Facilitation of plateau potentials in turtle motoneurones by a pathway dependent on calcium and calmodulin

Research output: Contribution to journal › Journal article › Research › peer-review

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Facilitation of plateau potentials in turtle motoneurones by a pathway dependent on calcium and calmodulin. / Perrier, J F; Mejia-Gervacio, S; Hounsgaard, J.

In: Journal of Physiology, Vol. 528 Pt 1, 01.10.2000, p. 107-13.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Perrier, JF, Mejia-Gervacio, S & Hounsgaard, J 2000, 'Facilitation of plateau potentials in turtle motoneurones by a pathway dependent on calcium and calmodulin', Journal of Physiology, vol. 528 Pt 1, pp. 107-13.

APA

Perrier, J. F., Mejia-Gervacio, S., & Hounsgaard, J. (2000). Facilitation of plateau potentials in turtle motoneurones by a pathway dependent on calcium and calmodulin. Journal of Physiology, 528 Pt 1, 107-13.

Vancouver

Perrier JF, Mejia-Gervacio S, Hounsgaard J. Facilitation of plateau potentials in turtle motoneurones by a pathway dependent on calcium and calmodulin. Journal of Physiology. 2000 Oct 1;528 Pt 1:107-13.

Author

Perrier, J F ; Mejia-Gervacio, S ; Hounsgaard, J. / Facilitation of plateau potentials in turtle motoneurones by a pathway dependent on calcium and calmodulin. In: Journal of Physiology. 2000 ; Vol. 528 Pt 1. pp. 107-13.

Bibtex

@article{338e948d01aa46d493bcdc428337f282,
title = "Facilitation of plateau potentials in turtle motoneurones by a pathway dependent on calcium and calmodulin",
abstract = "1. The involvement of intracellular calcium and calmodulin in the modulation of plateau potentials in motoneurones was investigated using intracellular recordings from a spinal cord slice preparation. 2. Chelation of intracellular calcium with BAPTA-AM or inactivation of calmodulin with W-7 or trifluoperazine reduced the amplitude of depolarization-induced plateau potentials. Inactivation of calmodulin also inhibited facilitation of plateau potentials by activation of group I metabotropic glutamate receptors or muscarinic receptors. 3. In low-sodium medium and in the presence of tetraethylammonium and tetrodotoxin, calcium action potentials evoked by depolarization were followed by a short hyperpolarization ascribed to the calcium-activated non-selective cationic current (ICAN) and by a dihydropyridine-sensitive afterdepolarization. The amplitude of the afterdepolarization depended on the number of calcium spikes and was mediated by L-type calcium channels. 4. The dihydropyridine-sensitive afterdepolarization induced by calcium spikes was reduced by blockade of calmodulin. 5. It is proposed that plateau potentials in spinal motoneurones are facilitated by activation of a calcium-calmodulin-dependent pathway.",
keywords = "Animals, Calcium, Calcium Channels, L-Type, Calmodulin, Dopamine Antagonists, Egtazic Acid, Intracellular Fluid, Membrane Potentials, Motor Neurons, Receptors, Metabotropic Glutamate, Receptors, Muscarinic, Sodium, Spinal Cord, Tetraethylammonium, Tetrodotoxin, Trifluoperazine, Turtles",
author = "Perrier, {J F} and S Mejia-Gervacio and J Hounsgaard",
year = "2000",
month = oct,
day = "1",
language = "English",
volume = "528 Pt 1",
pages = "107--13",
journal = "The Journal of Physiology",
issn = "0022-3751",
publisher = "Wiley-Blackwell",

}

RIS

TY - JOUR

T1 - Facilitation of plateau potentials in turtle motoneurones by a pathway dependent on calcium and calmodulin

AU - Perrier, J F

AU - Mejia-Gervacio, S

AU - Hounsgaard, J

PY - 2000/10/1

Y1 - 2000/10/1

N2 - 1. The involvement of intracellular calcium and calmodulin in the modulation of plateau potentials in motoneurones was investigated using intracellular recordings from a spinal cord slice preparation. 2. Chelation of intracellular calcium with BAPTA-AM or inactivation of calmodulin with W-7 or trifluoperazine reduced the amplitude of depolarization-induced plateau potentials. Inactivation of calmodulin also inhibited facilitation of plateau potentials by activation of group I metabotropic glutamate receptors or muscarinic receptors. 3. In low-sodium medium and in the presence of tetraethylammonium and tetrodotoxin, calcium action potentials evoked by depolarization were followed by a short hyperpolarization ascribed to the calcium-activated non-selective cationic current (ICAN) and by a dihydropyridine-sensitive afterdepolarization. The amplitude of the afterdepolarization depended on the number of calcium spikes and was mediated by L-type calcium channels. 4. The dihydropyridine-sensitive afterdepolarization induced by calcium spikes was reduced by blockade of calmodulin. 5. It is proposed that plateau potentials in spinal motoneurones are facilitated by activation of a calcium-calmodulin-dependent pathway.

AB - 1. The involvement of intracellular calcium and calmodulin in the modulation of plateau potentials in motoneurones was investigated using intracellular recordings from a spinal cord slice preparation. 2. Chelation of intracellular calcium with BAPTA-AM or inactivation of calmodulin with W-7 or trifluoperazine reduced the amplitude of depolarization-induced plateau potentials. Inactivation of calmodulin also inhibited facilitation of plateau potentials by activation of group I metabotropic glutamate receptors or muscarinic receptors. 3. In low-sodium medium and in the presence of tetraethylammonium and tetrodotoxin, calcium action potentials evoked by depolarization were followed by a short hyperpolarization ascribed to the calcium-activated non-selective cationic current (ICAN) and by a dihydropyridine-sensitive afterdepolarization. The amplitude of the afterdepolarization depended on the number of calcium spikes and was mediated by L-type calcium channels. 4. The dihydropyridine-sensitive afterdepolarization induced by calcium spikes was reduced by blockade of calmodulin. 5. It is proposed that plateau potentials in spinal motoneurones are facilitated by activation of a calcium-calmodulin-dependent pathway.

KW - Animals

KW - Calcium

KW - Calcium Channels, L-Type

KW - Calmodulin

KW - Dopamine Antagonists

KW - Egtazic Acid

KW - Intracellular Fluid

KW - Membrane Potentials

KW - Motor Neurons

KW - Receptors, Metabotropic Glutamate

KW - Receptors, Muscarinic

KW - Sodium

KW - Spinal Cord

KW - Tetraethylammonium

KW - Tetrodotoxin

KW - Trifluoperazine

KW - Turtles

M3 - Journal article

C2 - 11018109

VL - 528 Pt 1

SP - 107

EP - 113

JO - The Journal of Physiology

JF - The Journal of Physiology

SN - 0022-3751

ER -

ID: 33731465