A Latent Propriospinal Network Can Restore Diaphragm Function after High Cervical Spinal Cord Injury

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

A Latent Propriospinal Network Can Restore Diaphragm Function after High Cervical Spinal Cord Injury. / Cregg, Jared M; Chu, Kevin A; Hager, Lydia E; Maggard, Rachel S J; Stoltz, Daimen R; Edmond, Michaela; Alilain, Warren J; Philippidou, Polyxeni; Landmesser, Lynn T; Silver, Jerry.

In: Cell Reports, Vol. 21, No. 3, 17.10.2017, p. 654-665.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Cregg, JM, Chu, KA, Hager, LE, Maggard, RSJ, Stoltz, DR, Edmond, M, Alilain, WJ, Philippidou, P, Landmesser, LT & Silver, J 2017, 'A Latent Propriospinal Network Can Restore Diaphragm Function after High Cervical Spinal Cord Injury', Cell Reports, vol. 21, no. 3, pp. 654-665. https://doi.org/10.1016/j.celrep.2017.09.076

APA

Cregg, J. M., Chu, K. A., Hager, L. E., Maggard, R. S. J., Stoltz, D. R., Edmond, M., Alilain, W. J., Philippidou, P., Landmesser, L. T., & Silver, J. (2017). A Latent Propriospinal Network Can Restore Diaphragm Function after High Cervical Spinal Cord Injury. Cell Reports, 21(3), 654-665. https://doi.org/10.1016/j.celrep.2017.09.076

Vancouver

Cregg JM, Chu KA, Hager LE, Maggard RSJ, Stoltz DR, Edmond M et al. A Latent Propriospinal Network Can Restore Diaphragm Function after High Cervical Spinal Cord Injury. Cell Reports. 2017 Oct 17;21(3):654-665. https://doi.org/10.1016/j.celrep.2017.09.076

Author

Cregg, Jared M ; Chu, Kevin A ; Hager, Lydia E ; Maggard, Rachel S J ; Stoltz, Daimen R ; Edmond, Michaela ; Alilain, Warren J ; Philippidou, Polyxeni ; Landmesser, Lynn T ; Silver, Jerry. / A Latent Propriospinal Network Can Restore Diaphragm Function after High Cervical Spinal Cord Injury. In: Cell Reports. 2017 ; Vol. 21, No. 3. pp. 654-665.

Bibtex

@article{9a546875357d4916ae178fbc6046c3a5,
title = "A Latent Propriospinal Network Can Restore Diaphragm Function after High Cervical Spinal Cord Injury",
abstract = "Spinal cord injury (SCI) above cervical level 4 disrupts descending axons from the medulla that innervate phrenic motor neurons, causing permanent paralysis of the diaphragm. Using an ex vivo preparation in neonatal mice, we have identified an excitatory spinal network that can direct phrenic motor bursting in the absence of medullary input. After complete cervical SCI, blockade of fast inhibitory synaptic transmission caused spontaneous, bilaterally coordinated phrenic bursting. Here, spinal cord glutamatergic neurons were both sufficient and necessary for the induction of phrenic bursts. Direct stimulation of phrenic motor neurons was insufficient to evoke burst activity. Transection and pharmacological manipulations showed that this spinal network acts independently of medullary circuits that normally generate inspiration, suggesting a distinct non-respiratory function. We further show that this {"}latent{"} network can be harnessed to restore diaphragm function after high cervical SCI in adult mice and rats.",
keywords = "Animals, Animals, Newborn, Cervical Vertebrae/physiopathology, Diaphragm/innervation, Interneurons/pathology, Light, Lumbar Vertebrae/physiopathology, Mice, Motor Neurons/pathology, Nerve Net/physiopathology, Paralysis/physiopathology, Phrenic Nerve/physiopathology, Respiration, Spinal Cord Injuries/physiopathology, Synaptic Transmission/physiology, Thoracic Vertebrae/physiopathology",
author = "Cregg, {Jared M} and Chu, {Kevin A} and Hager, {Lydia E} and Maggard, {Rachel S J} and Stoltz, {Daimen R} and Michaela Edmond and Alilain, {Warren J} and Polyxeni Philippidou and Landmesser, {Lynn T} and Jerry Silver",
note = "Copyright {\textcopyright} 2017 The Author(s). Published by Elsevier Inc. All rights reserved.",
year = "2017",
month = oct,
day = "17",
doi = "10.1016/j.celrep.2017.09.076",
language = "English",
volume = "21",
pages = "654--665",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "3",

