Active Zone Scaffold Protein Ratios Tune Functional Diversity across Brain Synapses

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Active Zone Scaffold Protein Ratios Tune Functional Diversity across Brain Synapses. / Fulterer, Andreas; Andlauer, Till F M; Ender, Anatoli; Maglione, Marta; Eyring, Katherine; Woitkuhn, Jennifer; Lehmann, Martin; Matkovic-Rachid, Tanja; Geiger, Joerg R P; Walter, Alexander M; Nagel, Katherine I; Sigrist, Stephan J.

In: Cell Reports, Vol. 23, No. 5, 01.05.2018, p. 1259-1274.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Fulterer, A, Andlauer, TFM, Ender, A, Maglione, M, Eyring, K, Woitkuhn, J, Lehmann, M, Matkovic-Rachid, T, Geiger, JRP, Walter, AM, Nagel, KI & Sigrist, SJ 2018, 'Active Zone Scaffold Protein Ratios Tune Functional Diversity across Brain Synapses', Cell Reports, vol. 23, no. 5, pp. 1259-1274. https://doi.org/10.1016/j.celrep.2018.03.126

APA

Fulterer, A., Andlauer, T. F. M., Ender, A., Maglione, M., Eyring, K., Woitkuhn, J., Lehmann, M., Matkovic-Rachid, T., Geiger, J. R. P., Walter, A. M., Nagel, K. I., & Sigrist, S. J. (2018). Active Zone Scaffold Protein Ratios Tune Functional Diversity across Brain Synapses. Cell Reports, 23(5), 1259-1274. https://doi.org/10.1016/j.celrep.2018.03.126

Vancouver

Fulterer A, Andlauer TFM, Ender A, Maglione M, Eyring K, Woitkuhn J et al. Active Zone Scaffold Protein Ratios Tune Functional Diversity across Brain Synapses. Cell Reports. 2018 May 1;23(5):1259-1274. https://doi.org/10.1016/j.celrep.2018.03.126

Author

Fulterer, Andreas ; Andlauer, Till F M ; Ender, Anatoli ; Maglione, Marta ; Eyring, Katherine ; Woitkuhn, Jennifer ; Lehmann, Martin ; Matkovic-Rachid, Tanja ; Geiger, Joerg R P ; Walter, Alexander M ; Nagel, Katherine I ; Sigrist, Stephan J. / Active Zone Scaffold Protein Ratios Tune Functional Diversity across Brain Synapses. In: Cell Reports. 2018 ; Vol. 23, No. 5. pp. 1259-1274.

Bibtex

@article{83916d8a5fa04eb39f6e18eeadbc0814,
title = "Active Zone Scaffold Protein Ratios Tune Functional Diversity across Brain Synapses",
abstract = "High-throughput electron microscopy has started to reveal synaptic connectivity maps of single circuits and whole brain regions, for example, in the Drosophila olfactory system. However, efficacy, timing, and frequency tuning of synaptic vesicle release are also highly diversified across brain synapses. These features critically depend on the nanometer-scale coupling distance between voltage-gated Ca2+ channels (VGCCs) and the synaptic vesicle release machinery. Combining light super resolution microscopy with in vivo electrophysiology, we show here that two orthogonal scaffold proteins (ELKS family Bruchpilot, BRP, and Syd-1) cluster-specific (M)Unc13 release factor isoforms either close (BRP/Unc13A) or further away (Syd-1/Unc13B) from VGCCs across synapses of the Drosophila olfactory system, resulting in different synapse-characteristic forms of short-term plasticity. Moreover, BRP/Unc13A versus Syd-1/Unc13B ratios were different between synapse types. Thus, variation in tightly versus loosely coupled scaffold protein/(M)Unc13 modules can tune synapse-type-specific release features, and {"}nanoscopic molecular fingerprints{"} might identify synapses with specific temporal features.",
keywords = "Animals, Drosophila Proteins/metabolism, Drosophila melanogaster, GTPase-Activating Proteins/metabolism, Membrane Proteins/metabolism, Mushroom Bodies/metabolism, Nerve Tissue Proteins/metabolism, Synaptic Vesicles/metabolism",
author = "Andreas Fulterer and Andlauer, {Till F M} and Anatoli Ender and Marta Maglione and Katherine Eyring and Jennifer Woitkuhn and Martin Lehmann and Tanja Matkovic-Rachid and Geiger, {Joerg R P} and Walter, {Alexander M} and Nagel, {Katherine I} and Sigrist, {Stephan J}",
note = "Copyright {\textcopyright} 2018 The Author(s). Published by Elsevier Inc. All rights reserved.",
year = "2018",
month = may,
day = "1",
doi = "10.1016/j.celrep.2018.03.126",
language = "English",
volume = "23",
pages = "1259--1274",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "5",

