A metallothionein mimetic peptide protects neurons against kainic acid-induced excitotoxicity
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A metallothionein mimetic peptide protects neurons against kainic acid-induced excitotoxicity. / Sonn, Katrin; Pankratova, Stanislava; Korshunova, Irina; Zharkovsky, Alexander; Bock, Elisabeth; Berezin, Vladimir; Kiryushko, Darya.
In: Journal of Neuroscience Research, Vol. 88, No. 5, 2010, p. 1074-82.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - A metallothionein mimetic peptide protects neurons against kainic acid-induced excitotoxicity
AU - Sonn, Katrin
AU - Pankratova, Stanislava
AU - Korshunova, Irina
AU - Zharkovsky, Alexander
AU - Bock, Elisabeth
AU - Berezin, Vladimir
AU - Kiryushko, Darya
N1 - Keywords: Animals; Blood-Brain Barrier; Cells, Cultured; Cerebral Cortex; Disease Models, Animal; Dose-Response Relationship, Drug; Epilepsy, Temporal Lobe; Hippocampus; Kainic Acid; Male; Metallothionein; Mice; Mice, Inbred C57BL; Nerve Degeneration; Neuroprotective Agents; Neurotoxins; Peptides; Rats; Rats, Wistar; Seizures
PY - 2010
Y1 - 2010
N2 - Metallothioneins I and II (MTI/II) are metal-binding proteins overexpressed in response to brain injury. Recently, we have designed a peptide, termed EmtinB, which is modeled after the beta-domain of MT-II and mimics the biological effects of MTI/II in vitro. Here, we demonstrate the neuroprotective effect of EmtinB in the in vitro and in vivo models of kainic acid (KA)-induced neurotoxicity. We show that EmtinB passes the blood-brain barrier and is detectable in plasma for up to 24 hr. Treatment with EmtinB significantly attenuates seizures in C57BL/6J mice exposed to moderate (20 mg/kg) and high (30 mg/kg) KA doses and tends to decrease mortality induced by the high KA dose. Histopathological evaluation of hippocampal (CA3 and CA1) and cortical areas of mice treated with 20 mg/kg KA shows that EmtinB treatment reduces KA-induced neurodegeneration in the CA1 region. These findings establish EmtinB as a promising target for therapeutic development.
AB - Metallothioneins I and II (MTI/II) are metal-binding proteins overexpressed in response to brain injury. Recently, we have designed a peptide, termed EmtinB, which is modeled after the beta-domain of MT-II and mimics the biological effects of MTI/II in vitro. Here, we demonstrate the neuroprotective effect of EmtinB in the in vitro and in vivo models of kainic acid (KA)-induced neurotoxicity. We show that EmtinB passes the blood-brain barrier and is detectable in plasma for up to 24 hr. Treatment with EmtinB significantly attenuates seizures in C57BL/6J mice exposed to moderate (20 mg/kg) and high (30 mg/kg) KA doses and tends to decrease mortality induced by the high KA dose. Histopathological evaluation of hippocampal (CA3 and CA1) and cortical areas of mice treated with 20 mg/kg KA shows that EmtinB treatment reduces KA-induced neurodegeneration in the CA1 region. These findings establish EmtinB as a promising target for therapeutic development.
U2 - 10.1002/jnr.22281
DO - 10.1002/jnr.22281
M3 - Journal article
C2 - 19937811
VL - 88
SP - 1074
EP - 1082
JO - Journal of Neuroscience Research
JF - Journal of Neuroscience Research
SN - 0360-4012
IS - 5
ER -
ID: 21602373