Complexin 3 Increases the Fidelity of Signaling in a Retinal Circuit by Regulating Exocytosis at Ribbon Synapses

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Complexin 3 Increases the Fidelity of Signaling in a Retinal Circuit by Regulating Exocytosis at Ribbon Synapses. / Mortensen, Lena S; Park, Silvia J H; Ke, Jiang-Bin; Cooper, Benjamin H; Zhang, Lei; Imig, Cordelia; Löwel, Siegrid; Reim, Kerstin; Brose, Nils; Demb, Jonathan B; Rhee, Jeong-Seop; Singer, Joshua H.

In: Cell Reports, Vol. 15, No. 10, 07.06.2016, p. 2239-2250.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Mortensen, LS, Park, SJH, Ke, J-B, Cooper, BH, Zhang, L, Imig, C, Löwel, S, Reim, K, Brose, N, Demb, JB, Rhee, J-S & Singer, JH 2016, 'Complexin 3 Increases the Fidelity of Signaling in a Retinal Circuit by Regulating Exocytosis at Ribbon Synapses', Cell Reports, vol. 15, no. 10, pp. 2239-2250. https://doi.org/10.1016/j.celrep.2016.05.012

APA

Mortensen, L. S., Park, S. J. H., Ke, J-B., Cooper, B. H., Zhang, L., Imig, C., Löwel, S., Reim, K., Brose, N., Demb, J. B., Rhee, J-S., & Singer, J. H. (2016). Complexin 3 Increases the Fidelity of Signaling in a Retinal Circuit by Regulating Exocytosis at Ribbon Synapses. Cell Reports, 15(10), 2239-2250. https://doi.org/10.1016/j.celrep.2016.05.012

Vancouver

Mortensen LS, Park SJH, Ke J-B, Cooper BH, Zhang L, Imig C et al. Complexin 3 Increases the Fidelity of Signaling in a Retinal Circuit by Regulating Exocytosis at Ribbon Synapses. Cell Reports. 2016 Jun 7;15(10):2239-2250. https://doi.org/10.1016/j.celrep.2016.05.012

Author

Mortensen, Lena S ; Park, Silvia J H ; Ke, Jiang-Bin ; Cooper, Benjamin H ; Zhang, Lei ; Imig, Cordelia ; Löwel, Siegrid ; Reim, Kerstin ; Brose, Nils ; Demb, Jonathan B ; Rhee, Jeong-Seop ; Singer, Joshua H. / Complexin 3 Increases the Fidelity of Signaling in a Retinal Circuit by Regulating Exocytosis at Ribbon Synapses. In: Cell Reports. 2016 ; Vol. 15, No. 10. pp. 2239-2250.

Bibtex

@article{38a5f2a948da4a2b8158833c37684a1f,
title = "Complexin 3 Increases the Fidelity of Signaling in a Retinal Circuit by Regulating Exocytosis at Ribbon Synapses",
abstract = "Complexin (Cplx) proteins modulate the core SNARE complex to regulate exocytosis. To understand the contributions of Cplx to signaling in a well-characterized neural circuit, we investigated how Cplx3, a retina-specific paralog, shapes transmission at rod bipolar (RB)→AII amacrine cell synapses in the mouse retina. Knockout of Cplx3 strongly attenuated fast, phasic Ca(2+)-dependent transmission, dependent on local [Ca(2+)] nanodomains, but enhanced slower Ca(2+)-dependent transmission, dependent on global intraterminal [Ca(2+)] ([Ca(2+)]I). Surprisingly, coordinated multivesicular release persisted at Cplx3(-/-) synapses, although its onset was slowed. Light-dependent signaling at Cplx3(-/-) RB→AII synapses was sluggish, owing largely to increased asynchronous release at light offset. Consequently, propagation of RB output to retinal ganglion cells was suppressed dramatically. Our study links Cplx3 expression with synapse and circuit function in a specific retinal pathway and reveals a role for asynchronous release in circuit gain control.",
keywords = "Animals, Calcium/pharmacology, Exocytosis/drug effects, Eye Proteins/metabolism, Mice, Inbred C57BL, Multivesicular Bodies/drug effects, Nerve Tissue Proteins/deficiency, Retina/cytology, Retinal Bipolar Cells/drug effects, Signal Transduction/drug effects, Synapses/drug effects, Synaptic Transmission/drug effects",
author = "Mortensen, {Lena S} and Park, {Silvia J H} and Jiang-Bin Ke and Cooper, {Benjamin H} and Lei Zhang and Cordelia Imig and Siegrid L{\"o}wel and Kerstin Reim and Nils Brose and Demb, {Jonathan B} and Jeong-Seop Rhee and Singer, {Joshua H}",
note = "Copyright {\textcopyright} 2016 The Author(s). Published by Elsevier Inc. All rights reserved.",
year = "2016",
month = jun,
day = "7",
doi = "10.1016/j.celrep.2016.05.012",
language = "English",
volume = "15",
pages = "2239--2250",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "10",

