BDNF downregulates 5-HT(2A) receptor protein levels in hippocampal cultures

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

BDNF downregulates 5-HT(2A) receptor protein levels in hippocampal cultures. / Trajkovska, V; Santini, M A; Marcussen, Anders Bue; Thomsen, Morten Skøtt; Hansen, H H; Mikkelsen, Jens D.; Arneberg, L; Kokaia, M; Knudsen, G M; Aznar, S.

In: Neurochemistry International, Vol. 55, No. 7, 2009, p. 697-702.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Trajkovska, V, Santini, MA, Marcussen, AB, Thomsen, MS, Hansen, HH, Mikkelsen, JD, Arneberg, L, Kokaia, M, Knudsen, GM & Aznar, S 2009, 'BDNF downregulates 5-HT(2A) receptor protein levels in hippocampal cultures', Neurochemistry International, vol. 55, no. 7, pp. 697-702. https://doi.org/10.1016/j.neuint.2009.06.013

APA

Trajkovska, V., Santini, M. A., Marcussen, A. B., Thomsen, M. S., Hansen, H. H., Mikkelsen, J. D., Arneberg, L., Kokaia, M., Knudsen, G. M., & Aznar, S. (2009). BDNF downregulates 5-HT(2A) receptor protein levels in hippocampal cultures. Neurochemistry International, 55(7), 697-702. https://doi.org/10.1016/j.neuint.2009.06.013

Vancouver

Trajkovska V, Santini MA, Marcussen AB, Thomsen MS, Hansen HH, Mikkelsen JD et al. BDNF downregulates 5-HT(2A) receptor protein levels in hippocampal cultures. Neurochemistry International. 2009;55(7):697-702. https://doi.org/10.1016/j.neuint.2009.06.013

Author

Trajkovska, V ; Santini, M A ; Marcussen, Anders Bue ; Thomsen, Morten Skøtt ; Hansen, H H ; Mikkelsen, Jens D. ; Arneberg, L ; Kokaia, M ; Knudsen, G M ; Aznar, S. / BDNF downregulates 5-HT(2A) receptor protein levels in hippocampal cultures. In: Neurochemistry International. 2009 ; Vol. 55, No. 7. pp. 697-702.

Bibtex

@article{062ead4072df11df928f000ea68e967b,
title = "BDNF downregulates 5-HT(2A) receptor protein levels in hippocampal cultures",
abstract = "Both brain-derived neurotrophic factor (BDNF) and the serotonin receptor 2A (5-HT(2A)) have been related to depression pathology. Specific 5-HT(2A) receptor changes seen in BDNF conditional mutant mice suggest that BDNF regulates the 5-HT(2A) receptor level. Here we show a direct effect of BDNF on 5-HT(2A) receptor protein levels in primary hippocampal neuronal and mature hippocampal organotypic cultures exposed to different BDNF concentrations for either 1, 3, 5 or 7 days. In vivo effects of BDNF on hippocampal 5-HT(2A) receptor levels were further corroborated in (BDNF +/-) mice with reduced BDNF levels. In primary neuronal cultures, 7 days exposure to 25 and 50ng/mL BDNF resulted in downregulation of 5-HT(2A), but not of 5-HT(1A), receptor protein levels. The BDNF-associated downregulation of 5-HT(2A) receptor levels was also observed in mature hippocampal organotypic cultures, excluding confounding effects of BDNF on immature tissue. BDNF +/- mice showed significant increased 5-HT(2A) receptor levels in hippocampus confirming the association between 5-HT(2A) receptor and BDNF levels in vivo. In conclusion, our results point to a regulatory role of BDNF on 5-HT2A receptor levels. This interaction may be an important mechanism in the role of BDNF in affective disorders emphasizing the need for further elucidating the specificity and the mechanism behind this regulation.",
author = "V Trajkovska and Santini, {M A} and Marcussen, {Anders Bue} and Thomsen, {Morten Sk{\o}tt} and Hansen, {H H} and Mikkelsen, {Jens D.} and L Arneberg and M Kokaia and Knudsen, {G M} and S Aznar",
note = "Keywords: Animals; Apoptosis; Blotting, Western; Brain-Derived Neurotrophic Factor; Cells, Cultured; Down-Regulation; Female; Hippocampus; Mice; Mice, Knockout; Nerve Tissue Proteins; Organ Culture Techniques; Pregnancy; Rats; Rats, Sprague-Dawley; Receptor, Serotonin, 5-HT2A; Synapses",
year = "2009",
doi = "10.1016/j.neuint.2009.06.013",
language = "English",
volume = "55",
pages = "697--702",
journal = "Neurochemistry International",
issn = "0197-0186",
publisher = "Elsevier",
number = "7",

