An oral Na(V)1.8 blocker improves motor function in mice completely deficient of myelin protein P-0
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An oral Na(V)1.8 blocker improves motor function in mice completely deficient of myelin protein P-0. / Rosberg, Mette R.; Alvarez Herrero, Susana; Krarup, Christian; Moldovan, Mihai.
In: Neuroscience Letters, Vol. 632, 06.10.2016, p. 33-38.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - An oral Na(V)1.8 blocker improves motor function in mice completely deficient of myelin protein P-0
AU - Rosberg, Mette R.
AU - Alvarez Herrero, Susana
AU - Krarup, Christian
AU - Moldovan, Mihai
PY - 2016/10/6
Y1 - 2016/10/6
N2 - Mice deficient of myelin protein P0 are established models of demyelinating Charcot-Marie-Tooth (CMT) disease. Dysmyelination in these mice is associated with an ectopic expression of the sensory neuron specific sodium channel isoform NaV1.8 on motor axons. We reported that in P0+/−, a model of CMT1B, the membrane dysfunction could be acutely improved by a novel oral NaV1.8 blocker referred to as Compound 31 (C31, Bioorg. Med. Chem. Lett. 2010, 20, 6812; AbbVie Inc.). The aim of this study was to investigate the extent to which C31 treatment could also improve the motor axon function in P0-/-, a CMT model with a much more severe neuropathy. We found that the progressive impairment of motor performance from 1 to 4 months of age in P0-/- could be acutely reversed by C31 treatment. The effect was associated with an improvement of the amplitude of the plantar CMAP evoked by tibial nerve stimulation. The corresponding motor nerve excitability studies by “threshold tracking” showed changes after C31 consistent with attenuation of a resting membrane depolarization. Our data suggest that the depolarizing motor conduction failure in P0-/- could be acutely improved by C31. This provides proof-of-concept that treatment with oral subtype-selective NaV1.8 blockers could be used to improve the motor function in severe forms of demyelinating CMT.
AB - Mice deficient of myelin protein P0 are established models of demyelinating Charcot-Marie-Tooth (CMT) disease. Dysmyelination in these mice is associated with an ectopic expression of the sensory neuron specific sodium channel isoform NaV1.8 on motor axons. We reported that in P0+/−, a model of CMT1B, the membrane dysfunction could be acutely improved by a novel oral NaV1.8 blocker referred to as Compound 31 (C31, Bioorg. Med. Chem. Lett. 2010, 20, 6812; AbbVie Inc.). The aim of this study was to investigate the extent to which C31 treatment could also improve the motor axon function in P0-/-, a CMT model with a much more severe neuropathy. We found that the progressive impairment of motor performance from 1 to 4 months of age in P0-/- could be acutely reversed by C31 treatment. The effect was associated with an improvement of the amplitude of the plantar CMAP evoked by tibial nerve stimulation. The corresponding motor nerve excitability studies by “threshold tracking” showed changes after C31 consistent with attenuation of a resting membrane depolarization. Our data suggest that the depolarizing motor conduction failure in P0-/- could be acutely improved by C31. This provides proof-of-concept that treatment with oral subtype-selective NaV1.8 blockers could be used to improve the motor function in severe forms of demyelinating CMT.
KW - Voltage-gated sodium channels
KW - Charcot-Marie-Tooth disease
KW - Nerve excitability
KW - Myelin protein P-0
KW - Conduction failure
U2 - 10.1016/j.neulet.2016.08.019
DO - 10.1016/j.neulet.2016.08.019
M3 - Journal article
C2 - 27530546
VL - 632
SP - 33
EP - 38
JO - Neuroscience letters. Supplement
JF - Neuroscience letters. Supplement
SN - 0167-6253
ER -
ID: 169565620