and NADH dehydrogenase complexes. Additionally, we have identified differentially expressed proteins, such as subunits of the succinate dehydrogenase complex and other TCA cycle enzymes that are usually found in the cytosol, thus hinting at a possible association to the membrane in response to nitrogen deficiency. We propose a redox sensing mechanism that can influence the membrane subproteome in response to nitrogen availability.

AB - The chromatophore membrane of the photosynthetic diazotroph Rhodospirillum rubrum is of vital importance for a number of central processes, including nitrogen fixation. Using a novel amphiphile, we have identified protein complexes present under different nitrogen availability conditions by the use of two-dimensional Blue Native/SDS-PAGE and NSI-LC-LTQ-Orbitrap mass spectrometry. We have identified several membrane protein complexes, including components of the ATP synthase, reaction center, light harvesting, and NADH dehydrogenase complexes. Additionally, we have identified differentially expressed proteins, such as subunits of the succinate dehydrogenase complex and other TCA cycle enzymes that are usually found in the cytosol, thus hinting at a possible association to the membrane in response to nitrogen deficiency. We propose a redox sensing mechanism that can influence the membrane subproteome in response to nitrogen availability.

KW - ATP Synthetase Complexes

KW - Ammonium Chloride

KW - Bacterial Chromatophores

KW - Citric Acid Cycle

KW - Electron Transport Complex I

KW - Electrophoresis, Gel, Two-Dimensional

KW - Flavoproteins

KW - Light-Harvesting Protein Complexes

KW - Membrane Proteins

KW - Membrane Transport Proteins

KW - Nitrogen

KW - Nitrogen Fixation

KW - Rhodospirillum rubrum

KW - Subcellular Fractions

U2 - 10.1021/pr100838x

DO - 10.1021/pr100838x

M3 - Journal article

C2 - 21443180

VL - 10

SP - 2703

EP - 2714

JO - Journal of Proteome Research

JF - Journal of Proteome Research

SN - 1535-3893

IS - 6

ER -