Decerebrate mouse model for studies of the spinal cord circuits
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Decerebrate mouse model for studies of the spinal cord circuits. / Meehan, Claire Francesca; Mayr, Kyle A; Manuel, Marin; Nakanishi, Stan T; Whelan, Patrick J.
In: Nature Protocols, Vol. 12, No. 4, 2017, p. 732-747.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Decerebrate mouse model for studies of the spinal cord circuits
AU - Meehan, Claire Francesca
AU - Mayr, Kyle A
AU - Manuel, Marin
AU - Nakanishi, Stan T
AU - Whelan, Patrick J
PY - 2017
Y1 - 2017
N2 - The adult decerebrate mouse model (a mouse with the cerebrum removed) enables the study of sensory-motor integration and motor output from the spinal cord for several hours without compromising these functions with anesthesia. For example, the decerebrate mouse is ideal for examining locomotor behavior using intracellular recording approaches, which would not be possible using current anesthetized preparations. This protocol describes the steps required to achieve a low-blood-loss decerebration in the mouse and approaches for recording signals from spinal cord neurons with a focus on motoneurons. The protocol also describes an example application for the protocol: the evocation of spontaneous and actively driven stepping, including optimization of these behaviors in decerebrate mice. The time taken to prepare the animal and perform a decerebration takes ∼2 h, and the mice are viable for up to 3-8 h, which is ample time to perform most short-term procedures. These protocols can be modified for those interested in cardiovascular or respiratory function in addition to motor function and can be performed by trainees with some previous experience in animal surgery.
AB - The adult decerebrate mouse model (a mouse with the cerebrum removed) enables the study of sensory-motor integration and motor output from the spinal cord for several hours without compromising these functions with anesthesia. For example, the decerebrate mouse is ideal for examining locomotor behavior using intracellular recording approaches, which would not be possible using current anesthetized preparations. This protocol describes the steps required to achieve a low-blood-loss decerebration in the mouse and approaches for recording signals from spinal cord neurons with a focus on motoneurons. The protocol also describes an example application for the protocol: the evocation of spontaneous and actively driven stepping, including optimization of these behaviors in decerebrate mice. The time taken to prepare the animal and perform a decerebration takes ∼2 h, and the mice are viable for up to 3-8 h, which is ample time to perform most short-term procedures. These protocols can be modified for those interested in cardiovascular or respiratory function in addition to motor function and can be performed by trainees with some previous experience in animal surgery.
U2 - 10.1038/nprot.2017.001
DO - 10.1038/nprot.2017.001
M3 - Journal article
C2 - 28277546
VL - 12
SP - 732
EP - 747
JO - Nature Protocols
JF - Nature Protocols
SN - 1754-2189
IS - 4
ER -
ID: 176952210