A possible postsynaptic role for SNAP-25 in hippocampal synapses
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A possible postsynaptic role for SNAP-25 in hippocampal synapses. / Hussain, S.; Ringsevjen, H.; Schupps, M.; Hvalby, Ø.; Sørensen, J. B.; Jensen, V.; Davanger, S.
In: Brain Structure and Function, Vol. 224, No. 2, 2019, p. 521-532.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - A possible postsynaptic role for SNAP-25 in hippocampal synapses
AU - Hussain, S.
AU - Ringsevjen, H.
AU - Schupps, M.
AU - Hvalby, Ø.
AU - Sørensen, J. B.
AU - Jensen, V.
AU - Davanger, S.
N1 - Correction to: A possible postsynaptic role for SNAP-25 in hippocampal synapses DOI: 10.1007/s00429-018-01823-3
PY - 2019
Y1 - 2019
N2 - The SNARE protein SNAP-25 is well documented as regulator of presynaptic vesicle exocytosis. Increasing evidence suggests roles for SNARE proteins in postsynaptic trafficking of glutamate receptors as a basic mechanism in synaptic plasticity. Despite these indications, detailed quantitative subsynaptic localization studies of SNAP-25 have never been performed. Here, we provide novel electron microscopic data of SNAP-25 localization in postsynaptic spines. In addition to its expected presynaptic localization, we show that the protein is also present in the postsynaptic density (PSD), the postsynaptic lateral membrane and on small vesicles in the postsynaptic cytoplasm. We further investigated possible changes in synaptic SNAP-25 protein expression after hippocampal long-term potentiation (LTP). Quantitative analysis of immunogold-labeled electron microscopy sections did not show statistically significant changes of SNAP-25 gold particle densities 1 h after LTP induction, indicating that local trafficking of SNAP-25 does not play a role in the early phases of LTP. However, the strong expression of SNAP-25 in postsynaptic plasma membranes suggests a function of the protein in postsynaptic vesicle exocytosis and a possible role in hippocampal synaptic plasticity.
AB - The SNARE protein SNAP-25 is well documented as regulator of presynaptic vesicle exocytosis. Increasing evidence suggests roles for SNARE proteins in postsynaptic trafficking of glutamate receptors as a basic mechanism in synaptic plasticity. Despite these indications, detailed quantitative subsynaptic localization studies of SNAP-25 have never been performed. Here, we provide novel electron microscopic data of SNAP-25 localization in postsynaptic spines. In addition to its expected presynaptic localization, we show that the protein is also present in the postsynaptic density (PSD), the postsynaptic lateral membrane and on small vesicles in the postsynaptic cytoplasm. We further investigated possible changes in synaptic SNAP-25 protein expression after hippocampal long-term potentiation (LTP). Quantitative analysis of immunogold-labeled electron microscopy sections did not show statistically significant changes of SNAP-25 gold particle densities 1 h after LTP induction, indicating that local trafficking of SNAP-25 does not play a role in the early phases of LTP. However, the strong expression of SNAP-25 in postsynaptic plasma membranes suggests a function of the protein in postsynaptic vesicle exocytosis and a possible role in hippocampal synaptic plasticity.
KW - Electron microscopy
KW - Hippocampus
KW - LTP
KW - SNARE proteins
KW - Synaptic plasticity
UR - https://doi.org/10.1007/s00429-018-01823-3
U2 - 10.1007/s00429-018-1782-2
DO - 10.1007/s00429-018-1782-2
M3 - Journal article
C2 - 30377802
AN - SCOPUS:85055738116
VL - 224
SP - 521
EP - 532
JO - Brain Structure and Function
JF - Brain Structure and Function
SN - 1863-2653
IS - 2
ER -
ID: 209804655