Clathrin/AP-2 mediate synaptic vesicle reformation from endosome-like vacuoles but are not essential for membrane retrieval at central synapses

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Clathrin/AP-2 mediate synaptic vesicle reformation from endosome-like vacuoles but are not essential for membrane retrieval at central synapses. / Kononenko, Natalia L; Puchkov, Dmytro; Classen, Gala A; Walter, Alexander M; Pechstein, Arndt; Sawade, Linda; Kaempf, Natalie; Trimbuch, Thorsten; Lorenz, Dorothea; Rosenmund, Christian; Maritzen, Tanja; Haucke, Volker.

In: Neuron, Vol. 82, No. 5, 04.06.2014, p. 981-8.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Kononenko, NL, Puchkov, D, Classen, GA, Walter, AM, Pechstein, A, Sawade, L, Kaempf, N, Trimbuch, T, Lorenz, D, Rosenmund, C, Maritzen, T & Haucke, V 2014, 'Clathrin/AP-2 mediate synaptic vesicle reformation from endosome-like vacuoles but are not essential for membrane retrieval at central synapses', Neuron, vol. 82, no. 5, pp. 981-8. https://doi.org/10.1016/j.neuron.2014.05.007

APA

Kononenko, N. L., Puchkov, D., Classen, G. A., Walter, A. M., Pechstein, A., Sawade, L., Kaempf, N., Trimbuch, T., Lorenz, D., Rosenmund, C., Maritzen, T., & Haucke, V. (2014). Clathrin/AP-2 mediate synaptic vesicle reformation from endosome-like vacuoles but are not essential for membrane retrieval at central synapses. Neuron, 82(5), 981-8. https://doi.org/10.1016/j.neuron.2014.05.007

Vancouver

Kononenko NL, Puchkov D, Classen GA, Walter AM, Pechstein A, Sawade L et al. Clathrin/AP-2 mediate synaptic vesicle reformation from endosome-like vacuoles but are not essential for membrane retrieval at central synapses. Neuron. 2014 Jun 4;82(5):981-8. https://doi.org/10.1016/j.neuron.2014.05.007

Author

Kononenko, Natalia L ; Puchkov, Dmytro ; Classen, Gala A ; Walter, Alexander M ; Pechstein, Arndt ; Sawade, Linda ; Kaempf, Natalie ; Trimbuch, Thorsten ; Lorenz, Dorothea ; Rosenmund, Christian ; Maritzen, Tanja ; Haucke, Volker. / Clathrin/AP-2 mediate synaptic vesicle reformation from endosome-like vacuoles but are not essential for membrane retrieval at central synapses. In: Neuron. 2014 ; Vol. 82, No. 5. pp. 981-8.

Bibtex

@article{d1b59f4c708c45548b46a983c6d665bd,
title = "Clathrin/AP-2 mediate synaptic vesicle reformation from endosome-like vacuoles but are not essential for membrane retrieval at central synapses",
abstract = "Neurotransmission depends on presynaptic membrane retrieval and local reformation of synaptic vesicles (SVs) at nerve terminals. The mechanisms involved in these processes are highly controversial with evidence being presented for SV membranes being retrieved exclusively via clathrin-mediated endocytosis (CME) from the plasma membrane or via ultrafast endocytosis independent of clathrin. Here we show that clathrin and its major adaptor protein 2 (AP-2) in addition to the plasma membrane operate at internal endosome-like vacuoles to regenerate SVs but are not essential for membrane retrieval. Depletion of clathrin or conditional knockout of AP-2 result in defects in SV reformation and an accumulation of endosome-like vacuoles generated by clathrin-independent endocytosis (CIE) via dynamin 1/3 and endophilin. These results together with theoretical modeling provide a conceptual framework for how synapses capitalize on clathrin-independent membrane retrieval and clathrin/AP-2-mediated SV reformation from endosome-like vacuoles to maintain excitability over a broad range of stimulation frequencies.",
keywords = "Adaptor Protein Complex 2/genetics, Animals, Clathrin/genetics, Coated Pits, Cell-Membrane/physiology, Dynamins/metabolism, Endocytosis, Endosomes/physiology, Hippocampus/physiology, Mice, Mice, Inbred C57BL, Mice, Transgenic, Models, Theoretical, Neurons/physiology, Rats, Synapses/physiology, Synaptic Vesicles/physiology",
author = "Kononenko, {Natalia L} and Dmytro Puchkov and Classen, {Gala A} and Walter, {Alexander M} and Arndt Pechstein and Linda Sawade and Natalie Kaempf and Thorsten Trimbuch and Dorothea Lorenz and Christian Rosenmund and Tanja Maritzen and Volker Haucke",
note = "Copyright {\textcopyright} 2014 Elsevier Inc. All rights reserved.",
year = "2014",
month = jun,
day = "4",
doi = "10.1016/j.neuron.2014.05.007",
language = "English",
volume = "82",
pages = "981--8",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "5",

