Complexin cooperates with Bruchpilot to tether synaptic vesicles to the active zone cytomatrix

Research output: Contribution to journal › Journal article › Research › peer-review

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Complexin cooperates with Bruchpilot to tether synaptic vesicles to the active zone cytomatrix. / Scholz, Nicole; Ehmann, Nadine; Sachidanandan, Divya; Imig, Cordelia; Cooper, Benjamin H; Jahn, Olaf; Reim, Kerstin; Brose, Nils; Meyer, Jutta; Lamberty, Marius; Altrichter, Steffen; Bormann, Anne; Hallermann, Stefan; Pauli, Martin; Heckmann, Manfred; Stigloher, Christian; Langenhan, Tobias; Kittel, Robert J.

In: The Journal of Cell Biology, Vol. 218, No. 3, 04.03.2019, p. 1011-1026.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Scholz, N, Ehmann, N, Sachidanandan, D, Imig, C, Cooper, BH, Jahn, O, Reim, K, Brose, N, Meyer, J, Lamberty, M, Altrichter, S, Bormann, A, Hallermann, S, Pauli, M, Heckmann, M, Stigloher, C, Langenhan, T & Kittel, RJ 2019, 'Complexin cooperates with Bruchpilot to tether synaptic vesicles to the active zone cytomatrix', The Journal of Cell Biology, vol. 218, no. 3, pp. 1011-1026. https://doi.org/10.1083/jcb.201806155

APA

Scholz, N., Ehmann, N., Sachidanandan, D., Imig, C., Cooper, B. H., Jahn, O., Reim, K., Brose, N., Meyer, J., Lamberty, M., Altrichter, S., Bormann, A., Hallermann, S., Pauli, M., Heckmann, M., Stigloher, C., Langenhan, T., & Kittel, R. J. (2019). Complexin cooperates with Bruchpilot to tether synaptic vesicles to the active zone cytomatrix. The Journal of Cell Biology, 218(3), 1011-1026. https://doi.org/10.1083/jcb.201806155

Vancouver

Scholz N, Ehmann N, Sachidanandan D, Imig C, Cooper BH, Jahn O et al. Complexin cooperates with Bruchpilot to tether synaptic vesicles to the active zone cytomatrix. The Journal of Cell Biology. 2019 Mar 4;218(3):1011-1026. https://doi.org/10.1083/jcb.201806155

Author

Scholz, Nicole ; Ehmann, Nadine ; Sachidanandan, Divya ; Imig, Cordelia ; Cooper, Benjamin H ; Jahn, Olaf ; Reim, Kerstin ; Brose, Nils ; Meyer, Jutta ; Lamberty, Marius ; Altrichter, Steffen ; Bormann, Anne ; Hallermann, Stefan ; Pauli, Martin ; Heckmann, Manfred ; Stigloher, Christian ; Langenhan, Tobias ; Kittel, Robert J. / Complexin cooperates with Bruchpilot to tether synaptic vesicles to the active zone cytomatrix. In: The Journal of Cell Biology. 2019 ; Vol. 218, No. 3. pp. 1011-1026.

Bibtex

@article{326e9f43be7e4fd79500c9a01c0e48ac,
title = "Complexin cooperates with Bruchpilot to tether synaptic vesicles to the active zone cytomatrix",
abstract = "Information processing by the nervous system depends on neurotransmitter release from synaptic vesicles (SVs) at the presynaptic active zone. Molecular components of the cytomatrix at the active zone (CAZ) regulate the final stages of the SV cycle preceding exocytosis and thereby shape the efficacy and plasticity of synaptic transmission. Part of this regulation is reflected by a physical association of SVs with filamentous CAZ structures via largely unknown protein interactions. The very C-terminal region of Bruchpilot (Brp), a key component of the Drosophila melanogaster CAZ, participates in SV tethering. Here, we identify the conserved SNARE regulator Complexin (Cpx) in an in vivo screen for molecules that link the Brp C terminus to SVs. Brp and Cpx interact genetically and functionally. Both proteins promote SV recruitment to the Drosophila CAZ and counteract short-term synaptic depression. Analyzing SV tethering to active zone ribbons of cpx3 knockout mice supports an evolutionarily conserved role of Cpx upstream of SNARE complex assembly.",
keywords = "Adaptor Proteins, Signal Transducing/genetics, Adaptor Proteins, Vesicular Transport/genetics, Animals, Drosophila Proteins/genetics, Drosophila melanogaster, Mice, Mice, Knockout, Nerve Tissue Proteins/genetics, Neuronal Plasticity, Protein Domains, SNARE Proteins/genetics, Synaptic Vesicles/genetics",
author = "Nicole Scholz and Nadine Ehmann and Divya Sachidanandan and Cordelia Imig and Cooper, {Benjamin H} and Olaf Jahn and Kerstin Reim and Nils Brose and Jutta Meyer and Marius Lamberty and Steffen Altrichter and Anne Bormann and Stefan Hallermann and Martin Pauli and Manfred Heckmann and Christian Stigloher and Tobias Langenhan and Kittel, {Robert J}",
note = "{\textcopyright} 2019 Scholz et al.",
year = "2019",
month = mar,
day = "4",
doi = "10.1083/jcb.201806155",
language = "English",
volume = "218",
pages = "1011--1026",
journal = "Journal of Cell Biology",
issn = "0021-9525",
publisher = "Rockefeller University Press",
number = "3",

