Comprehensive regional and temporal gene expression profiling of the rat brain during the first 24 h after experimental stroke identifies dynamic ischemia-induced gene expression patterns, and reveals a biphasic activation of genes in surviving tissue

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Comprehensive regional and temporal gene expression profiling of the rat brain during the first 24 h after experimental stroke identifies dynamic ischemia-induced gene expression patterns, and reveals a biphasic activation of genes in surviving tissue. / Rickhag, Karl Mattias; Wieloch, Tadeusz; Gidö, Gunilla; Elmér, Eskil; Krogh, Morten; Murray, Joseph; Lohr, Scott; Bitter, Hans; Chin, Daniel J; von Schack, David; Shamloo, Mehrdad; Nikolich, Karoly.

In: Journal of Neurochemistry, Vol. 96, No. 1, 01.2006, p. 14-29.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Rickhag, KM, Wieloch, T, Gidö, G, Elmér, E, Krogh, M, Murray, J, Lohr, S, Bitter, H, Chin, DJ, von Schack, D, Shamloo, M & Nikolich, K 2006, 'Comprehensive regional and temporal gene expression profiling of the rat brain during the first 24 h after experimental stroke identifies dynamic ischemia-induced gene expression patterns, and reveals a biphasic activation of genes in surviving tissue', Journal of Neurochemistry, vol. 96, no. 1, pp. 14-29. https://doi.org/10.1111/j.1471-4159.2005.03508.x

APA

Rickhag, K. M., Wieloch, T., Gidö, G., Elmér, E., Krogh, M., Murray, J., Lohr, S., Bitter, H., Chin, D. J., von Schack, D., Shamloo, M., & Nikolich, K. (2006). Comprehensive regional and temporal gene expression profiling of the rat brain during the first 24 h after experimental stroke identifies dynamic ischemia-induced gene expression patterns, and reveals a biphasic activation of genes in surviving tissue. Journal of Neurochemistry, 96(1), 14-29. https://doi.org/10.1111/j.1471-4159.2005.03508.x

Vancouver

Rickhag KM, Wieloch T, Gidö G, Elmér E, Krogh M, Murray J et al. Comprehensive regional and temporal gene expression profiling of the rat brain during the first 24 h after experimental stroke identifies dynamic ischemia-induced gene expression patterns, and reveals a biphasic activation of genes in surviving tissue. Journal of Neurochemistry. 2006 Jan;96(1):14-29. https://doi.org/10.1111/j.1471-4159.2005.03508.x

Author

Rickhag, Karl Mattias ; Wieloch, Tadeusz ; Gidö, Gunilla ; Elmér, Eskil ; Krogh, Morten ; Murray, Joseph ; Lohr, Scott ; Bitter, Hans ; Chin, Daniel J ; von Schack, David ; Shamloo, Mehrdad ; Nikolich, Karoly. / Comprehensive regional and temporal gene expression profiling of the rat brain during the first 24 h after experimental stroke identifies dynamic ischemia-induced gene expression patterns, and reveals a biphasic activation of genes in surviving tissue. In: Journal of Neurochemistry. 2006 ; Vol. 96, No. 1. pp. 14-29.

Bibtex

@article{f2e98a7c36264ec6bc819aafe9df0352,
title = "Comprehensive regional and temporal gene expression profiling of the rat brain during the first 24 h after experimental stroke identifies dynamic ischemia-induced gene expression patterns, and reveals a biphasic activation of genes in surviving tissue",
abstract = "In order to identify biological processes relevant for cell death and survival in the brain following stroke, the postischemic brain transcriptome was studied by a large-scale cDNA array analysis of three peri-infarct brain regions at eight time points during the first 24 h of reperfusion following middle cerebral artery occlusion in the rat. K-means cluster analysis revealed two distinct biphasic gene expression patterns that contained 44 genes (including 18 immediate early genes), involved in cell signaling and plasticity (i.e. MAP2K7, Sprouty2, Irs-2, Homer1, GPRC5B, Grasp). The first gene induction phase occurred at 0-3 h of reperfusion, and the second at 9-15 h, and was validated by in situ hybridization. Four gene clusters displayed a progressive increase in expression over time and included 50 genes linked to cell motility, lipid synthesis and trafficking (i.e. ApoD, NPC1, G3P-dehydrogenase1, and Choline kinase) or cell death-regulating genes such as mitochondrial CLIC. We conclude that a biphasic transcriptional up-regulation of the brain-derived neurotrophic factor (BDNF)-G-protein coupled receptor (GPCR)-mitogen-activated protein (MAP) kinase signaling pathways occurs in surviving tissue, concomitant with a progressive and persistent activation of cell proliferation signifying tissue regeneration, which provide the means for cell survival and postischemic brain plasticity.",
keywords = "Animals, Autoradiography, Brain, Brain Chemistry, Brain Ischemia, Cell Proliferation, Cell Survival, DNA, Complementary, Gene Expression Profiling, Gene Expression Regulation, In Situ Hybridization, Infarction, Middle Cerebral Artery, Male, Multigene Family, Nerve Regeneration, Neuronal Plasticity, Oligonucleotide Array Sequence Analysis, RNA, Rats, Rats, Wistar, Stroke, Synapses, Transcriptional Activation",
author = "Rickhag, {Karl Mattias} and Tadeusz Wieloch and Gunilla Gid{\"o} and Eskil Elm{\'e}r and Morten Krogh and Joseph Murray and Scott Lohr and Hans Bitter and Chin, {Daniel J} and {von Schack}, David and Mehrdad Shamloo and Karoly Nikolich",
year = "2006",
month = jan,
doi = "10.1111/j.1471-4159.2005.03508.x",
language = "English",
volume = "96",
pages = "14--29",
journal = "Journal of Neurochemistry",
issn = "0022-3042",
publisher = "Wiley-Blackwell",
number = "1",

