Enriched environment reduces apolipoprotein E (ApoE) in reactive astrocytes and attenuates inflammation of the peri-infarct tissue after experimental stroke
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Enriched environment reduces apolipoprotein E (ApoE) in reactive astrocytes and attenuates inflammation of the peri-infarct tissue after experimental stroke. / Ruscher, Karsten; Johannesson, Emelie; Brugiere, Elena; Erickson, Agnes; Rickhag, Karl Mattias; Wieloch, Tadeusz.
In: Journal of Cerebral Blood Flow and Metabolism, Vol. 29, No. 11, 11.2009, p. 1796-805.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Enriched environment reduces apolipoprotein E (ApoE) in reactive astrocytes and attenuates inflammation of the peri-infarct tissue after experimental stroke
AU - Ruscher, Karsten
AU - Johannesson, Emelie
AU - Brugiere, Elena
AU - Erickson, Agnes
AU - Rickhag, Karl Mattias
AU - Wieloch, Tadeusz
PY - 2009/11
Y1 - 2009/11
N2 - Apolipoprotein E (ApoE), a cholesterol transporter and an immunomodulator, is brain protective after experimental stroke and implicated in brain repair. Here, we study the involvement of ApoE in the restoration of brain function after experimental stroke, by using animal housing conditions that differentially improve recovery after occlusion of the middle cerebral artery occlusion (MCAO). We found that after MCAO the ApoE levels increased in the injured hemisphere over a 30 days recovery period. The exception was a proximal narrow peri-infarct rim, in which ApoE was solely localized in S100beta(+)/glial fibrillary acidic protein (GFAP) negative reactive astrocytes at 4 to 7 days of recovery. Enriched housing after MCAO caused a marked decrease in ApoE levels compared with standard housing conditions, particularly in the ApoE/S100beta(+) reactive astrocytes. In addition, the levels of interleukin 1beta were lower in animals housed in an enriched environment. We propose that during the subacute phase after experimental stroke a zone for tissue reorganization with low cellular ApoE levels is formed. We conclude that the strong sensori-motor stimulation provided by enriched housing conditions mitigates the inflammatory response after stroke decreasing the level of ApoE that may contribute to the observed improvement of functional recovery.
AB - Apolipoprotein E (ApoE), a cholesterol transporter and an immunomodulator, is brain protective after experimental stroke and implicated in brain repair. Here, we study the involvement of ApoE in the restoration of brain function after experimental stroke, by using animal housing conditions that differentially improve recovery after occlusion of the middle cerebral artery occlusion (MCAO). We found that after MCAO the ApoE levels increased in the injured hemisphere over a 30 days recovery period. The exception was a proximal narrow peri-infarct rim, in which ApoE was solely localized in S100beta(+)/glial fibrillary acidic protein (GFAP) negative reactive astrocytes at 4 to 7 days of recovery. Enriched housing after MCAO caused a marked decrease in ApoE levels compared with standard housing conditions, particularly in the ApoE/S100beta(+) reactive astrocytes. In addition, the levels of interleukin 1beta were lower in animals housed in an enriched environment. We propose that during the subacute phase after experimental stroke a zone for tissue reorganization with low cellular ApoE levels is formed. We conclude that the strong sensori-motor stimulation provided by enriched housing conditions mitigates the inflammatory response after stroke decreasing the level of ApoE that may contribute to the observed improvement of functional recovery.
KW - Animals
KW - Apolipoproteins E
KW - Astrocytes
KW - Behavior, Animal
KW - Blotting, Western
KW - Brain
KW - Brain Infarction
KW - Cell Count
KW - Encephalitis
KW - Environment
KW - Enzyme-Linked Immunosorbent Assay
KW - Housing, Animal
KW - Immunohistochemistry
KW - Male
KW - Rats
KW - Rats, Wistar
KW - Stroke
U2 - 10.1038/jcbfm.2009.96
DO - 10.1038/jcbfm.2009.96
M3 - Journal article
C2 - 19623195
VL - 29
SP - 1796
EP - 1805
JO - Journal of Cerebral Blood Flow and Metabolism
JF - Journal of Cerebral Blood Flow and Metabolism
SN - 0271-678X
IS - 11
ER -
ID: 132931263