An Improved Method for Growing Primary Neurons on Electron Microscopy Grids Co-Cultured with Astrocytes
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With the increasing popularity of cryo-electron tomography (cryo-ET) in recent years, the quest to establish a method for growing primary neurons directly on electron microscopy grids (EM grids) has been ongoing. Here we describe a straightforward way to establish a mature neuronal network on EM grids, which includes formation of synaptic contacts. These synapses were thin enough to allow for direct visualization of small filaments such as SNARE proteins tethering the synaptic vesicle (SV) to the active zone plasma membrane on a Titan Krios without prior focused ion-beam milling.
Original language | English |
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Article number | 15191 |
Journal | International Journal of Molecular Sciences |
Volume | 24 |
Issue number | 20 |
Number of pages | 15 |
ISSN | 1661-6596 |
DOIs | |
Publication status | Published - 2023 |
Bibliographical note
Publisher Copyright:
© 2023 by the authors.
- astrocytes, cryo-electron microscopy, primary neurons, synapse
Research areas
ID: 372327571