}

RIS

TY - JOUR

T1 - Active Zone Scaffold Protein Ratios Tune Functional Diversity across Brain Synapses

AU - Fulterer, Andreas

AU - Andlauer, Till F M

AU - Ender, Anatoli

AU - Maglione, Marta

AU - Eyring, Katherine

AU - Woitkuhn, Jennifer

AU - Lehmann, Martin

AU - Matkovic-Rachid, Tanja

AU - Geiger, Joerg R P

AU - Walter, Alexander M

AU - Nagel, Katherine I

AU - Sigrist, Stephan J

N1 - Copyright © 2018 The Author(s). Published by Elsevier Inc. All rights reserved.

PY - 2018/5/1

Y1 - 2018/5/1

N2 - High-throughput electron microscopy has started to reveal synaptic connectivity maps of single circuits and whole brain regions, for example, in the Drosophila olfactory system. However, efficacy, timing, and frequency tuning of synaptic vesicle release are also highly diversified across brain synapses. These features critically depend on the nanometer-scale coupling distance between voltage-gated Ca2+ channels (VGCCs) and the synaptic vesicle release machinery. Combining light super resolution microscopy with in vivo electrophysiology, we show here that two orthogonal scaffold proteins (ELKS family Bruchpilot, BRP, and Syd-1) cluster-specific (M)Unc13 release factor isoforms either close (BRP/Unc13A) or further away (Syd-1/Unc13B) from VGCCs across synapses of the Drosophila olfactory system, resulting in different synapse-characteristic forms of short-term plasticity. Moreover, BRP/Unc13A versus Syd-1/Unc13B ratios were different between synapse types. Thus, variation in tightly versus loosely coupled scaffold protein/(M)Unc13 modules can tune synapse-type-specific release features, and "nanoscopic molecular fingerprints" might identify synapses with specific temporal features.

AB - High-throughput electron microscopy has started to reveal synaptic connectivity maps of single circuits and whole brain regions, for example, in the Drosophila olfactory system. However, efficacy, timing, and frequency tuning of synaptic vesicle release are also highly diversified across brain synapses. These features critically depend on the nanometer-scale coupling distance between voltage-gated Ca2+ channels (VGCCs) and the synaptic vesicle release machinery. Combining light super resolution microscopy with in vivo electrophysiology, we show here that two orthogonal scaffold proteins (ELKS family Bruchpilot, BRP, and Syd-1) cluster-specific (M)Unc13 release factor isoforms either close (BRP/Unc13A) or further away (Syd-1/Unc13B) from VGCCs across synapses of the Drosophila olfactory system, resulting in different synapse-characteristic forms of short-term plasticity. Moreover, BRP/Unc13A versus Syd-1/Unc13B ratios were different between synapse types. Thus, variation in tightly versus loosely coupled scaffold protein/(M)Unc13 modules can tune synapse-type-specific release features, and "nanoscopic molecular fingerprints" might identify synapses with specific temporal features.

KW - Animals

KW - Drosophila Proteins/metabolism

KW - Drosophila melanogaster

KW - GTPase-Activating Proteins/metabolism

KW - Membrane Proteins/metabolism

KW - Mushroom Bodies/metabolism

KW - Nerve Tissue Proteins/metabolism

KW - Synaptic Vesicles/metabolism

U2 - 10.1016/j.celrep.2018.03.126

DO - 10.1016/j.celrep.2018.03.126

M3 - Journal article

C2 - 29719243

VL - 23

SP - 1259

EP - 1274

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 5

ER -

ID: 334034215