Bidirectional water fluxes and specificity for small hydrophilic molecules in aquaporins 0-5.

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Standard

Bidirectional water fluxes and specificity for small hydrophilic molecules in aquaporins 0-5. / Meinild, A K; Klærke, Dan Arne; Zeuthen, T.

In: Journal of Biological Chemistry, Vol. 273, No. 49, 1998, p. 32446-51.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Meinild, AK, Klærke, DA & Zeuthen, T 1998, 'Bidirectional water fluxes and specificity for small hydrophilic molecules in aquaporins 0-5.', Journal of Biological Chemistry, vol. 273, no. 49, pp. 32446-51. https://doi.org/10.1074/jbc.273.49.32446

APA

Meinild, A. K., Klærke, D. A., & Zeuthen, T. (1998). Bidirectional water fluxes and specificity for small hydrophilic molecules in aquaporins 0-5. Journal of Biological Chemistry, 273(49), 32446-51. https://doi.org/10.1074/jbc.273.49.32446

Vancouver

Meinild AK, Klærke DA, Zeuthen T. Bidirectional water fluxes and specificity for small hydrophilic molecules in aquaporins 0-5. Journal of Biological Chemistry. 1998;273(49):32446-51. https://doi.org/10.1074/jbc.273.49.32446

Author

Meinild, A K ; Klærke, Dan Arne ; Zeuthen, T. / Bidirectional water fluxes and specificity for small hydrophilic molecules in aquaporins 0-5. In: Journal of Biological Chemistry. 1998 ; Vol. 273, No. 49. pp. 32446-51.

Bibtex

@article{7954df30f1a711dcbee902004c4f4f50,
title = "Bidirectional water fluxes and specificity for small hydrophilic molecules in aquaporins 0-5.",
abstract = "The dimensions of the aqueous pore in aquaporins (AQP) 0, 1, 2, 3, 4, and 5 expressed in Xenopus laevisoocytes were probed by comparing the ability of various solutes to generate osmotic flow. By improved techniques, volume flows were determined from initial rates of changes. Identical values for the osmotic water permeability (L p) were obtained in swelling as in shrinkage experiments demonstrating, for the first time, that aquaporins are bidirectional. The reflection coefficients (¿) of urea, glycerol, acetamide, and formamide at 23¿°C were: AQP0: 1, 1, 0.8, 0.6; AQP1: 1, 0.8, 1, 1; AQP2: 1, 0.8, 1, 1; AQP3: 1, 0.2, 0.7, 0.4; AQP4: 1, 0.9, 1, 1; and AQP5: 1, 1, 1, 0.8. As seen there is no clear connection between solute size and permeation. At 13¿°C the ¿s for AQP3 were 1, 0.4, 1, and 0.5; functionally, this pore narrows at lower temperatures. HgCl2 reversibly reduced theL p of AQP3 and increased ¿glyc to 1 and ¿form to 0.6. We conclude that the pore of the various aquaporins are structurally different and that a simple steric model is insufficient to explain solute-pore interactions.",
author = "Meinild, {A K} and Kl{\ae}rke, {Dan Arne} and T Zeuthen",
note = "Keywords: Animals; Aquaporins; Biological Transport; Humans; Mercuric Chloride; Osmosis; Rats; Water; Xenopus laevis",
year = "1998",
doi = "10.1074/jbc.273.49.32446",
language = "English",
volume = "273",
pages = "32446--51",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology, Inc.",
number = "49",

}

RIS

TY - JOUR

T1 - Bidirectional water fluxes and specificity for small hydrophilic molecules in aquaporins 0-5.

AU - Meinild, A K

AU - Klærke, Dan Arne

AU - Zeuthen, T

N1 - Keywords: Animals; Aquaporins; Biological Transport; Humans; Mercuric Chloride; Osmosis; Rats; Water; Xenopus laevis

PY - 1998

Y1 - 1998

N2 - The dimensions of the aqueous pore in aquaporins (AQP) 0, 1, 2, 3, 4, and 5 expressed in Xenopus laevisoocytes were probed by comparing the ability of various solutes to generate osmotic flow. By improved techniques, volume flows were determined from initial rates of changes. Identical values for the osmotic water permeability (L p) were obtained in swelling as in shrinkage experiments demonstrating, for the first time, that aquaporins are bidirectional. The reflection coefficients (¿) of urea, glycerol, acetamide, and formamide at 23¿°C were: AQP0: 1, 1, 0.8, 0.6; AQP1: 1, 0.8, 1, 1; AQP2: 1, 0.8, 1, 1; AQP3: 1, 0.2, 0.7, 0.4; AQP4: 1, 0.9, 1, 1; and AQP5: 1, 1, 1, 0.8. As seen there is no clear connection between solute size and permeation. At 13¿°C the ¿s for AQP3 were 1, 0.4, 1, and 0.5; functionally, this pore narrows at lower temperatures. HgCl2 reversibly reduced theL p of AQP3 and increased ¿glyc to 1 and ¿form to 0.6. We conclude that the pore of the various aquaporins are structurally different and that a simple steric model is insufficient to explain solute-pore interactions.

AB - The dimensions of the aqueous pore in aquaporins (AQP) 0, 1, 2, 3, 4, and 5 expressed in Xenopus laevisoocytes were probed by comparing the ability of various solutes to generate osmotic flow. By improved techniques, volume flows were determined from initial rates of changes. Identical values for the osmotic water permeability (L p) were obtained in swelling as in shrinkage experiments demonstrating, for the first time, that aquaporins are bidirectional. The reflection coefficients (¿) of urea, glycerol, acetamide, and formamide at 23¿°C were: AQP0: 1, 1, 0.8, 0.6; AQP1: 1, 0.8, 1, 1; AQP2: 1, 0.8, 1, 1; AQP3: 1, 0.2, 0.7, 0.4; AQP4: 1, 0.9, 1, 1; and AQP5: 1, 1, 1, 0.8. As seen there is no clear connection between solute size and permeation. At 13¿°C the ¿s for AQP3 were 1, 0.4, 1, and 0.5; functionally, this pore narrows at lower temperatures. HgCl2 reversibly reduced theL p of AQP3 and increased ¿glyc to 1 and ¿form to 0.6. We conclude that the pore of the various aquaporins are structurally different and that a simple steric model is insufficient to explain solute-pore interactions.

U2 - 10.1074/jbc.273.49.32446

DO - 10.1074/jbc.273.49.32446

M3 - Journal article

C2 - 9829975

VL - 273

SP - 32446

EP - 32451

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 49

ER -

ID: 3153879