}

RIS

TY - JOUR

T1 - Complexin cooperates with Bruchpilot to tether synaptic vesicles to the active zone cytomatrix

AU - Scholz, Nicole

AU - Ehmann, Nadine

AU - Sachidanandan, Divya

AU - Imig, Cordelia

AU - Cooper, Benjamin H

AU - Jahn, Olaf

AU - Reim, Kerstin

AU - Brose, Nils

AU - Meyer, Jutta

AU - Lamberty, Marius

AU - Altrichter, Steffen

AU - Bormann, Anne

AU - Hallermann, Stefan

AU - Pauli, Martin

AU - Heckmann, Manfred

AU - Stigloher, Christian

AU - Langenhan, Tobias

AU - Kittel, Robert J

N1 - © 2019 Scholz et al.

PY - 2019/3/4

Y1 - 2019/3/4

N2 - Information processing by the nervous system depends on neurotransmitter release from synaptic vesicles (SVs) at the presynaptic active zone. Molecular components of the cytomatrix at the active zone (CAZ) regulate the final stages of the SV cycle preceding exocytosis and thereby shape the efficacy and plasticity of synaptic transmission. Part of this regulation is reflected by a physical association of SVs with filamentous CAZ structures via largely unknown protein interactions. The very C-terminal region of Bruchpilot (Brp), a key component of the Drosophila melanogaster CAZ, participates in SV tethering. Here, we identify the conserved SNARE regulator Complexin (Cpx) in an in vivo screen for molecules that link the Brp C terminus to SVs. Brp and Cpx interact genetically and functionally. Both proteins promote SV recruitment to the Drosophila CAZ and counteract short-term synaptic depression. Analyzing SV tethering to active zone ribbons of cpx3 knockout mice supports an evolutionarily conserved role of Cpx upstream of SNARE complex assembly.

AB - Information processing by the nervous system depends on neurotransmitter release from synaptic vesicles (SVs) at the presynaptic active zone. Molecular components of the cytomatrix at the active zone (CAZ) regulate the final stages of the SV cycle preceding exocytosis and thereby shape the efficacy and plasticity of synaptic transmission. Part of this regulation is reflected by a physical association of SVs with filamentous CAZ structures via largely unknown protein interactions. The very C-terminal region of Bruchpilot (Brp), a key component of the Drosophila melanogaster CAZ, participates in SV tethering. Here, we identify the conserved SNARE regulator Complexin (Cpx) in an in vivo screen for molecules that link the Brp C terminus to SVs. Brp and Cpx interact genetically and functionally. Both proteins promote SV recruitment to the Drosophila CAZ and counteract short-term synaptic depression. Analyzing SV tethering to active zone ribbons of cpx3 knockout mice supports an evolutionarily conserved role of Cpx upstream of SNARE complex assembly.

KW - Adaptor Proteins, Signal Transducing/genetics

KW - Adaptor Proteins, Vesicular Transport/genetics

KW - Animals

KW - Drosophila Proteins/genetics

KW - Drosophila melanogaster

KW - Mice

KW - Mice, Knockout

KW - Nerve Tissue Proteins/genetics

KW - Neuronal Plasticity

KW - Protein Domains

KW - SNARE Proteins/genetics

KW - Synaptic Vesicles/genetics

U2 - 10.1083/jcb.201806155

DO - 10.1083/jcb.201806155

M3 - Journal article

C2 - 30782781

VL - 218

SP - 1011

EP - 1026

JO - Journal of Cell Biology

JF - Journal of Cell Biology

SN - 0021-9525

IS - 3

ER -

ID: 237697594