Endogenous IFN-β signaling exerts anti-inflammatory actions in experimentally induced focal cerebral ischemia
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Endogenous IFN-β signaling exerts anti-inflammatory actions in experimentally induced focal cerebral ischemia. / Inácio, Ana R; Liu, Yawei; Clausen, Bettina H; Svensson, Martina; Kucharz, Krzysztof; Yang, Yiyi; Stankovich, Totte; Khorooshi, Reza; Lambertsen, Kate L; Issazadeh-Navikas, Shohreh; Deierborg, Tomas.
In: Journal of Neuroinflammation, Vol. 12, 211, 2015, p. 1-18.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Endogenous IFN-β signaling exerts anti-inflammatory actions in experimentally induced focal cerebral ischemia
AU - Inácio, Ana R
AU - Liu, Yawei
AU - Clausen, Bettina H
AU - Svensson, Martina
AU - Kucharz, Krzysztof
AU - Yang, Yiyi
AU - Stankovich, Totte
AU - Khorooshi, Reza
AU - Lambertsen, Kate L
AU - Issazadeh-Navikas, Shohreh
AU - Deierborg, Tomas
PY - 2015
Y1 - 2015
N2 - BACKGROUND: Interferon (IFN)-β exerts anti-inflammatory effects, coupled to remarkable neurological improvements in multiple sclerosis, a neuroinflammatory condition of the central nervous system. Analogously, it has been hypothesized that IFN-β, by limiting inflammation, decreases neuronal death and promotes functional recovery after stroke. However, the core actions of endogenous IFN-β signaling in stroke are unclear.METHODS: To address this question, we used two clinically relevant models of focal cerebral ischemia, transient and permanent middle cerebral artery occlusion, and two genetically modified mouse lines, lacking either IFN-β or its receptor, the IFN-α/β receptor. Subsets of inflammatory and immune cells isolated from the brain, blood, and spleen were studied using flow cytometry. Sensorimotor deficits were assessed by a modified composite neuroscore, the rotating pole and grip strength tests, and cerebral infarct volumes were given by lack of neuronal nuclei immunoreactivity.RESULTS: Here, we report alterations in local and systemic inflammation in IFN-β knockout (IFN-βKO) mice over 8 days after induction of focal cerebral ischemia. Notably, IFN-βKO mice showed a higher number of infiltrating leukocytes in the brain 2 days after stroke. Concomitantly, in the blood of IFN-βKO mice, we found a higher percentage of total B cells but a similar percentage of mature and activated B cells, collectively indicating a higher proliferation rate. The additional differential regulation of circulating cytokines and splenic immune cell populations in wild-type and IFN-βKO mice further supports an important immunoregulatory function of IFN-β in stroke. Moreover, we observed a significant weight loss 2-3 days and a reduction in grip strength 2 days after stroke in the IFN-βKO group, while endogenous IFN-β signaling did not affect the infarct volume.CONCLUSIONS: We conclude that endogenous IFN-β signaling attenuates local inflammation, regulates peripheral immune cells, and, thereby, may contribute positively to stroke outcome.
AB - BACKGROUND: Interferon (IFN)-β exerts anti-inflammatory effects, coupled to remarkable neurological improvements in multiple sclerosis, a neuroinflammatory condition of the central nervous system. Analogously, it has been hypothesized that IFN-β, by limiting inflammation, decreases neuronal death and promotes functional recovery after stroke. However, the core actions of endogenous IFN-β signaling in stroke are unclear.METHODS: To address this question, we used two clinically relevant models of focal cerebral ischemia, transient and permanent middle cerebral artery occlusion, and two genetically modified mouse lines, lacking either IFN-β or its receptor, the IFN-α/β receptor. Subsets of inflammatory and immune cells isolated from the brain, blood, and spleen were studied using flow cytometry. Sensorimotor deficits were assessed by a modified composite neuroscore, the rotating pole and grip strength tests, and cerebral infarct volumes were given by lack of neuronal nuclei immunoreactivity.RESULTS: Here, we report alterations in local and systemic inflammation in IFN-β knockout (IFN-βKO) mice over 8 days after induction of focal cerebral ischemia. Notably, IFN-βKO mice showed a higher number of infiltrating leukocytes in the brain 2 days after stroke. Concomitantly, in the blood of IFN-βKO mice, we found a higher percentage of total B cells but a similar percentage of mature and activated B cells, collectively indicating a higher proliferation rate. The additional differential regulation of circulating cytokines and splenic immune cell populations in wild-type and IFN-βKO mice further supports an important immunoregulatory function of IFN-β in stroke. Moreover, we observed a significant weight loss 2-3 days and a reduction in grip strength 2 days after stroke in the IFN-βKO group, while endogenous IFN-β signaling did not affect the infarct volume.CONCLUSIONS: We conclude that endogenous IFN-β signaling attenuates local inflammation, regulates peripheral immune cells, and, thereby, may contribute positively to stroke outcome.
KW - Animals
KW - B-Lymphocytes
KW - Brain
KW - Brain Ischemia
KW - Cytokines
KW - Hand Strength
KW - Infarction, Middle Cerebral Artery
KW - Inflammation
KW - Interferon-beta
KW - Ischemic Attack, Transient
KW - Leukocytes
KW - Lymphocyte Count
KW - Male
KW - Mice
KW - Mice, Inbred C57BL
KW - Mice, Knockout
KW - Postural Balance
KW - Receptors, Interferon
KW - Spleen
KW - Stroke
U2 - 10.1186/s12974-015-0427-0
DO - 10.1186/s12974-015-0427-0
M3 - Journal article
C2 - 26581581
VL - 12
SP - 1
EP - 18
JO - Journal of Neuroinflammation
JF - Journal of Neuroinflammation
SN - 1742-2094
M1 - 211
ER -
ID: 161158547