Dopaminergic neurons establish a distinctive axonal arbor with a majority of non-synaptic terminals

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Dopaminergic neurons establish a distinctive axonal arbor with a majority of non-synaptic terminals. / Ducrot, Charles; Bourque, Marie-Josee; Delmas, Constantin V. L.; Racine, Anne-Sophie; Guadarrama Bello, Dainelys; Delignat-Lavaud, Benoit; Domenic Lycas, Matthew; Fallon, Aurelie; Michaud-Tardif, Charlotte; Burke Nanni, Samuel; Herborg, Freja; Gether, Ulrik; Nanci, Antonio; Takahashi, Hideto; Parent, Martin; Trudeau, Louis-Eric.

In: FASEB Journal, Vol. 35, No. 8, 21791, 2021.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Ducrot, C, Bourque, M-J, Delmas, CVL, Racine, A-S, Guadarrama Bello, D, Delignat-Lavaud, B, Domenic Lycas, M, Fallon, A, Michaud-Tardif, C, Burke Nanni, S, Herborg, F, Gether, U, Nanci, A, Takahashi, H, Parent, M & Trudeau, L-E 2021, 'Dopaminergic neurons establish a distinctive axonal arbor with a majority of non-synaptic terminals', FASEB Journal, vol. 35, no. 8, 21791. https://doi.org/10.1096/fj.202100201RR

APA

Ducrot, C., Bourque, M-J., Delmas, C. V. L., Racine, A-S., Guadarrama Bello, D., Delignat-Lavaud, B., Domenic Lycas, M., Fallon, A., Michaud-Tardif, C., Burke Nanni, S., Herborg, F., Gether, U., Nanci, A., Takahashi, H., Parent, M., & Trudeau, L-E. (2021). Dopaminergic neurons establish a distinctive axonal arbor with a majority of non-synaptic terminals. FASEB Journal, 35(8), [21791]. https://doi.org/10.1096/fj.202100201RR

Vancouver

Ducrot C, Bourque M-J, Delmas CVL, Racine A-S, Guadarrama Bello D, Delignat-Lavaud B et al. Dopaminergic neurons establish a distinctive axonal arbor with a majority of non-synaptic terminals. FASEB Journal. 2021;35(8). 21791. https://doi.org/10.1096/fj.202100201RR

Author

Ducrot, Charles ; Bourque, Marie-Josee ; Delmas, Constantin V. L. ; Racine, Anne-Sophie ; Guadarrama Bello, Dainelys ; Delignat-Lavaud, Benoit ; Domenic Lycas, Matthew ; Fallon, Aurelie ; Michaud-Tardif, Charlotte ; Burke Nanni, Samuel ; Herborg, Freja ; Gether, Ulrik ; Nanci, Antonio ; Takahashi, Hideto ; Parent, Martin ; Trudeau, Louis-Eric. / Dopaminergic neurons establish a distinctive axonal arbor with a majority of non-synaptic terminals. In: FASEB Journal. 2021 ; Vol. 35, No. 8.

Bibtex

@article{1efde3fa9c3344e09bc14db78b7679d1,
title = "Dopaminergic neurons establish a distinctive axonal arbor with a majority of non-synaptic terminals",
abstract = "Chemical neurotransmission typically occurs through synapses. Previous ultrastructural examinations of monoamine neuron axon terminals often failed to identify a pre- and postsynaptic coupling, leading to the concept of {"}volume{"} transmission. Whether this results from intrinsic properties of these neurons remains undefined. We find that dopaminergic neurons in vitro establish a distinctive axonal arbor compared to glutamatergic or GABAergic neurons in both size and propensity of terminals to avoid direct contact with target neurons. While most dopaminergic varicosities are active and contain exocytosis proteins like synaptotagmin 1, only similar to 20% of these are synaptic. The active zone protein bassoon was found to be enriched in dopaminergic terminals that are in proximity to a target cell. Finally, we found that the proteins neurexin-1 alpha(SS4-) and neuroligin-1(A+B) play a critical role in the formation of synapses by dopamine (DA) neurons. Our findings suggest that DA neurons are endowed with a distinctive developmental connectivity program.",
keywords = "active zone, axon terminals, dopamine, exocytosis, synapse, volume transmission, PRESYNAPTIC ACTIVE ZONES, GLUTAMATE CORELEASE, ULTRASTRUCTURAL-LOCALIZATION, TYROSINE-HYDROXYLASE, NEUROLIGIN 1, RELEASE, SYNAPSES, INNERVATION, TRANSMISSION, EXPRESSION",
author = "Charles Ducrot and Marie-Josee Bourque and Delmas, {Constantin V. L.} and Anne-Sophie Racine and {Guadarrama Bello}, Dainelys and Benoit Delignat-Lavaud and {Domenic Lycas}, Matthew and Aurelie Fallon and Charlotte Michaud-Tardif and {Burke Nanni}, Samuel and Freja Herborg and Ulrik Gether and Antonio Nanci and Hideto Takahashi and Martin Parent and Louis-Eric Trudeau",
year = "2021",
doi = "10.1096/fj.202100201RR",
language = "English",
volume = "35",
journal = "F A S E B Journal",
issn = "0892-6638",
publisher = "Federation of American Societies for Experimental Biology",
number = "8",

