A presynaptic role for the cytomatrix protein GIT in synaptic vesicle recycling

Research output: Contribution to journal › Journal article › Research › peer-review

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A presynaptic role for the cytomatrix protein GIT in synaptic vesicle recycling. / Podufall, Jasmin; Tian, Rui; Knoche, Elena; Puchkov, Dmytro; Walter, Alexander M.; Rosa, Stefanie; Quentin, Christine; Vukoja, Anela; Jung, Nadja; Lampe, Andre; Wichmann, Carolin; Böhme, Mathias; Depner, Harald; Zhang, Yong Q.; Schmoranzer, Jan; Sigrist, Stephan J; Haucke, Volker.

In: Cell Reports, Vol. 7, No. 5, 2014, p. 1417-1425.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Podufall, J, Tian, R, Knoche, E, Puchkov, D, Walter, AM, Rosa, S, Quentin, C, Vukoja, A, Jung, N, Lampe, A, Wichmann, C, Böhme, M, Depner, H, Zhang, YQ, Schmoranzer, J, Sigrist, SJ & Haucke, V 2014, 'A presynaptic role for the cytomatrix protein GIT in synaptic vesicle recycling', Cell Reports, vol. 7, no. 5, pp. 1417-1425. https://doi.org/10.1016/j.celrep.2014.04.051

APA

Podufall, J., Tian, R., Knoche, E., Puchkov, D., Walter, A. M., Rosa, S., Quentin, C., Vukoja, A., Jung, N., Lampe, A., Wichmann, C., Böhme, M., Depner, H., Zhang, Y. Q., Schmoranzer, J., Sigrist, S. J., & Haucke, V. (2014). A presynaptic role for the cytomatrix protein GIT in synaptic vesicle recycling. Cell Reports, 7(5), 1417-1425. https://doi.org/10.1016/j.celrep.2014.04.051

Vancouver

Podufall J, Tian R, Knoche E, Puchkov D, Walter AM, Rosa S et al. A presynaptic role for the cytomatrix protein GIT in synaptic vesicle recycling. Cell Reports. 2014;7(5):1417-1425. https://doi.org/10.1016/j.celrep.2014.04.051

Author

Podufall, Jasmin ; Tian, Rui ; Knoche, Elena ; Puchkov, Dmytro ; Walter, Alexander M. ; Rosa, Stefanie ; Quentin, Christine ; Vukoja, Anela ; Jung, Nadja ; Lampe, Andre ; Wichmann, Carolin ; Böhme, Mathias ; Depner, Harald ; Zhang, Yong Q. ; Schmoranzer, Jan ; Sigrist, Stephan J ; Haucke, Volker. / A presynaptic role for the cytomatrix protein GIT in synaptic vesicle recycling. In: Cell Reports. 2014 ; Vol. 7, No. 5. pp. 1417-1425.

Bibtex

@article{72dbf4410e5347609a6d8572b2cb165f,
title = "A presynaptic role for the cytomatrix protein GIT in synaptic vesicle recycling",
abstract = "Neurotransmission involves the exo-endocytic cycling of synaptic vesicles (SVs) within nerve terminals. Exocytosis is facilitated by a cytomatrix assembled at the active zone (AZ). The precise spatial and functional relationship between exocytic fusion of SVs at AZ membranes and endocytic SV retrieval is unknown. Here, we identify the scaffold G protein coupled receptor kinase 2 interacting (GIT) protein as a component of the AZ-associated cytomatrix and as a regulator of SV endocytosis. GIT1 and its D. melanogaster ortholog, dGIT, are shown to directly associate with the endocytic adaptor stonin 2/stoned B. In Drosophila dgit mutants, stoned B and synaptotagmin levels are reduced and stoned B is partially mislocalized. Moreover, dgit mutants show morphological and functional defects in SV recycling. These data establish a presynaptic role for GIT in SV recycling and suggest a connection between the AZ cytomatrix and the endocytic machinery.",
author = "Jasmin Podufall and Rui Tian and Elena Knoche and Dmytro Puchkov and Walter, {Alexander M.} and Stefanie Rosa and Christine Quentin and Anela Vukoja and Nadja Jung and Andre Lampe and Carolin Wichmann and Mathias B{\"o}hme and Harald Depner and Zhang, {Yong Q.} and Jan Schmoranzer and Sigrist, {Stephan J} and Volker Haucke",
year = "2014",
doi = "10.1016/j.celrep.2014.04.051",
language = "English",
volume = "7",
pages = "1417--1425",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "5",

}

RIS

TY - JOUR

T1 - A presynaptic role for the cytomatrix protein GIT in synaptic vesicle recycling

AU - Podufall, Jasmin

AU - Tian, Rui

AU - Knoche, Elena

AU - Puchkov, Dmytro

AU - Walter, Alexander M.

AU - Rosa, Stefanie

AU - Quentin, Christine

AU - Vukoja, Anela

AU - Jung, Nadja

AU - Lampe, Andre

AU - Wichmann, Carolin

AU - Böhme, Mathias

AU - Depner, Harald

AU - Zhang, Yong Q.

AU - Schmoranzer, Jan

AU - Sigrist, Stephan J

AU - Haucke, Volker

PY - 2014

Y1 - 2014

N2 - Neurotransmission involves the exo-endocytic cycling of synaptic vesicles (SVs) within nerve terminals. Exocytosis is facilitated by a cytomatrix assembled at the active zone (AZ). The precise spatial and functional relationship between exocytic fusion of SVs at AZ membranes and endocytic SV retrieval is unknown. Here, we identify the scaffold G protein coupled receptor kinase 2 interacting (GIT) protein as a component of the AZ-associated cytomatrix and as a regulator of SV endocytosis. GIT1 and its D. melanogaster ortholog, dGIT, are shown to directly associate with the endocytic adaptor stonin 2/stoned B. In Drosophila dgit mutants, stoned B and synaptotagmin levels are reduced and stoned B is partially mislocalized. Moreover, dgit mutants show morphological and functional defects in SV recycling. These data establish a presynaptic role for GIT in SV recycling and suggest a connection between the AZ cytomatrix and the endocytic machinery.

AB - Neurotransmission involves the exo-endocytic cycling of synaptic vesicles (SVs) within nerve terminals. Exocytosis is facilitated by a cytomatrix assembled at the active zone (AZ). The precise spatial and functional relationship between exocytic fusion of SVs at AZ membranes and endocytic SV retrieval is unknown. Here, we identify the scaffold G protein coupled receptor kinase 2 interacting (GIT) protein as a component of the AZ-associated cytomatrix and as a regulator of SV endocytosis. GIT1 and its D. melanogaster ortholog, dGIT, are shown to directly associate with the endocytic adaptor stonin 2/stoned B. In Drosophila dgit mutants, stoned B and synaptotagmin levels are reduced and stoned B is partially mislocalized. Moreover, dgit mutants show morphological and functional defects in SV recycling. These data establish a presynaptic role for GIT in SV recycling and suggest a connection between the AZ cytomatrix and the endocytic machinery.

U2 - 10.1016/j.celrep.2014.04.051

DO - 10.1016/j.celrep.2014.04.051

M3 - Journal article

C2 - 24882013

VL - 7

SP - 1417

EP - 1425

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 5

ER -

ID: 334036352