An active vesicle priming machinery suppresses axon regeneration upon adult CNS injury

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

An active vesicle priming machinery suppresses axon regeneration upon adult CNS injury. / Hilton, Brett J; Husch, Andreas; Schaffran, Barbara; Lin, Tien-Chen; Burnside, Emily R; Dupraz, Sebastian; Schelski, Max; Kim, Jisoo; Müller, Johannes Alexander; Schoch, Susanne; Imig, Cordelia; Brose, Nils; Bradke, Frank.

In: Neuron, Vol. 110, 19.10.2021.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Hilton, BJ, Husch, A, Schaffran, B, Lin, T-C, Burnside, ER, Dupraz, S, Schelski, M, Kim, J, Müller, JA, Schoch, S, Imig, C, Brose, N & Bradke, F 2021, 'An active vesicle priming machinery suppresses axon regeneration upon adult CNS injury', Neuron, vol. 110. https://doi.org/10.1016/j.neuron.2021.10.007

APA

Hilton, B. J., Husch, A., Schaffran, B., Lin, T-C., Burnside, E. R., Dupraz, S., Schelski, M., Kim, J., Müller, J. A., Schoch, S., Imig, C., Brose, N., & Bradke, F. (2021). An active vesicle priming machinery suppresses axon regeneration upon adult CNS injury. Neuron, 110. https://doi.org/10.1016/j.neuron.2021.10.007

Vancouver

Hilton BJ, Husch A, Schaffran B, Lin T-C, Burnside ER, Dupraz S et al. An active vesicle priming machinery suppresses axon regeneration upon adult CNS injury. Neuron. 2021 Oct 19;110. https://doi.org/10.1016/j.neuron.2021.10.007

Author

Hilton, Brett J ; Husch, Andreas ; Schaffran, Barbara ; Lin, Tien-Chen ; Burnside, Emily R ; Dupraz, Sebastian ; Schelski, Max ; Kim, Jisoo ; Müller, Johannes Alexander ; Schoch, Susanne ; Imig, Cordelia ; Brose, Nils ; Bradke, Frank. / An active vesicle priming machinery suppresses axon regeneration upon adult CNS injury. In: Neuron. 2021 ; Vol. 110.

Bibtex

@article{7206b0d1dee94ef183182b759b26c1fc,

title = "An active vesicle priming machinery suppresses axon regeneration upon adult CNS injury",

abstract = "Axons in the adult mammalian central nervous system fail to regenerate after spinal cord injury. Neurons lose their capacity to regenerate during development, but the intracellular processes underlying this loss are unclear. We found that critical components of the presynaptic active zone prevent axon regeneration in adult mice. Transcriptomic analysis combined with live-cell imaging revealed that adult primary sensory neurons downregulate molecular constituents of the synapse as they acquire the ability to rapidly grow their axons. Pharmacogenetic reduction of neuronal excitability stimulated axon regeneration after adult spinal cord injury. Genetic gain- and loss-of-function experiments uncovered that essential synaptic vesicle priming proteins of the presynaptic active zone, but not clostridial-toxin-sensitive VAMP-family SNARE proteins, inhibit axon regeneration. Systemic administration of Baclofen reduced voltage-dependent Ca2+ influx in primary sensory neurons and promoted their regeneration after spinal cord injury. These findings indicate that functional presynaptic active zones constitute a major barrier to axon regeneration.",

author = "Hilton, {Brett J} and Andreas Husch and Barbara Schaffran and Tien-Chen Lin and Burnside, {Emily R} and Sebastian Dupraz and Max Schelski and Jisoo Kim and M{\"u}ller, {Johannes Alexander} and Susanne Schoch and Cordelia Imig and Nils Brose and Frank Bradke",

year = "2021",

month = oct,

day = "19",

doi = "10.1016/j.neuron.2021.10.007",

language = "English",

volume = "110",

journal = "Neuron",

issn = "0896-6273",

publisher = "Cell Press",

}

RIS

TY - JOUR

T1 - An active vesicle priming machinery suppresses axon regeneration upon adult CNS injury

AU - Hilton, Brett J

AU - Husch, Andreas

AU - Schaffran, Barbara

AU - Lin, Tien-Chen

AU - Burnside, Emily R

AU - Dupraz, Sebastian

AU - Schelski, Max

AU - Kim, Jisoo

AU - Müller, Johannes Alexander

AU - Schoch, Susanne

AU - Imig, Cordelia

AU - Brose, Nils

AU - Bradke, Frank

PY - 2021/10/19

Y1 - 2021/10/19

N2 - Axons in the adult mammalian central nervous system fail to regenerate after spinal cord injury. Neurons lose their capacity to regenerate during development, but the intracellular processes underlying this loss are unclear. We found that critical components of the presynaptic active zone prevent axon regeneration in adult mice. Transcriptomic analysis combined with live-cell imaging revealed that adult primary sensory neurons downregulate molecular constituents of the synapse as they acquire the ability to rapidly grow their axons. Pharmacogenetic reduction of neuronal excitability stimulated axon regeneration after adult spinal cord injury. Genetic gain- and loss-of-function experiments uncovered that essential synaptic vesicle priming proteins of the presynaptic active zone, but not clostridial-toxin-sensitive VAMP-family SNARE proteins, inhibit axon regeneration. Systemic administration of Baclofen reduced voltage-dependent Ca2+ influx in primary sensory neurons and promoted their regeneration after spinal cord injury. These findings indicate that functional presynaptic active zones constitute a major barrier to axon regeneration.

AB - Axons in the adult mammalian central nervous system fail to regenerate after spinal cord injury. Neurons lose their capacity to regenerate during development, but the intracellular processes underlying this loss are unclear. We found that critical components of the presynaptic active zone prevent axon regeneration in adult mice. Transcriptomic analysis combined with live-cell imaging revealed that adult primary sensory neurons downregulate molecular constituents of the synapse as they acquire the ability to rapidly grow their axons. Pharmacogenetic reduction of neuronal excitability stimulated axon regeneration after adult spinal cord injury. Genetic gain- and loss-of-function experiments uncovered that essential synaptic vesicle priming proteins of the presynaptic active zone, but not clostridial-toxin-sensitive VAMP-family SNARE proteins, inhibit axon regeneration. Systemic administration of Baclofen reduced voltage-dependent Ca2+ influx in primary sensory neurons and promoted their regeneration after spinal cord injury. These findings indicate that functional presynaptic active zones constitute a major barrier to axon regeneration.

U2 - 10.1016/j.neuron.2021.10.007

DO - 10.1016/j.neuron.2021.10.007

M3 - Journal article

C2 - 34706221

VL - 110

JO - Neuron

JF - Neuron

SN - 0896-6273

ER -

ID: 282873544

Department of Neuroscience