Delivery of differentiation factors by mesoporous silica particles assists advanced differentiation of transplanted murine embryonic stem cells
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Delivery of differentiation factors by mesoporous silica particles assists advanced differentiation of transplanted murine embryonic stem cells. / Garcia-Bennett, Alfonso E; Kozhevnikova, Mariya; König, Niclas; Zhou, Chunfang; Leao, Richardson; Knöpfel, Thomas; Pankratova, Stanislava; Trolle, Carl; Berezin, Vladimir; Bock, Elisabeth; Aldskogius, Håkan; Kozlova, Elena N.
In: Stem Cells Translational Medicine, Vol. 2, No. 11, 11.2013, p. 906-15.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Delivery of differentiation factors by mesoporous silica particles assists advanced differentiation of transplanted murine embryonic stem cells
AU - Garcia-Bennett, Alfonso E
AU - Kozhevnikova, Mariya
AU - König, Niclas
AU - Zhou, Chunfang
AU - Leao, Richardson
AU - Knöpfel, Thomas
AU - Pankratova, Stanislava
AU - Trolle, Carl
AU - Berezin, Vladimir
AU - Bock, Elisabeth
AU - Aldskogius, Håkan
AU - Kozlova, Elena N
PY - 2013/11
Y1 - 2013/11
N2 - Stem cell transplantation holds great hope for the replacement of damaged cells in the nervous system. However, poor long-term survival after transplantation and insufficiently robust differentiation of stem cells into specialized cell types in vivo remain major obstacles for clinical application. Here, we report the development of a novel technological approach for the local delivery of exogenous trophic factor mimetics to transplanted cells using specifically designed silica nanoporous particles. We demonstrated that delivering Cintrofin and Gliafin, established peptide mimetics of the ciliary neurotrophic factor and glial cell line-derived neurotrophic factor, respectively, with these particles enabled not only robust functional differentiation of motor neurons from transplanted embryonic stem cells but also their long-term survival in vivo. We propose that the delivery of growth factors by mesoporous nanoparticles is a potentially versatile and widely applicable strategy for efficient differentiation and functional integration of stem cell derivatives upon transplantation.
AB - Stem cell transplantation holds great hope for the replacement of damaged cells in the nervous system. However, poor long-term survival after transplantation and insufficiently robust differentiation of stem cells into specialized cell types in vivo remain major obstacles for clinical application. Here, we report the development of a novel technological approach for the local delivery of exogenous trophic factor mimetics to transplanted cells using specifically designed silica nanoporous particles. We demonstrated that delivering Cintrofin and Gliafin, established peptide mimetics of the ciliary neurotrophic factor and glial cell line-derived neurotrophic factor, respectively, with these particles enabled not only robust functional differentiation of motor neurons from transplanted embryonic stem cells but also their long-term survival in vivo. We propose that the delivery of growth factors by mesoporous nanoparticles is a potentially versatile and widely applicable strategy for efficient differentiation and functional integration of stem cell derivatives upon transplantation.
KW - Amino Acid Sequence
KW - Animals
KW - Cell Differentiation
KW - Cell Line
KW - Cell Survival
KW - Ciliary Neurotrophic Factor
KW - Embryonic Stem Cells
KW - Glial Cell Line-Derived Neurotrophic Factor
KW - Mice
KW - Molecular Sequence Data
KW - Motor Neurons
KW - Nanoparticles
KW - Peptides
KW - Silicon Dioxide
KW - Stem Cell Transplantation
U2 - 10.5966/sctm.2013-0072
DO - 10.5966/sctm.2013-0072
M3 - Journal article
C2 - 24089415
VL - 2
SP - 906
EP - 915
JO - Stem cells translational medicine
JF - Stem cells translational medicine
SN - 2157-6564
IS - 11
ER -
ID: 118388682