Deoxycholate-Based Glycosides (DCGs) for Membrane Protein Stabilisation
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Deoxycholate-Based Glycosides (DCGs) for Membrane Protein Stabilisation. / Bae, Hyoung Eun; Gotfryd, Kamil; Thomas, Jennifer; Hussain, Hazrat; Ehsan, Muhammad; Go, Juyeon; Loland, Claus J; Byrne, Bernadette; Chae, Pil Seok.
In: ChemBioChem, Vol. 16, No. 10, 2015, p. 1454–1459.Research output: Contribution to journal › Letter › Research › peer-review
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TY - JOUR
T1 - Deoxycholate-Based Glycosides (DCGs) for Membrane Protein Stabilisation
AU - Bae, Hyoung Eun
AU - Gotfryd, Kamil
AU - Thomas, Jennifer
AU - Hussain, Hazrat
AU - Ehsan, Muhammad
AU - Go, Juyeon
AU - Loland, Claus J
AU - Byrne, Bernadette
AU - Chae, Pil Seok
N1 - © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
PY - 2015
Y1 - 2015
N2 - Detergents are an absolute requirement for studying the structure of membrane proteins. However, many conventional detergents fail to stabilise denaturation-sensitive membrane proteins, such as eukaryotic proteins and membrane protein complexes. New amphipathic agents with enhanced efficacy in stabilising membrane proteins will be helpful in overcoming the barriers to studying membrane protein structures. We have prepared a number of deoxycholate-based amphiphiles with carbohydrate head groups, designated deoxycholate-based glycosides (DCGs). These DCGs are the hydrophilic variants of previously reported deoxycholate-based N-oxides (DCAOs). Membrane proteins in these agents, particularly the branched diglucoside-bearing amphiphiles DCG-1 and DCG-2, displayed favourable behaviour compared to previously reported parent compounds (DCAOs) and conventional detergents (LDAO and DDM). Given their excellent properties, these agents should have significant potential for membrane protein studies.
AB - Detergents are an absolute requirement for studying the structure of membrane proteins. However, many conventional detergents fail to stabilise denaturation-sensitive membrane proteins, such as eukaryotic proteins and membrane protein complexes. New amphipathic agents with enhanced efficacy in stabilising membrane proteins will be helpful in overcoming the barriers to studying membrane protein structures. We have prepared a number of deoxycholate-based amphiphiles with carbohydrate head groups, designated deoxycholate-based glycosides (DCGs). These DCGs are the hydrophilic variants of previously reported deoxycholate-based N-oxides (DCAOs). Membrane proteins in these agents, particularly the branched diglucoside-bearing amphiphiles DCG-1 and DCG-2, displayed favourable behaviour compared to previously reported parent compounds (DCAOs) and conventional detergents (LDAO and DDM). Given their excellent properties, these agents should have significant potential for membrane protein studies.
U2 - 10.1002/cbic.201500151
DO - 10.1002/cbic.201500151
M3 - Letter
C2 - 25953685
VL - 16
SP - 1454
EP - 1459
JO - ChemBioChem
JF - ChemBioChem
SN - 1439-4227
IS - 10
ER -
ID: 137623678