Electron microscopy of the mouse central nervous system

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research

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Electron microscopy of the mouse central nervous system. / Möbius, Wiebke; Cooper, Benjamin; Kaufmann, Walter A; Imig, Cordelia; Ruhwedel, Torben; Snaidero, Nicolas; Saab, Aiman S; Varoqueaux, Frédérique.

Electron Microscopy of Model Systems. Vol. 96 2010. p. 475-512 (Methods in Cell Biology).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research

Harvard

Möbius, W, Cooper, B, Kaufmann, WA, Imig, C, Ruhwedel, T, Snaidero, N, Saab, AS & Varoqueaux, F 2010, Electron microscopy of the mouse central nervous system. in Electron Microscopy of Model Systems. vol. 96, Methods in Cell Biology, pp. 475-512. https://doi.org/10.1016/S0091-679X(10)96020-2

APA

Möbius, W., Cooper, B., Kaufmann, W. A., Imig, C., Ruhwedel, T., Snaidero, N., Saab, A. S., & Varoqueaux, F. (2010). Electron microscopy of the mouse central nervous system. In Electron Microscopy of Model Systems (Vol. 96, pp. 475-512). Methods in Cell Biology https://doi.org/10.1016/S0091-679X(10)96020-2

Vancouver

Möbius W, Cooper B, Kaufmann WA, Imig C, Ruhwedel T, Snaidero N et al. Electron microscopy of the mouse central nervous system. In Electron Microscopy of Model Systems. Vol. 96. 2010. p. 475-512. (Methods in Cell Biology). https://doi.org/10.1016/S0091-679X(10)96020-2

Author

Möbius, Wiebke ; Cooper, Benjamin ; Kaufmann, Walter A ; Imig, Cordelia ; Ruhwedel, Torben ; Snaidero, Nicolas ; Saab, Aiman S ; Varoqueaux, Frédérique. / Electron microscopy of the mouse central nervous system. Electron Microscopy of Model Systems. Vol. 96 2010. pp. 475-512 (Methods in Cell Biology).

Bibtex

@inbook{cb795a72ee724080b8ce43d90f4fc3cc,

title = "Electron microscopy of the mouse central nervous system",

abstract = "The high degree of similarity between mouse and human physiology and genomes makes mice the animal model of choice to study the functions and dysfunctions of the central nervous system (CNS). The considerable knowledge accumulated in the past decades and the steadily growing number of genetically modified mouse lines allow for the increasingly accurate understanding of biological circuits. Electron microscopy (EM) contributes to unravel the biology of neuronal networks and the myelinating glia by allowing a fine morphological scrutiny of the nervous tissue. We provide detailed descriptions of the conventional processing as well as cryopreparation methods such as high-pressure freezing (HPF), freeze-substitution (FS), and SDS-digested freeze-fracture replica labeling (SDS-FRL) on selected CNS regions such as the retina, optic nerve, and cerebellum. By taking example of the ribbon synapse in the retina and myelinated retinal ganglion cell axons of the optic nerve, we discuss the advantages and drawbacks of these methods in a comparative way.",

keywords = "Animals, Central Nervous System/ultrastructure, Freeze Fracturing/methods, Freeze Substitution/methods, Humans, Immunohistochemistry, Mice/anatomy & histology, Microscopy, Electron/instrumentation, Retina/ultrastructure, Staining and Labeling/methods, Tissue Fixation/methods",

author = "Wiebke M{\"o}bius and Benjamin Cooper and Kaufmann, {Walter A} and Cordelia Imig and Torben Ruhwedel and Nicolas Snaidero and Saab, {Aiman S} and Fr{\'e}d{\'e}rique Varoqueaux",

year = "2010",

doi = "10.1016/S0091-679X(10)96020-2",

language = "English",

volume = "96",

series = "Methods in Cell Biology",

publisher = "Academic Press",

pages = "475--512",

booktitle = "Electron Microscopy of Model Systems",

}

RIS

TY - CHAP

T1 - Electron microscopy of the mouse central nervous system

AU - Möbius, Wiebke

AU - Cooper, Benjamin

AU - Kaufmann, Walter A

AU - Imig, Cordelia

AU - Ruhwedel, Torben

AU - Snaidero, Nicolas

AU - Saab, Aiman S

AU - Varoqueaux, Frédérique

PY - 2010

Y1 - 2010

N2 - The high degree of similarity between mouse and human physiology and genomes makes mice the animal model of choice to study the functions and dysfunctions of the central nervous system (CNS). The considerable knowledge accumulated in the past decades and the steadily growing number of genetically modified mouse lines allow for the increasingly accurate understanding of biological circuits. Electron microscopy (EM) contributes to unravel the biology of neuronal networks and the myelinating glia by allowing a fine morphological scrutiny of the nervous tissue. We provide detailed descriptions of the conventional processing as well as cryopreparation methods such as high-pressure freezing (HPF), freeze-substitution (FS), and SDS-digested freeze-fracture replica labeling (SDS-FRL) on selected CNS regions such as the retina, optic nerve, and cerebellum. By taking example of the ribbon synapse in the retina and myelinated retinal ganglion cell axons of the optic nerve, we discuss the advantages and drawbacks of these methods in a comparative way.

AB - The high degree of similarity between mouse and human physiology and genomes makes mice the animal model of choice to study the functions and dysfunctions of the central nervous system (CNS). The considerable knowledge accumulated in the past decades and the steadily growing number of genetically modified mouse lines allow for the increasingly accurate understanding of biological circuits. Electron microscopy (EM) contributes to unravel the biology of neuronal networks and the myelinating glia by allowing a fine morphological scrutiny of the nervous tissue. We provide detailed descriptions of the conventional processing as well as cryopreparation methods such as high-pressure freezing (HPF), freeze-substitution (FS), and SDS-digested freeze-fracture replica labeling (SDS-FRL) on selected CNS regions such as the retina, optic nerve, and cerebellum. By taking example of the ribbon synapse in the retina and myelinated retinal ganglion cell axons of the optic nerve, we discuss the advantages and drawbacks of these methods in a comparative way.

KW - Animals

KW - Central Nervous System/ultrastructure

KW - Freeze Fracturing/methods

KW - Freeze Substitution/methods

KW - Humans

KW - Immunohistochemistry

KW - Mice/anatomy & histology

KW - Microscopy, Electron/instrumentation

KW - Retina/ultrastructure

KW - Staining and Labeling/methods

KW - Tissue Fixation/methods

U2 - 10.1016/S0091-679X(10)96020-2

DO - 10.1016/S0091-679X(10)96020-2

M3 - Book chapter

C2 - 20869535

VL - 96

T3 - Methods in Cell Biology

SP - 475

EP - 512

BT - Electron Microscopy of Model Systems

ER -

ID: 237698447

Department of Neuroscience