}

RIS

TY - JOUR

T1 - Comprehensive regional and temporal gene expression profiling of the rat brain during the first 24 h after experimental stroke identifies dynamic ischemia-induced gene expression patterns, and reveals a biphasic activation of genes in surviving tissue

AU - Rickhag, Karl Mattias

AU - Wieloch, Tadeusz

AU - Gidö, Gunilla

AU - Elmér, Eskil

AU - Krogh, Morten

AU - Murray, Joseph

AU - Lohr, Scott

AU - Bitter, Hans

AU - Chin, Daniel J

AU - von Schack, David

AU - Shamloo, Mehrdad

AU - Nikolich, Karoly

PY - 2006/1

Y1 - 2006/1

N2 - In order to identify biological processes relevant for cell death and survival in the brain following stroke, the postischemic brain transcriptome was studied by a large-scale cDNA array analysis of three peri-infarct brain regions at eight time points during the first 24 h of reperfusion following middle cerebral artery occlusion in the rat. K-means cluster analysis revealed two distinct biphasic gene expression patterns that contained 44 genes (including 18 immediate early genes), involved in cell signaling and plasticity (i.e. MAP2K7, Sprouty2, Irs-2, Homer1, GPRC5B, Grasp). The first gene induction phase occurred at 0-3 h of reperfusion, and the second at 9-15 h, and was validated by in situ hybridization. Four gene clusters displayed a progressive increase in expression over time and included 50 genes linked to cell motility, lipid synthesis and trafficking (i.e. ApoD, NPC1, G3P-dehydrogenase1, and Choline kinase) or cell death-regulating genes such as mitochondrial CLIC. We conclude that a biphasic transcriptional up-regulation of the brain-derived neurotrophic factor (BDNF)-G-protein coupled receptor (GPCR)-mitogen-activated protein (MAP) kinase signaling pathways occurs in surviving tissue, concomitant with a progressive and persistent activation of cell proliferation signifying tissue regeneration, which provide the means for cell survival and postischemic brain plasticity.

AB - In order to identify biological processes relevant for cell death and survival in the brain following stroke, the postischemic brain transcriptome was studied by a large-scale cDNA array analysis of three peri-infarct brain regions at eight time points during the first 24 h of reperfusion following middle cerebral artery occlusion in the rat. K-means cluster analysis revealed two distinct biphasic gene expression patterns that contained 44 genes (including 18 immediate early genes), involved in cell signaling and plasticity (i.e. MAP2K7, Sprouty2, Irs-2, Homer1, GPRC5B, Grasp). The first gene induction phase occurred at 0-3 h of reperfusion, and the second at 9-15 h, and was validated by in situ hybridization. Four gene clusters displayed a progressive increase in expression over time and included 50 genes linked to cell motility, lipid synthesis and trafficking (i.e. ApoD, NPC1, G3P-dehydrogenase1, and Choline kinase) or cell death-regulating genes such as mitochondrial CLIC. We conclude that a biphasic transcriptional up-regulation of the brain-derived neurotrophic factor (BDNF)-G-protein coupled receptor (GPCR)-mitogen-activated protein (MAP) kinase signaling pathways occurs in surviving tissue, concomitant with a progressive and persistent activation of cell proliferation signifying tissue regeneration, which provide the means for cell survival and postischemic brain plasticity.

KW - Animals

KW - Autoradiography

KW - Brain

KW - Brain Chemistry

KW - Brain Ischemia

KW - Cell Proliferation

KW - Cell Survival

KW - DNA, Complementary

KW - Gene Expression Profiling

KW - Gene Expression Regulation

KW - In Situ Hybridization

KW - Infarction, Middle Cerebral Artery

KW - Male

KW - Multigene Family

KW - Nerve Regeneration

KW - Neuronal Plasticity

KW - Oligonucleotide Array Sequence Analysis

KW - RNA

KW - Rats

KW - Rats, Wistar

KW - Stroke

KW - Synapses

KW - Transcriptional Activation

U2 - 10.1111/j.1471-4159.2005.03508.x

DO - 10.1111/j.1471-4159.2005.03508.x

M3 - Journal article

C2 - 16300643

VL - 96

SP - 14

EP - 29

JO - Journal of Neurochemistry

JF - Journal of Neurochemistry

SN - 0022-3042

IS - 1

ER -

ID: 132931445