Fictive locomotion in the adult decerebrate and spinal mouse in vivo

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Fictive locomotion in the adult decerebrate and spinal mouse in vivo. / Meehan, Claire Francesca; Grøndahl, Lillian; Nielsen, Jens Bo; Hultborn, Hans R.

In: Journal of Physiology, Vol. 590, No. 2, 2012, p. 289-300.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Meehan, CF, Grøndahl, L, Nielsen, JB & Hultborn, HR 2012, 'Fictive locomotion in the adult decerebrate and spinal mouse in vivo', Journal of Physiology, vol. 590, no. 2, pp. 289-300. https://doi.org/10.1113/jphysiol.2011.214643

APA

Meehan, C. F., Grøndahl, L., Nielsen, J. B., & Hultborn, H. R. (2012). Fictive locomotion in the adult decerebrate and spinal mouse in vivo. Journal of Physiology, 590(2), 289-300. https://doi.org/10.1113/jphysiol.2011.214643

Vancouver

Meehan CF, Grøndahl L, Nielsen JB, Hultborn HR. Fictive locomotion in the adult decerebrate and spinal mouse in vivo. Journal of Physiology. 2012;590(2):289-300. https://doi.org/10.1113/jphysiol.2011.214643

Author

Meehan, Claire Francesca ; Grøndahl, Lillian ; Nielsen, Jens Bo ; Hultborn, Hans R. / Fictive locomotion in the adult decerebrate and spinal mouse in vivo. In: Journal of Physiology. 2012 ; Vol. 590, No. 2. pp. 289-300.

Bibtex

@article{5dd48f72cee5412fb9bf083a399d73c5,
title = "Fictive locomotion in the adult decerebrate and spinal mouse in vivo",
abstract = "Recently, transgenic mice have been created with mutations affecting the components of the mammalian spinal central pattern generator (CPG) for locomotion, however, it has currently only been possible to evoke fictive locomotion in mice, using neonatal in vitro preparations. Here, we demonstrate that it is possible to evoke fictive locomotion in the adult decerebrate mouse in vivo using L-3,4-Dihydroxyphenylalanine methyl ester hydrochloride (L-DOPA) and 5-hydroxytryptophan (5HTP) following injection of the monoaminoxiadase inhibitor Nialamide. We investigate the effects of afferent stimulation and spinalization as well as demonstrate the possibility of simultaneous intracellular recording of rhythmically active motoneurones. Our results demonstrate that several features of the mouse locomotor CPG are similar to that which have been observed in rat, cat, rabbit and monkey suggesting a fairly conserved organisation and allowing for future results in transgenic mice to be extrapolated to existing knowledge of CPG components and circuitry obtained in larger species.",
author = "Meehan, {Claire Francesca} and Lillian Gr{\o}ndahl and Nielsen, {Jens Bo} and Hultborn, {Hans R}",
note = "CURIS 2012 5200 007",
year = "2012",
doi = "10.1113/jphysiol.2011.214643",
language = "English",
volume = "590",
pages = "289--300",
journal = "The Journal of Physiology",
issn = "0022-3751",
publisher = "Wiley-Blackwell",
number = "2",

}

RIS

TY - JOUR

T1 - Fictive locomotion in the adult decerebrate and spinal mouse in vivo

AU - Meehan, Claire Francesca

AU - Grøndahl, Lillian

AU - Nielsen, Jens Bo

AU - Hultborn, Hans R

N1 - CURIS 2012 5200 007

PY - 2012

Y1 - 2012

N2 - Recently, transgenic mice have been created with mutations affecting the components of the mammalian spinal central pattern generator (CPG) for locomotion, however, it has currently only been possible to evoke fictive locomotion in mice, using neonatal in vitro preparations. Here, we demonstrate that it is possible to evoke fictive locomotion in the adult decerebrate mouse in vivo using L-3,4-Dihydroxyphenylalanine methyl ester hydrochloride (L-DOPA) and 5-hydroxytryptophan (5HTP) following injection of the monoaminoxiadase inhibitor Nialamide. We investigate the effects of afferent stimulation and spinalization as well as demonstrate the possibility of simultaneous intracellular recording of rhythmically active motoneurones. Our results demonstrate that several features of the mouse locomotor CPG are similar to that which have been observed in rat, cat, rabbit and monkey suggesting a fairly conserved organisation and allowing for future results in transgenic mice to be extrapolated to existing knowledge of CPG components and circuitry obtained in larger species.

AB - Recently, transgenic mice have been created with mutations affecting the components of the mammalian spinal central pattern generator (CPG) for locomotion, however, it has currently only been possible to evoke fictive locomotion in mice, using neonatal in vitro preparations. Here, we demonstrate that it is possible to evoke fictive locomotion in the adult decerebrate mouse in vivo using L-3,4-Dihydroxyphenylalanine methyl ester hydrochloride (L-DOPA) and 5-hydroxytryptophan (5HTP) following injection of the monoaminoxiadase inhibitor Nialamide. We investigate the effects of afferent stimulation and spinalization as well as demonstrate the possibility of simultaneous intracellular recording of rhythmically active motoneurones. Our results demonstrate that several features of the mouse locomotor CPG are similar to that which have been observed in rat, cat, rabbit and monkey suggesting a fairly conserved organisation and allowing for future results in transgenic mice to be extrapolated to existing knowledge of CPG components and circuitry obtained in larger species.

U2 - 10.1113/jphysiol.2011.214643

DO - 10.1113/jphysiol.2011.214643

M3 - Journal article

C2 - 22106172

VL - 590

SP - 289

EP - 300

JO - The Journal of Physiology

JF - The Journal of Physiology

SN - 0022-3751

IS - 2

ER -

ID: 36076088