}

RIS

TY - JOUR

T1 - BDNF downregulates 5-HT(2A) receptor protein levels in hippocampal cultures

AU - Trajkovska, V

AU - Santini, M A

AU - Marcussen, Anders Bue

AU - Thomsen, Morten Skøtt

AU - Hansen, H H

AU - Mikkelsen, Jens D.

AU - Arneberg, L

AU - Kokaia, M

AU - Knudsen, G M

AU - Aznar, S

N1 - Keywords: Animals; Apoptosis; Blotting, Western; Brain-Derived Neurotrophic Factor; Cells, Cultured; Down-Regulation; Female; Hippocampus; Mice; Mice, Knockout; Nerve Tissue Proteins; Organ Culture Techniques; Pregnancy; Rats; Rats, Sprague-Dawley; Receptor, Serotonin, 5-HT2A; Synapses

PY - 2009

Y1 - 2009

N2 - Both brain-derived neurotrophic factor (BDNF) and the serotonin receptor 2A (5-HT(2A)) have been related to depression pathology. Specific 5-HT(2A) receptor changes seen in BDNF conditional mutant mice suggest that BDNF regulates the 5-HT(2A) receptor level. Here we show a direct effect of BDNF on 5-HT(2A) receptor protein levels in primary hippocampal neuronal and mature hippocampal organotypic cultures exposed to different BDNF concentrations for either 1, 3, 5 or 7 days. In vivo effects of BDNF on hippocampal 5-HT(2A) receptor levels were further corroborated in (BDNF +/-) mice with reduced BDNF levels. In primary neuronal cultures, 7 days exposure to 25 and 50ng/mL BDNF resulted in downregulation of 5-HT(2A), but not of 5-HT(1A), receptor protein levels. The BDNF-associated downregulation of 5-HT(2A) receptor levels was also observed in mature hippocampal organotypic cultures, excluding confounding effects of BDNF on immature tissue. BDNF +/- mice showed significant increased 5-HT(2A) receptor levels in hippocampus confirming the association between 5-HT(2A) receptor and BDNF levels in vivo. In conclusion, our results point to a regulatory role of BDNF on 5-HT2A receptor levels. This interaction may be an important mechanism in the role of BDNF in affective disorders emphasizing the need for further elucidating the specificity and the mechanism behind this regulation.

AB - Both brain-derived neurotrophic factor (BDNF) and the serotonin receptor 2A (5-HT(2A)) have been related to depression pathology. Specific 5-HT(2A) receptor changes seen in BDNF conditional mutant mice suggest that BDNF regulates the 5-HT(2A) receptor level. Here we show a direct effect of BDNF on 5-HT(2A) receptor protein levels in primary hippocampal neuronal and mature hippocampal organotypic cultures exposed to different BDNF concentrations for either 1, 3, 5 or 7 days. In vivo effects of BDNF on hippocampal 5-HT(2A) receptor levels were further corroborated in (BDNF +/-) mice with reduced BDNF levels. In primary neuronal cultures, 7 days exposure to 25 and 50ng/mL BDNF resulted in downregulation of 5-HT(2A), but not of 5-HT(1A), receptor protein levels. The BDNF-associated downregulation of 5-HT(2A) receptor levels was also observed in mature hippocampal organotypic cultures, excluding confounding effects of BDNF on immature tissue. BDNF +/- mice showed significant increased 5-HT(2A) receptor levels in hippocampus confirming the association between 5-HT(2A) receptor and BDNF levels in vivo. In conclusion, our results point to a regulatory role of BDNF on 5-HT2A receptor levels. This interaction may be an important mechanism in the role of BDNF in affective disorders emphasizing the need for further elucidating the specificity and the mechanism behind this regulation.

U2 - 10.1016/j.neuint.2009.06.013

DO - 10.1016/j.neuint.2009.06.013

M3 - Journal article

C2 - 19563850

VL - 55

SP - 697

EP - 702

JO - Neurochemistry International

JF - Neurochemistry International

SN - 0197-0186

IS - 7

ER -

ID: 20196224