A monoclonal antibody for G protein-coupled receptor crystallography

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

A monoclonal antibody for G protein-coupled receptor crystallography. / Day, Peter W; Rasmussen, Søren Gøgsig Faarup; Parnot, Charles; Fung, Juan José; Masood, Asna; Kobilka, Tong Sun; Yao, Xiao-Jie; Choi, Hee-Jung; Weis, William I; Rohrer, Daniel K; Kobilka, Brian K.

In: Nature Methods, Vol. 4, No. 11, 11.2007, p. 927-9.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Day, PW, Rasmussen, SGF, Parnot, C, Fung, JJ, Masood, A, Kobilka, TS, Yao, X-J, Choi, H-J, Weis, WI, Rohrer, DK & Kobilka, BK 2007, 'A monoclonal antibody for G protein-coupled receptor crystallography', Nature Methods, vol. 4, no. 11, pp. 927-9. https://doi.org/10.1038/nmeth1112

APA

Day, P. W., Rasmussen, S. G. F., Parnot, C., Fung, J. J., Masood, A., Kobilka, T. S., Yao, X-J., Choi, H-J., Weis, W. I., Rohrer, D. K., & Kobilka, B. K. (2007). A monoclonal antibody for G protein-coupled receptor crystallography. Nature Methods, 4(11), 927-9. https://doi.org/10.1038/nmeth1112

Vancouver

Day PW, Rasmussen SGF, Parnot C, Fung JJ, Masood A, Kobilka TS et al. A monoclonal antibody for G protein-coupled receptor crystallography. Nature Methods. 2007 Nov;4(11):927-9. https://doi.org/10.1038/nmeth1112

Author

Day, Peter W ; Rasmussen, Søren Gøgsig Faarup ; Parnot, Charles ; Fung, Juan José ; Masood, Asna ; Kobilka, Tong Sun ; Yao, Xiao-Jie ; Choi, Hee-Jung ; Weis, William I ; Rohrer, Daniel K ; Kobilka, Brian K. / A monoclonal antibody for G protein-coupled receptor crystallography. In: Nature Methods. 2007 ; Vol. 4, No. 11. pp. 927-9.

Bibtex

@article{3046f26475f4463ab0b81a2d394cefcc,
title = "A monoclonal antibody for G protein-coupled receptor crystallography",
abstract = "G protein-coupled receptors (GPCRs) constitute the largest family of signaling proteins in mammals, mediating responses to hormones, neurotransmitters, and senses of sight, smell and taste. Mechanistic insight into GPCR signal transduction is limited by a paucity of high-resolution structural information. We describe the generation of a monoclonal antibody that recognizes the third intracellular loop (IL3) of the native human beta(2) adrenergic (beta(2)AR) receptor; this antibody was critical for acquiring diffraction-quality crystals.",
keywords = "Amino Acid Sequence, Animals, Antibodies, Monoclonal, Antigen-Antibody Reactions, Blotting, Western, Crystallization, Crystallography, Epitopes, Fluorescent Dyes, Humans, Immunoglobulin Fab Fragments, Mice, Molecular Sequence Data, Protein Structure, Tertiary, Receptors, Adrenergic, beta-2, Receptors, G-Protein-Coupled, Recombinant Proteins, Rhodamines, Vaccination",
author = "Day, {Peter W} and Rasmussen, {S{\o}ren G{\o}gsig Faarup} and Charles Parnot and Fung, {Juan Jos{\'e}} and Asna Masood and Kobilka, {Tong Sun} and Xiao-Jie Yao and Hee-Jung Choi and Weis, {William I} and Rohrer, {Daniel K} and Kobilka, {Brian K}",
year = "2007",
month = nov,
doi = "10.1038/nmeth1112",
language = "English",
volume = "4",
pages = "927--9",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "nature publishing group",
number = "11",

}

RIS

TY - JOUR

T1 - A monoclonal antibody for G protein-coupled receptor crystallography

AU - Day, Peter W

AU - Rasmussen, Søren Gøgsig Faarup

AU - Parnot, Charles

AU - Fung, Juan José

AU - Masood, Asna

AU - Kobilka, Tong Sun

AU - Yao, Xiao-Jie

AU - Choi, Hee-Jung

AU - Weis, William I

AU - Rohrer, Daniel K

AU - Kobilka, Brian K

PY - 2007/11

Y1 - 2007/11

N2 - G protein-coupled receptors (GPCRs) constitute the largest family of signaling proteins in mammals, mediating responses to hormones, neurotransmitters, and senses of sight, smell and taste. Mechanistic insight into GPCR signal transduction is limited by a paucity of high-resolution structural information. We describe the generation of a monoclonal antibody that recognizes the third intracellular loop (IL3) of the native human beta(2) adrenergic (beta(2)AR) receptor; this antibody was critical for acquiring diffraction-quality crystals.

AB - G protein-coupled receptors (GPCRs) constitute the largest family of signaling proteins in mammals, mediating responses to hormones, neurotransmitters, and senses of sight, smell and taste. Mechanistic insight into GPCR signal transduction is limited by a paucity of high-resolution structural information. We describe the generation of a monoclonal antibody that recognizes the third intracellular loop (IL3) of the native human beta(2) adrenergic (beta(2)AR) receptor; this antibody was critical for acquiring diffraction-quality crystals.

KW - Amino Acid Sequence

KW - Animals

KW - Antibodies, Monoclonal

KW - Antigen-Antibody Reactions

KW - Blotting, Western

KW - Crystallization

KW - Crystallography

KW - Epitopes

KW - Fluorescent Dyes

KW - Humans

KW - Immunoglobulin Fab Fragments

KW - Mice

KW - Molecular Sequence Data

KW - Protein Structure, Tertiary

KW - Receptors, Adrenergic, beta-2

KW - Receptors, G-Protein-Coupled

KW - Recombinant Proteins

KW - Rhodamines

KW - Vaccination

U2 - 10.1038/nmeth1112

DO - 10.1038/nmeth1112

M3 - Journal article

C2 - 17952087

VL - 4

SP - 927

EP - 929

JO - Nature Methods

JF - Nature Methods

SN - 1548-7091

IS - 11

ER -

ID: 120588791