Cannabinoid receptor CB2 modulates axon guidance
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Cannabinoid receptor CB2 modulates axon guidance. / Duff, Gabriel; Argaw, Anteneh; Cecyre, Bruno; Cherif, Hosni; Tea, Nicolas; Zabouri, Nawal; Casanova, Christian; Ptito, Maurice; Bouchard, Jean-François.
In: PLOS ONE, Vol. 8, No. 8, 2013, p. e70849.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Cannabinoid receptor CB2 modulates axon guidance
AU - Duff, Gabriel
AU - Argaw, Anteneh
AU - Cecyre, Bruno
AU - Cherif, Hosni
AU - Tea, Nicolas
AU - Zabouri, Nawal
AU - Casanova, Christian
AU - Ptito, Maurice
AU - Bouchard, Jean-François
PY - 2013
Y1 - 2013
N2 - Navigation of retinal projections towards their targets is regulated by guidance molecules and growth cone transduction mechanisms. Here, we present in vitro and in vivo evidences that the cannabinoid receptor 2 (CB2R) is expressed along the retino-thalamic pathway and exerts a modulatory action on axon guidance. These effects are specific to CB2R since no changes were observed in mice where the gene coding for this receptor was altered (cnr2 (-/-)). The CB2R induced morphological changes observed at the growth cone are PKA dependent and require the presence of the netrin-1 receptor, Deleted in Colorectal Cancer. Interfering with endogenous CB2R signalling using pharmacological agents increased retinal axon length and induced aberrant projections. Additionally, cnr2 (-/-) mice showed abnormal eye-specific segregation of retinal projections in the dorsal lateral geniculate nucleus (dLGN) indicating CB2R's implication in retinothalamic development. Overall, this study demonstrates that the contribution of endocannabinoids to brain development is not solely mediated by CB1R, but also involves CB2R.
AB - Navigation of retinal projections towards their targets is regulated by guidance molecules and growth cone transduction mechanisms. Here, we present in vitro and in vivo evidences that the cannabinoid receptor 2 (CB2R) is expressed along the retino-thalamic pathway and exerts a modulatory action on axon guidance. These effects are specific to CB2R since no changes were observed in mice where the gene coding for this receptor was altered (cnr2 (-/-)). The CB2R induced morphological changes observed at the growth cone are PKA dependent and require the presence of the netrin-1 receptor, Deleted in Colorectal Cancer. Interfering with endogenous CB2R signalling using pharmacological agents increased retinal axon length and induced aberrant projections. Additionally, cnr2 (-/-) mice showed abnormal eye-specific segregation of retinal projections in the dorsal lateral geniculate nucleus (dLGN) indicating CB2R's implication in retinothalamic development. Overall, this study demonstrates that the contribution of endocannabinoids to brain development is not solely mediated by CB1R, but also involves CB2R.
KW - Animals
KW - Axons
KW - Cyclic AMP-Dependent Protein Kinases
KW - Embryo, Mammalian
KW - Endocannabinoids
KW - Gene Expression Regulation, Developmental
KW - Geniculate Bodies
KW - Mice
KW - Mice, Knockout
KW - Neurogenesis
KW - Primary Cell Culture
KW - Receptor, Cannabinoid, CB1
KW - Receptor, Cannabinoid, CB2
KW - Receptors, Cell Surface
KW - Retinal Ganglion Cells
KW - Visual Pathways
U2 - 10.1371/journal.pone.0070849
DO - 10.1371/journal.pone.0070849
M3 - Journal article
C2 - 23951024
VL - 8
SP - e70849
JO - PLoS ONE
JF - PLoS ONE
SN - 1932-6203
IS - 8
ER -
ID: 119766040