CtBP1-Mediated Membrane Fission Contributes to Effective Recycling of Synaptic Vesicles

Research output: Contribution to journal › Journal article › Research › peer-review

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CtBP1-Mediated Membrane Fission Contributes to Effective Recycling of Synaptic Vesicles. / Ivanova, Daniela; Imig, Cordelia; Camacho, Marcial; Reinhold, Annika; Guhathakurta, Debarpan; Montenegro-Venegas, Carolina; Cousin, Michael A; Gundelfinger, Eckart D; Rosenmund, Christian; Cooper, Benjamin; Fejtova, Anna.

In: Cell Reports, Vol. 30, No. 7, 18.02.2020, p. 2444-2459.e7.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Ivanova, D, Imig, C, Camacho, M, Reinhold, A, Guhathakurta, D, Montenegro-Venegas, C, Cousin, MA, Gundelfinger, ED, Rosenmund, C, Cooper, B & Fejtova, A 2020, 'CtBP1-Mediated Membrane Fission Contributes to Effective Recycling of Synaptic Vesicles', Cell Reports, vol. 30, no. 7, pp. 2444-2459.e7. https://doi.org/10.1016/j.celrep.2020.01.079

APA

Ivanova, D., Imig, C., Camacho, M., Reinhold, A., Guhathakurta, D., Montenegro-Venegas, C., Cousin, M. A., Gundelfinger, E. D., Rosenmund, C., Cooper, B., & Fejtova, A. (2020). CtBP1-Mediated Membrane Fission Contributes to Effective Recycling of Synaptic Vesicles. Cell Reports, 30(7), 2444-2459.e7. https://doi.org/10.1016/j.celrep.2020.01.079

Vancouver

Ivanova D, Imig C, Camacho M, Reinhold A, Guhathakurta D, Montenegro-Venegas C et al. CtBP1-Mediated Membrane Fission Contributes to Effective Recycling of Synaptic Vesicles. Cell Reports. 2020 Feb 18;30(7):2444-2459.e7. https://doi.org/10.1016/j.celrep.2020.01.079

Author

Ivanova, Daniela ; Imig, Cordelia ; Camacho, Marcial ; Reinhold, Annika ; Guhathakurta, Debarpan ; Montenegro-Venegas, Carolina ; Cousin, Michael A ; Gundelfinger, Eckart D ; Rosenmund, Christian ; Cooper, Benjamin ; Fejtova, Anna. / CtBP1-Mediated Membrane Fission Contributes to Effective Recycling of Synaptic Vesicles. In: Cell Reports. 2020 ; Vol. 30, No. 7. pp. 2444-2459.e7.

Bibtex

@article{5cbb5d396233452cb73bfa9075aa33f3,

title = "CtBP1-Mediated Membrane Fission Contributes to Effective Recycling of Synaptic Vesicles",

abstract = "Compensatory endocytosis of released synaptic vesicles (SVs) relies on coordinated signaling at the lipid-protein interface. Here, we address the synaptic function of C-terminal binding protein 1 (CtBP1), a ubiquitous regulator of gene expression and membrane trafficking in cultured hippocampal neurons. In the absence of CtBP1, synapses form in greater density and show changes in SV distribution and size. The increased basal neurotransmission and enhanced synaptic depression could be attributed to a higher vesicular release probability and a smaller fraction of release-competent SVs, respectively. Rescue experiments with specifically targeted constructs indicate that, while synaptogenesis and release probability are controlled by nuclear CtBP1, the efficient recycling of SVs relies on its synaptic expression. The ability of presynaptic CtBP1 to facilitate compensatory endocytosis depends on its membrane-fission activity and the activation of the lipid-metabolizing enzyme PLD1. Thus, CtBP1 regulates SV recycling by promoting a permissive lipid environment for compensatory endocytosis.",

author = "Daniela Ivanova and Cordelia Imig and Marcial Camacho and Annika Reinhold and Debarpan Guhathakurta and Carolina Montenegro-Venegas and Cousin, {Michael A} and Gundelfinger, {Eckart D} and Christian Rosenmund and Benjamin Cooper and Anna Fejtova",

year = "2020",

month = feb,

day = "18",

doi = "10.1016/j.celrep.2020.01.079",

language = "English",

volume = "30",

pages = "2444--2459.e7",

journal = "Cell Reports",

issn = "2211-1247",

publisher = "Cell Press",

number = "7",

}

RIS

TY - JOUR

T1 - CtBP1-Mediated Membrane Fission Contributes to Effective Recycling of Synaptic Vesicles

AU - Ivanova, Daniela

AU - Imig, Cordelia

AU - Camacho, Marcial

AU - Reinhold, Annika

AU - Guhathakurta, Debarpan

AU - Montenegro-Venegas, Carolina

AU - Cousin, Michael A

AU - Gundelfinger, Eckart D

AU - Rosenmund, Christian

AU - Cooper, Benjamin

AU - Fejtova, Anna

PY - 2020/2/18

Y1 - 2020/2/18

N2 - Compensatory endocytosis of released synaptic vesicles (SVs) relies on coordinated signaling at the lipid-protein interface. Here, we address the synaptic function of C-terminal binding protein 1 (CtBP1), a ubiquitous regulator of gene expression and membrane trafficking in cultured hippocampal neurons. In the absence of CtBP1, synapses form in greater density and show changes in SV distribution and size. The increased basal neurotransmission and enhanced synaptic depression could be attributed to a higher vesicular release probability and a smaller fraction of release-competent SVs, respectively. Rescue experiments with specifically targeted constructs indicate that, while synaptogenesis and release probability are controlled by nuclear CtBP1, the efficient recycling of SVs relies on its synaptic expression. The ability of presynaptic CtBP1 to facilitate compensatory endocytosis depends on its membrane-fission activity and the activation of the lipid-metabolizing enzyme PLD1. Thus, CtBP1 regulates SV recycling by promoting a permissive lipid environment for compensatory endocytosis.

AB - Compensatory endocytosis of released synaptic vesicles (SVs) relies on coordinated signaling at the lipid-protein interface. Here, we address the synaptic function of C-terminal binding protein 1 (CtBP1), a ubiquitous regulator of gene expression and membrane trafficking in cultured hippocampal neurons. In the absence of CtBP1, synapses form in greater density and show changes in SV distribution and size. The increased basal neurotransmission and enhanced synaptic depression could be attributed to a higher vesicular release probability and a smaller fraction of release-competent SVs, respectively. Rescue experiments with specifically targeted constructs indicate that, while synaptogenesis and release probability are controlled by nuclear CtBP1, the efficient recycling of SVs relies on its synaptic expression. The ability of presynaptic CtBP1 to facilitate compensatory endocytosis depends on its membrane-fission activity and the activation of the lipid-metabolizing enzyme PLD1. Thus, CtBP1 regulates SV recycling by promoting a permissive lipid environment for compensatory endocytosis.

U2 - 10.1016/j.celrep.2020.01.079

DO - 10.1016/j.celrep.2020.01.079

M3 - Journal article

C2 - 32075774

VL - 30

SP - 2444-2459.e7

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 7

ER -

ID: 237696785

Department of Neuroscience