Techniques

  • Protein expression (eg. baculovirus-mediated expression in insect and mammalian cell cultures, bacterial expression)
  • Biochemistry (purification, reconstitution, Western blotting, analytical SEC, Co-IP, site-directed fluorescent labeling, site-directed cleavage, cross-linking, preparation of Fab fragments, scFv and nanobodies etc.)
  • Molecular pharmacology (radioligand saturation and competition binding, various radioactivity-based G protein coupling assays, uptake assays for radiolabeled substrates)
  • Molecular biology (plasmid preparation, site-directed mutagenesis, subcloning etc.)
  • Immunocytochemistry
  • Fluorescence spectroscopy
  • Protein crystallography and cryoEM