}

RIS

TY - JOUR

T1 - Complexin 3 Increases the Fidelity of Signaling in a Retinal Circuit by Regulating Exocytosis at Ribbon Synapses

AU - Mortensen, Lena S

AU - Park, Silvia J H

AU - Ke, Jiang-Bin

AU - Cooper, Benjamin H

AU - Zhang, Lei

AU - Imig, Cordelia

AU - Löwel, Siegrid

AU - Reim, Kerstin

AU - Brose, Nils

AU - Demb, Jonathan B

AU - Rhee, Jeong-Seop

AU - Singer, Joshua H

N1 - Copyright © 2016 The Author(s). Published by Elsevier Inc. All rights reserved.

PY - 2016/6/7

Y1 - 2016/6/7

N2 - Complexin (Cplx) proteins modulate the core SNARE complex to regulate exocytosis. To understand the contributions of Cplx to signaling in a well-characterized neural circuit, we investigated how Cplx3, a retina-specific paralog, shapes transmission at rod bipolar (RB)→AII amacrine cell synapses in the mouse retina. Knockout of Cplx3 strongly attenuated fast, phasic Ca(2+)-dependent transmission, dependent on local [Ca(2+)] nanodomains, but enhanced slower Ca(2+)-dependent transmission, dependent on global intraterminal [Ca(2+)] ([Ca(2+)]I). Surprisingly, coordinated multivesicular release persisted at Cplx3(-/-) synapses, although its onset was slowed. Light-dependent signaling at Cplx3(-/-) RB→AII synapses was sluggish, owing largely to increased asynchronous release at light offset. Consequently, propagation of RB output to retinal ganglion cells was suppressed dramatically. Our study links Cplx3 expression with synapse and circuit function in a specific retinal pathway and reveals a role for asynchronous release in circuit gain control.

AB - Complexin (Cplx) proteins modulate the core SNARE complex to regulate exocytosis. To understand the contributions of Cplx to signaling in a well-characterized neural circuit, we investigated how Cplx3, a retina-specific paralog, shapes transmission at rod bipolar (RB)→AII amacrine cell synapses in the mouse retina. Knockout of Cplx3 strongly attenuated fast, phasic Ca(2+)-dependent transmission, dependent on local [Ca(2+)] nanodomains, but enhanced slower Ca(2+)-dependent transmission, dependent on global intraterminal [Ca(2+)] ([Ca(2+)]I). Surprisingly, coordinated multivesicular release persisted at Cplx3(-/-) synapses, although its onset was slowed. Light-dependent signaling at Cplx3(-/-) RB→AII synapses was sluggish, owing largely to increased asynchronous release at light offset. Consequently, propagation of RB output to retinal ganglion cells was suppressed dramatically. Our study links Cplx3 expression with synapse and circuit function in a specific retinal pathway and reveals a role for asynchronous release in circuit gain control.

KW - Animals

KW - Calcium/pharmacology

KW - Exocytosis/drug effects

KW - Eye Proteins/metabolism

KW - Mice, Inbred C57BL

KW - Multivesicular Bodies/drug effects

KW - Nerve Tissue Proteins/deficiency

KW - Retina/cytology

KW - Retinal Bipolar Cells/drug effects

KW - Signal Transduction/drug effects

KW - Synapses/drug effects

KW - Synaptic Transmission/drug effects

U2 - 10.1016/j.celrep.2016.05.012

DO - 10.1016/j.celrep.2016.05.012

M3 - Journal article

C2 - 27239031

VL - 15

SP - 2239

EP - 2250

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 10

ER -

ID: 237697975