}

RIS

TY - JOUR

T1 - Clathrin/AP-2 mediate synaptic vesicle reformation from endosome-like vacuoles but are not essential for membrane retrieval at central synapses

AU - Kononenko, Natalia L

AU - Puchkov, Dmytro

AU - Classen, Gala A

AU - Walter, Alexander M

AU - Pechstein, Arndt

AU - Sawade, Linda

AU - Kaempf, Natalie

AU - Trimbuch, Thorsten

AU - Lorenz, Dorothea

AU - Rosenmund, Christian

AU - Maritzen, Tanja

AU - Haucke, Volker

N1 - Copyright © 2014 Elsevier Inc. All rights reserved.

PY - 2014/6/4

Y1 - 2014/6/4

N2 - Neurotransmission depends on presynaptic membrane retrieval and local reformation of synaptic vesicles (SVs) at nerve terminals. The mechanisms involved in these processes are highly controversial with evidence being presented for SV membranes being retrieved exclusively via clathrin-mediated endocytosis (CME) from the plasma membrane or via ultrafast endocytosis independent of clathrin. Here we show that clathrin and its major adaptor protein 2 (AP-2) in addition to the plasma membrane operate at internal endosome-like vacuoles to regenerate SVs but are not essential for membrane retrieval. Depletion of clathrin or conditional knockout of AP-2 result in defects in SV reformation and an accumulation of endosome-like vacuoles generated by clathrin-independent endocytosis (CIE) via dynamin 1/3 and endophilin. These results together with theoretical modeling provide a conceptual framework for how synapses capitalize on clathrin-independent membrane retrieval and clathrin/AP-2-mediated SV reformation from endosome-like vacuoles to maintain excitability over a broad range of stimulation frequencies.

AB - Neurotransmission depends on presynaptic membrane retrieval and local reformation of synaptic vesicles (SVs) at nerve terminals. The mechanisms involved in these processes are highly controversial with evidence being presented for SV membranes being retrieved exclusively via clathrin-mediated endocytosis (CME) from the plasma membrane or via ultrafast endocytosis independent of clathrin. Here we show that clathrin and its major adaptor protein 2 (AP-2) in addition to the plasma membrane operate at internal endosome-like vacuoles to regenerate SVs but are not essential for membrane retrieval. Depletion of clathrin or conditional knockout of AP-2 result in defects in SV reformation and an accumulation of endosome-like vacuoles generated by clathrin-independent endocytosis (CIE) via dynamin 1/3 and endophilin. These results together with theoretical modeling provide a conceptual framework for how synapses capitalize on clathrin-independent membrane retrieval and clathrin/AP-2-mediated SV reformation from endosome-like vacuoles to maintain excitability over a broad range of stimulation frequencies.

KW - Adaptor Protein Complex 2/genetics

KW - Animals

KW - Clathrin/genetics

KW - Coated Pits, Cell-Membrane/physiology

KW - Dynamins/metabolism

KW - Endocytosis

KW - Endosomes/physiology

KW - Hippocampus/physiology

KW - Mice

KW - Mice, Inbred C57BL

KW - Mice, Transgenic

KW - Models, Theoretical

KW - Neurons/physiology

KW - Rats

KW - Synapses/physiology

KW - Synaptic Vesicles/physiology

U2 - 10.1016/j.neuron.2014.05.007

DO - 10.1016/j.neuron.2014.05.007

M3 - Journal article

C2 - 24908483

VL - 82

SP - 981

EP - 988

JO - Neuron

JF - Neuron

SN - 0896-6273

IS - 5

ER -

ID: 334036672