TY - JOUR

T1 - Regulation of activity-regulated cytoskeleton protein (Arc) mRNA after acute and chronic electroconvulsive stimulation in the rat.

AU - Larsen, M H

AU - Olesen, M

AU - Woldbye, D P D

AU - Hay-Schmidt, A

AU - Hansen, H H

AU - Rønn, L C B

AU - Mikkelsen, J D

N1 - Keywords: Animals; Cerebral Cortex; Cytoskeletal Proteins; Depressive Disorder; Disease Models, Animal; Electroconvulsive Therapy; Electroshock; Gene Expression Regulation; Hippocampus; Male; Memory; Nerve Tissue Proteins; Parietal Lobe; RNA, Messenger; Rats; Rats, Sprague-Dawley

PY - 2005

Y1 - 2005

N2 - The temporal profile of Arc gene expression after acute and chronic electroconvulsive stimulations (ECS) was studied using semi-quantitative in situ hybridisation in the rat cortex. A single ECS strongly and temporarily increased Arc mRNA levels in dentate granular cells with maximal induction seen up to 4 h after the stimulus, but returned to baseline at 24 h. A single ECS also increased expression of Arc mRNA in the CA1 and the parietal cortex, but the expression peaked within 1 h and returned to baseline levels within 2 h. Repeated or chronic ECS is a model of electroconvulsive therapy and it would be predicted that gene products involved in antidepressant effects accumulate after repeated ECS. However, repeated ECS reduced Arc gene expression in the CA1 24 h after the last stimulus. These results indicate that Arc is an immediate early gene product regulated by an acute excitatory stimulus, but not accumulated by long term repetitive ECS and therefore not a molecular biomarker for antidepressant properties. More likely, Arc is likely a molecular link to the decline in memory consolidation seen in depressive patients subjected to electroconvulsive therapy.

AB - The temporal profile of Arc gene expression after acute and chronic electroconvulsive stimulations (ECS) was studied using semi-quantitative in situ hybridisation in the rat cortex. A single ECS strongly and temporarily increased Arc mRNA levels in dentate granular cells with maximal induction seen up to 4 h after the stimulus, but returned to baseline at 24 h. A single ECS also increased expression of Arc mRNA in the CA1 and the parietal cortex, but the expression peaked within 1 h and returned to baseline levels within 2 h. Repeated or chronic ECS is a model of electroconvulsive therapy and it would be predicted that gene products involved in antidepressant effects accumulate after repeated ECS. However, repeated ECS reduced Arc gene expression in the CA1 24 h after the last stimulus. These results indicate that Arc is an immediate early gene product regulated by an acute excitatory stimulus, but not accumulated by long term repetitive ECS and therefore not a molecular biomarker for antidepressant properties. More likely, Arc is likely a molecular link to the decline in memory consolidation seen in depressive patients subjected to electroconvulsive therapy.

U2 - 10.1016/j.brainres.2005.09.039

DO - 10.1016/j.brainres.2005.09.039

M3 - Journal article

C2 - 16309632

VL - 1064

SP - 161

EP - 165

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1-2

ER -