}

RIS

TY - JOUR

T1 - A Latent Propriospinal Network Can Restore Diaphragm Function after High Cervical Spinal Cord Injury

AU - Cregg, Jared M

AU - Chu, Kevin A

AU - Hager, Lydia E

AU - Maggard, Rachel S J

AU - Stoltz, Daimen R

AU - Edmond, Michaela

AU - Alilain, Warren J

AU - Philippidou, Polyxeni

AU - Landmesser, Lynn T

AU - Silver, Jerry

N1 - Copyright © 2017 The Author(s). Published by Elsevier Inc. All rights reserved.

PY - 2017/10/17

Y1 - 2017/10/17

N2 - Spinal cord injury (SCI) above cervical level 4 disrupts descending axons from the medulla that innervate phrenic motor neurons, causing permanent paralysis of the diaphragm. Using an ex vivo preparation in neonatal mice, we have identified an excitatory spinal network that can direct phrenic motor bursting in the absence of medullary input. After complete cervical SCI, blockade of fast inhibitory synaptic transmission caused spontaneous, bilaterally coordinated phrenic bursting. Here, spinal cord glutamatergic neurons were both sufficient and necessary for the induction of phrenic bursts. Direct stimulation of phrenic motor neurons was insufficient to evoke burst activity. Transection and pharmacological manipulations showed that this spinal network acts independently of medullary circuits that normally generate inspiration, suggesting a distinct non-respiratory function. We further show that this "latent" network can be harnessed to restore diaphragm function after high cervical SCI in adult mice and rats.

AB - Spinal cord injury (SCI) above cervical level 4 disrupts descending axons from the medulla that innervate phrenic motor neurons, causing permanent paralysis of the diaphragm. Using an ex vivo preparation in neonatal mice, we have identified an excitatory spinal network that can direct phrenic motor bursting in the absence of medullary input. After complete cervical SCI, blockade of fast inhibitory synaptic transmission caused spontaneous, bilaterally coordinated phrenic bursting. Here, spinal cord glutamatergic neurons were both sufficient and necessary for the induction of phrenic bursts. Direct stimulation of phrenic motor neurons was insufficient to evoke burst activity. Transection and pharmacological manipulations showed that this spinal network acts independently of medullary circuits that normally generate inspiration, suggesting a distinct non-respiratory function. We further show that this "latent" network can be harnessed to restore diaphragm function after high cervical SCI in adult mice and rats.

KW - Animals

KW - Animals, Newborn

KW - Cervical Vertebrae/physiopathology

KW - Diaphragm/innervation

KW - Interneurons/pathology

KW - Light

KW - Lumbar Vertebrae/physiopathology

KW - Mice

KW - Motor Neurons/pathology

KW - Nerve Net/physiopathology

KW - Paralysis/physiopathology

KW - Phrenic Nerve/physiopathology

KW - Respiration

KW - Spinal Cord Injuries/physiopathology

KW - Synaptic Transmission/physiology

KW - Thoracic Vertebrae/physiopathology

U2 - 10.1016/j.celrep.2017.09.076

DO - 10.1016/j.celrep.2017.09.076

M3 - Journal article

C2 - 29045834

VL - 21

SP - 654

EP - 665

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 3

ER -

ID: 248114012