TY - JOUR

T1 - Diurnal expression of proteins in the retina of the blind cone-rod homeobox (Crx−/−) mouse and the 129/Sv mouse

T2 - a proteomic study

AU - Møller, Morten

AU - Rath, Martin Fredensborg

AU - Ludvigsen, Maja

AU - Honoré, Bent

AU - Vorum, Henrik

PY - 2017/11

Y1 - 2017/11

N2 - Purpose: The vertebrate retina contains a circadian clock participating in adaptations to day and night vision. This peripheral clock is independent of the master clock in the suprachiasmatic nucleus (SCN). The retinal clock is located in several cell types, including the photoreceptors. To investigate the role of the circadian clock of the photoreceptor cells in regulation of retinal protein rhythms, we analysed diurnal protein expression in the photoreceptor-deficient cone-rod homeobox knockout mouse (Crx−/−) and the 129/Sv mouse. Methods: 2D gels were made from retinal homogenates of 129/Sv and Crx−/− mice killed at midday and midnight. Stained gels were analysed by use of PDQuest 2D gel analysis software. After trypsin digestion of differential expressed spots, the proteins were identified by LC-MS/MS using a nano-liquid chromatograph connected to a Q-TOF Premier mass spectrometer. These data were used to search the SWISS-PROT database. Results: Both the retinae of the control and the Crx−/− mice exhibited diurnal proteins rhythms. As expected, proteins involved in phototransduction were not detected in the Crx−/− mouse; in this phenotype, however, proteins from spots showing diurnal rhythms were specifically identified as enzymes involved in glucose metabolism, Krebs cycle, and mitochondrial enzymes. Data are available via ProteomeXchange with identifier PXD005556. Conclusion: We show diurnal protein rhythms in the retina of a mouse lacking the rods and cones. The diurnal protein rhythms in this genotype, lacking the circadian clock of the photoreceptors, might be caused by a circadian clock in other retinal cell types or a direct light input to the retina.

AB - Purpose: The vertebrate retina contains a circadian clock participating in adaptations to day and night vision. This peripheral clock is independent of the master clock in the suprachiasmatic nucleus (SCN). The retinal clock is located in several cell types, including the photoreceptors. To investigate the role of the circadian clock of the photoreceptor cells in regulation of retinal protein rhythms, we analysed diurnal protein expression in the photoreceptor-deficient cone-rod homeobox knockout mouse (Crx−/−) and the 129/Sv mouse. Methods: 2D gels were made from retinal homogenates of 129/Sv and Crx−/− mice killed at midday and midnight. Stained gels were analysed by use of PDQuest 2D gel analysis software. After trypsin digestion of differential expressed spots, the proteins were identified by LC-MS/MS using a nano-liquid chromatograph connected to a Q-TOF Premier mass spectrometer. These data were used to search the SWISS-PROT database. Results: Both the retinae of the control and the Crx−/− mice exhibited diurnal proteins rhythms. As expected, proteins involved in phototransduction were not detected in the Crx−/− mouse; in this phenotype, however, proteins from spots showing diurnal rhythms were specifically identified as enzymes involved in glucose metabolism, Krebs cycle, and mitochondrial enzymes. Data are available via ProteomeXchange with identifier PXD005556. Conclusion: We show diurnal protein rhythms in the retina of a mouse lacking the rods and cones. The diurnal protein rhythms in this genotype, lacking the circadian clock of the photoreceptors, might be caused by a circadian clock in other retinal cell types or a direct light input to the retina.

KW - blind

KW - circadian

KW - clock

KW - mouse

KW - retina

U2 - 10.1111/aos.13429

DO - 10.1111/aos.13429

M3 - Journal article

C2 - 28371363

AN - SCOPUS:85017404516

VL - 95

SP - 717

EP - 726

JO - Acta Ophthalmologica

JF - Acta Ophthalmologica

SN - 1755-375X

IS - 7

ER -