}

RIS

TY - JOUR

T1 - Dopaminergic neurons establish a distinctive axonal arbor with a majority of non-synaptic terminals

AU - Ducrot, Charles

AU - Bourque, Marie-Josee

AU - Delmas, Constantin V. L.

AU - Racine, Anne-Sophie

AU - Guadarrama Bello, Dainelys

AU - Delignat-Lavaud, Benoit

AU - Domenic Lycas, Matthew

AU - Fallon, Aurelie

AU - Michaud-Tardif, Charlotte

AU - Burke Nanni, Samuel

AU - Herborg, Freja

AU - Gether, Ulrik

AU - Nanci, Antonio

AU - Takahashi, Hideto

AU - Parent, Martin

AU - Trudeau, Louis-Eric

PY - 2021

Y1 - 2021

N2 - Chemical neurotransmission typically occurs through synapses. Previous ultrastructural examinations of monoamine neuron axon terminals often failed to identify a pre- and postsynaptic coupling, leading to the concept of "volume" transmission. Whether this results from intrinsic properties of these neurons remains undefined. We find that dopaminergic neurons in vitro establish a distinctive axonal arbor compared to glutamatergic or GABAergic neurons in both size and propensity of terminals to avoid direct contact with target neurons. While most dopaminergic varicosities are active and contain exocytosis proteins like synaptotagmin 1, only similar to 20% of these are synaptic. The active zone protein bassoon was found to be enriched in dopaminergic terminals that are in proximity to a target cell. Finally, we found that the proteins neurexin-1 alpha(SS4-) and neuroligin-1(A+B) play a critical role in the formation of synapses by dopamine (DA) neurons. Our findings suggest that DA neurons are endowed with a distinctive developmental connectivity program.

AB - Chemical neurotransmission typically occurs through synapses. Previous ultrastructural examinations of monoamine neuron axon terminals often failed to identify a pre- and postsynaptic coupling, leading to the concept of "volume" transmission. Whether this results from intrinsic properties of these neurons remains undefined. We find that dopaminergic neurons in vitro establish a distinctive axonal arbor compared to glutamatergic or GABAergic neurons in both size and propensity of terminals to avoid direct contact with target neurons. While most dopaminergic varicosities are active and contain exocytosis proteins like synaptotagmin 1, only similar to 20% of these are synaptic. The active zone protein bassoon was found to be enriched in dopaminergic terminals that are in proximity to a target cell. Finally, we found that the proteins neurexin-1 alpha(SS4-) and neuroligin-1(A+B) play a critical role in the formation of synapses by dopamine (DA) neurons. Our findings suggest that DA neurons are endowed with a distinctive developmental connectivity program.

KW - active zone

KW - axon terminals

KW - dopamine

KW - exocytosis

KW - synapse

KW - volume transmission

KW - PRESYNAPTIC ACTIVE ZONES

KW - GLUTAMATE CORELEASE

KW - ULTRASTRUCTURAL-LOCALIZATION

KW - TYROSINE-HYDROXYLASE

KW - NEUROLIGIN 1

KW - RELEASE

KW - SYNAPSES

KW - INNERVATION

KW - TRANSMISSION

KW - EXPRESSION

U2 - 10.1096/fj.202100201RR

DO - 10.1096/fj.202100201RR

M3 - Journal article

C2 - 34320240

VL - 35

JO - F A S E B Journal

JF - F A S E B Journal

SN - 0892-6638

IS - 8

M1 - 21791

ER -

ID: 276228561