Additive effects on the energy barrier for synaptic vesicle fusion cause supralinear effects on the vesicle fusion rate

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Additive effects on the energy barrier for synaptic vesicle fusion cause supralinear effects on the vesicle fusion rate. / Schotten, Sebastiaan; Meijer, Marieke; Walter, Alexander Matthias; Huson, Vincent; Mamer, Lauren; Kalogreades, Lawrence; Ter Veer, Mirelle; Ruiter, Marvin; Brose, Nils; Rosenmund, Christian; Sørensen, Jakob Balslev; Verhage, Matthijs; Cornelisse, Lennart Niels.

In: eLife, Vol. 4, e05531, 2015, p. 1-25.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Schotten, S, Meijer, M, Walter, AM, Huson, V, Mamer, L, Kalogreades, L, Ter Veer, M, Ruiter, M, Brose, N, Rosenmund, C, Sørensen, JB, Verhage, M & Cornelisse, LN 2015, 'Additive effects on the energy barrier for synaptic vesicle fusion cause supralinear effects on the vesicle fusion rate', eLife, vol. 4, e05531, pp. 1-25. https://doi.org/10.7554/eLife.05531

APA

Schotten, S., Meijer, M., Walter, A. M., Huson, V., Mamer, L., Kalogreades, L., Ter Veer, M., Ruiter, M., Brose, N., Rosenmund, C., Sørensen, J. B., Verhage, M., & Cornelisse, L. N. (2015). Additive effects on the energy barrier for synaptic vesicle fusion cause supralinear effects on the vesicle fusion rate. eLife, 4, 1-25. [e05531]. https://doi.org/10.7554/eLife.05531

Vancouver

Schotten S, Meijer M, Walter AM, Huson V, Mamer L, Kalogreades L et al. Additive effects on the energy barrier for synaptic vesicle fusion cause supralinear effects on the vesicle fusion rate. eLife. 2015;4:1-25. e05531. https://doi.org/10.7554/eLife.05531

Author

Schotten, Sebastiaan ; Meijer, Marieke ; Walter, Alexander Matthias ; Huson, Vincent ; Mamer, Lauren ; Kalogreades, Lawrence ; Ter Veer, Mirelle ; Ruiter, Marvin ; Brose, Nils ; Rosenmund, Christian ; Sørensen, Jakob Balslev ; Verhage, Matthijs ; Cornelisse, Lennart Niels. / Additive effects on the energy barrier for synaptic vesicle fusion cause supralinear effects on the vesicle fusion rate. In: eLife. 2015 ; Vol. 4. pp. 1-25.

Bibtex

@article{323d0fa048604d4584b003d34b22f4d0,
title = "Additive effects on the energy barrier for synaptic vesicle fusion cause supralinear effects on the vesicle fusion rate",
abstract = "The energy required to fuse synaptic vesicles with the plasma membrane ({\textquoteleft}activation energy{\textquoteright}) is considered a major determinant in synaptic efficacy. From reaction rate theory, we predict that a class of modulations exists, which utilize linear modulation of the energy barrier for fusion to achieve supralinear effects on the fusion rate. To test this prediction experimentally, we developed a method to assess the number of releasable vesicles, rate constants for vesicle priming, unpriming, and fusion, and the activation energy for fusion by fitting a vesicle state model to synaptic responses induced by hypertonic solutions. We show that complexinI/II deficiency or phorbol ester stimulation indeed affects responses to hypertonic solution in a supralinear manner. An additive vs multiplicative relationship between activation energy and fusion rate provides a novel explanation for previously observed non-linear effects of genetic/pharmacological perturbations on synaptic transmission and a novel interpretation of the cooperative nature of Ca2+-dependent release.",
author = "Sebastiaan Schotten and Marieke Meijer and Walter, {Alexander Matthias} and Vincent Huson and Lauren Mamer and Lawrence Kalogreades and {Ter Veer}, Mirelle and Marvin Ruiter and Nils Brose and Christian Rosenmund and S{\o}rensen, {Jakob Balslev} and Matthijs Verhage and Cornelisse, {Lennart Niels}",
year = "2015",
doi = "10.7554/eLife.05531",
language = "English",
volume = "4",
pages = "1--25",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications Ltd.",

}

RIS

TY - JOUR

T1 - Additive effects on the energy barrier for synaptic vesicle fusion cause supralinear effects on the vesicle fusion rate

AU - Schotten, Sebastiaan

AU - Meijer, Marieke

AU - Walter, Alexander Matthias

AU - Huson, Vincent

AU - Mamer, Lauren

AU - Kalogreades, Lawrence

AU - Ter Veer, Mirelle

AU - Ruiter, Marvin

AU - Brose, Nils

AU - Rosenmund, Christian

AU - Sørensen, Jakob Balslev

AU - Verhage, Matthijs

AU - Cornelisse, Lennart Niels

PY - 2015

Y1 - 2015

N2 - The energy required to fuse synaptic vesicles with the plasma membrane (‘activation energy’) is considered a major determinant in synaptic efficacy. From reaction rate theory, we predict that a class of modulations exists, which utilize linear modulation of the energy barrier for fusion to achieve supralinear effects on the fusion rate. To test this prediction experimentally, we developed a method to assess the number of releasable vesicles, rate constants for vesicle priming, unpriming, and fusion, and the activation energy for fusion by fitting a vesicle state model to synaptic responses induced by hypertonic solutions. We show that complexinI/II deficiency or phorbol ester stimulation indeed affects responses to hypertonic solution in a supralinear manner. An additive vs multiplicative relationship between activation energy and fusion rate provides a novel explanation for previously observed non-linear effects of genetic/pharmacological perturbations on synaptic transmission and a novel interpretation of the cooperative nature of Ca2+-dependent release.

AB - The energy required to fuse synaptic vesicles with the plasma membrane (‘activation energy’) is considered a major determinant in synaptic efficacy. From reaction rate theory, we predict that a class of modulations exists, which utilize linear modulation of the energy barrier for fusion to achieve supralinear effects on the fusion rate. To test this prediction experimentally, we developed a method to assess the number of releasable vesicles, rate constants for vesicle priming, unpriming, and fusion, and the activation energy for fusion by fitting a vesicle state model to synaptic responses induced by hypertonic solutions. We show that complexinI/II deficiency or phorbol ester stimulation indeed affects responses to hypertonic solution in a supralinear manner. An additive vs multiplicative relationship between activation energy and fusion rate provides a novel explanation for previously observed non-linear effects of genetic/pharmacological perturbations on synaptic transmission and a novel interpretation of the cooperative nature of Ca2+-dependent release.

U2 - 10.7554/eLife.05531

DO - 10.7554/eLife.05531

M3 - Journal article

C2 - 25871846

VL - 4

SP - 1

EP - 25

JO - eLife

JF - eLife

SN - 2050-084X

M1 - e05531

ER -

ID: 137165356