Expression of presynaptic markers in a neurodevelopmental animal model with relevance to schizophrenia

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Expression of presynaptic markers in a neurodevelopmental animal model with relevance to schizophrenia. / Karlsen, Anna S; Kaalund, Sanne Simone; Møller, Morten; Plath, Niels; Pakkenberg, Bente.

In: NeuroReport, Vol. 24, No. 16, 13.11.2013, p. 928-933.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Karlsen, AS, Kaalund, SS, Møller, M, Plath, N & Pakkenberg, B 2013, 'Expression of presynaptic markers in a neurodevelopmental animal model with relevance to schizophrenia', NeuroReport, vol. 24, no. 16, pp. 928-933. https://doi.org/10.1097/WNR.0000000000000030

APA

Karlsen, A. S., Kaalund, S. S., Møller, M., Plath, N., & Pakkenberg, B. (2013). Expression of presynaptic markers in a neurodevelopmental animal model with relevance to schizophrenia. NeuroReport, 24(16), 928-933. https://doi.org/10.1097/WNR.0000000000000030

Vancouver

Karlsen AS, Kaalund SS, Møller M, Plath N, Pakkenberg B. Expression of presynaptic markers in a neurodevelopmental animal model with relevance to schizophrenia. NeuroReport. 2013 Nov 13;24(16):928-933. https://doi.org/10.1097/WNR.0000000000000030

Author

Karlsen, Anna S ; Kaalund, Sanne Simone ; Møller, Morten ; Plath, Niels ; Pakkenberg, Bente. / Expression of presynaptic markers in a neurodevelopmental animal model with relevance to schizophrenia. In: NeuroReport. 2013 ; Vol. 24, No. 16. pp. 928-933.

Bibtex

@article{e012fa73ee334b24a76e434dfc8d82d9,
title = "Expression of presynaptic markers in a neurodevelopmental animal model with relevance to schizophrenia",
abstract = "Administration of N-methyl-D-aspartate receptor antagonist phencyclidine (PCP) to rat pups at postnatal day (PND) 7, 9, and 11 [neonatal PCP (neoPCP) model] induces cognitive deficits similar to those observed in schizophrenia. Expression of presynaptic SNARE protein, synaptosomal-associated protein of 25 kDa (Snap25), has been shown to be downregulated in postmortem brains from patients with schizophrenia. The present study was designed to investigate the long-term effects of neoPCP administration on expression of presynaptic markers altered in schizophrenia. Using radioactive in-situ hybridization, the expression of Snap25 was measured in the prefrontal cortex and the hippocampal formation (CA1, CA3, CA4, and dentate gyrus) at PND 29 and 80 in neoPCP and control rats. As a secondary presynaptic marker, the expressional level of synaptophysin was also measured in the same areas. Stereological estimation of the number of neurons and volume was used to exclude potential bias in cell numbers. A significant reduction in the expression of Snap25 in the hippocampal CA4 region was observed in adult neoPCP rats (PND 80, P",
author = "Karlsen, {Anna S} and Kaalund, {Sanne Simone} and Morten M{\o}ller and Niels Plath and Bente Pakkenberg",
year = "2013",
month = nov,
day = "13",
doi = "10.1097/WNR.0000000000000030",
language = "English",
volume = "24",
pages = "928--933",
journal = "NeuroReport",
issn = "0959-4965",
publisher = "Lippincott Williams & Wilkins",
number = "16",

}

RIS

TY - JOUR

T1 - Expression of presynaptic markers in a neurodevelopmental animal model with relevance to schizophrenia

AU - Karlsen, Anna S

AU - Kaalund, Sanne Simone

AU - Møller, Morten

AU - Plath, Niels

AU - Pakkenberg, Bente

PY - 2013/11/13

Y1 - 2013/11/13

N2 - Administration of N-methyl-D-aspartate receptor antagonist phencyclidine (PCP) to rat pups at postnatal day (PND) 7, 9, and 11 [neonatal PCP (neoPCP) model] induces cognitive deficits similar to those observed in schizophrenia. Expression of presynaptic SNARE protein, synaptosomal-associated protein of 25 kDa (Snap25), has been shown to be downregulated in postmortem brains from patients with schizophrenia. The present study was designed to investigate the long-term effects of neoPCP administration on expression of presynaptic markers altered in schizophrenia. Using radioactive in-situ hybridization, the expression of Snap25 was measured in the prefrontal cortex and the hippocampal formation (CA1, CA3, CA4, and dentate gyrus) at PND 29 and 80 in neoPCP and control rats. As a secondary presynaptic marker, the expressional level of synaptophysin was also measured in the same areas. Stereological estimation of the number of neurons and volume was used to exclude potential bias in cell numbers. A significant reduction in the expression of Snap25 in the hippocampal CA4 region was observed in adult neoPCP rats (PND 80, P

AB - Administration of N-methyl-D-aspartate receptor antagonist phencyclidine (PCP) to rat pups at postnatal day (PND) 7, 9, and 11 [neonatal PCP (neoPCP) model] induces cognitive deficits similar to those observed in schizophrenia. Expression of presynaptic SNARE protein, synaptosomal-associated protein of 25 kDa (Snap25), has been shown to be downregulated in postmortem brains from patients with schizophrenia. The present study was designed to investigate the long-term effects of neoPCP administration on expression of presynaptic markers altered in schizophrenia. Using radioactive in-situ hybridization, the expression of Snap25 was measured in the prefrontal cortex and the hippocampal formation (CA1, CA3, CA4, and dentate gyrus) at PND 29 and 80 in neoPCP and control rats. As a secondary presynaptic marker, the expressional level of synaptophysin was also measured in the same areas. Stereological estimation of the number of neurons and volume was used to exclude potential bias in cell numbers. A significant reduction in the expression of Snap25 in the hippocampal CA4 region was observed in adult neoPCP rats (PND 80, P

U2 - 10.1097/WNR.0000000000000030

DO - 10.1097/WNR.0000000000000030

M3 - Journal article

C2 - 24045778

VL - 24

SP - 928

EP - 933

JO - NeuroReport

JF - NeuroReport

SN - 0959-4965

IS - 16

ER -

ID: 106813106