Publication List – University of Copenhagen

Publication List


Peer review

37    McMahon C., Baier A.S., Pascolutti R., Wegrecki M., Zheng S., Ong J.X., Erlandson S.C., Hilger D., Rasmussen S.G.F., Ring A.M., Manglik A., Kruse A.C., Yeast surface display platform for rapid discovery of conformationally selective nanobodies, Nature Structural and Molecular Biology (2018), in press

36    Boyhus L.E., Danielsen M., Bengtson N.S., Kunze M.B.A., Kubiak X., Sminia T.J., Løper J.H., Tran P.T., Lindorff-Larsen K., Rasmussen S.G.F., Mathiesen J.M., Pedersen D.S., Gs Protein peptidomimetics as allosteric modulators of the β2-adrenergic receptor, RSC Advances (2018) 8:2219-2238

35    Clark L., Dikiy I., Chapman K., Rödström K.E.J., Aramini J., LeVine M.V., Khelashvili G., Rasmussen S.G.F., Gardner K.H., Rosenbaum D.M., Ligand modulation of sidechain dynamics in a wild-type human GPCR, Elife (2017), DOI:

34    DeVree B.T., Mahoney J.P., Vélez-Ruiz G.A., Rasmussen S.G.F, Kuszak A.J., Edwald E., Fung J.J., Manglik A., Masureel M., Du Y., Matt R.A., Pardon E., Steyaert J., Kobilka B.K. and Sunahara R.K., Allosteric coupling from G protein to the agonist binding pocket in GPCRs. Nature (2016) 535:182-6

33    Mathiesen S., Christensen S.M., Fung J.J., Rasmussen S.G.F.,  Fay J.F., Jørgensen S.K., Farrens D.L., Byrne M., Kobilka B., Stamou D., High-content analysis of single proteoliposome composition used to assay quantitatively GPCR oligomerization Nature Methods (2014) 11:931-4

32    Pardon E., Laeremans T., Triest S., Rasmussen S.G.F., Wohlkonig A., Ruf A., Muyldermans S., Hol W., Kobilks B.K., Steyaert J. A General Protocol for Generation of Nanobodies for Structural Biology. Nature Protocols (2014) 9:674-693

31    Staus D., Wingler L.M., Strachan R.T., Rasmussen S.G.F., Pardon E., Ahn S., Steyaert J., Kobilka B., Lefkowitz R.J., Regulation of β2‐Adrenergic Receptor‐2 Function by Conformationally Selective Single‐domain Intrabodies. Mol. Pharmacol.  (2014) 85:472-481

30    Chae P.S., Kruse A.C., Gotfryd K., Rana R.R., Rasmussen S.G.F., Cho K.H., Bae E., Guan L., Loland C.J., Kobilka B., Byrne B., Gellman S.H. Novel tripod amphiphiles for membrane protein analysis. Chem. Eur. J. (2013) 19:15645-15651

29    Irannejad R., Tomshine J.C., Tomshine J.R., Chevalier M., Mahoney J.P., Steyaert J., Rasmussen S.G.F., Sunahara R.K., El-Samad H., Huang B., von Zastrow M. Conformational biosensors reveal GPCR signaling from endosomes, Nature (2013) 495:534-538

28    Chae P.S., Rana R.R., Gotfryd K., Rasmussen S.G.F.,  Kruse A.C., Cho K.H., Capaldi S., Kobilka B., Loland C.J., Gether U., Banerjee S., Byrne B., Lee J., Gellman S.H. Glucose-Neopentyl Glycol (GNG) amphiphiles for Membrane Protein Solubilization, Stabilization and Crystallization. ChemCommun (2013) 49:2287-89

27    Zocher M., Zhang C., Rasmussen S.G.F., Kobilka B.K., Müller D.J., Cholesterol increases the kinetic, energetic, and mechanical stability of the human β2 adrenergic receptor. Proc. Natl. Acad. Sci. USA. (2012) 109:3463-72

26    Chae P.S.., Rasmussen S.G.F.,  Rana R.R., Gotfryd K., Kruse A.C., Nurva S., Gether U., Guan L., Loland C.J., Byrne B., Kobilka B., Gellman S.H. A New Class of Amphiphiles Bearing Rigid Hydrophobic Groups for Solubilization and Stabilization of Membrane Proteins. Chem. Eur. J. (2012)

25    Chung K.Y§, Rasmussen S.G.F.§, Liu T. §, Li S., DeVree B.T., Chae P.S., Calinski D., Kobilka B.K., Woods V.L., Sunahara R.K. b2 Adrenergic Receptor-Induced conformational changes in the heterotrimeric G protein. Nature (2011) 477: 611-615. §Authors contributed equally to this work

24    Westfield G.§, Rasmussen S.G.F.§, Su M. §, Dutta S. §, DeVree B.T., Chung K.Y., Calinski D., Velez-Ruiz G., Oleskie A., Pardon E., Chae P.S., Liu T., Li. S., Woods V.L., Steyeart J., Kobilka B.K., Sunahara R.K., Skiniotis G., Structural Rearrangements of the Gas a-Helical Domain in the b2-adrenoceptor Gs Complex. Proc. Natl. Acad. Sci. USA. (2011) 108:16086-91. § Authors contributed equally to this work

23    Rasmussen S.G.F. §, DeVree B.T. §, Zho Y., Kruse A.C., Chung K.Y., Kobilka T.S., Thian F.S., Chae P.S., Pardon E., Calinski D., Mathiesen J.M., Shah S.T.A., Lyons J.A., Caffrey M., Gellman S.H., Steyaert J., Skiniotis G., Weis W.I., Sunahara R.K., Kobilka B.K., Crystal Structure of the b2 Adrenergic Receptor-Gs Protein Complex. Nature (2011) 477:550-555.  §Authors contributed equally to this work

22    Selao T.T., Branca R. , Chae P.S., Lehtiö J., Gellman S.H., Rasmussen S.G.F., Nordlund S., Norén A., Identification of the chromophore membrane protein complex formed under different nitrogen availability conditions in Rhodospirilium rubrum. J. Prot. Res. (2011) 10:2703-2714

21    Rosenbaum D.M., Zhang C., Lyons J., Holl R., Aragao D., Arlow D.H., Rasmussen S.G.F., Choi H.J., DeVree B.T., Sunahara R.K., Chae P.S., Gellman S.H., Dror R.O., Shaw D.E., Weis W.I., Caffrey M., Gmeiner P., Kobilka B.K., Structure and Function of an Irreversible Agonist-b2 Adrenoceptor Complex,  Nature  (2011) 469: 236-240

20    Rasmussen S.G.F. §, Choi H.J. §, Fung J.J.§, Casarosa P., Pardon E., Chae P.S., DeVree B.T., Rosenbaum D.M., Kobilka T.S., Thian F.S., Schnapp A., Konetzki I., Sunahara R.K., Gellman S.H., Pautsch A., Steyaert J., Weis W.I., Kobilka B.K., Structure of an Nanobody-Stabilized Active State of the b2 Adrenoceptor, Nature (2011) 469:175-180. §Authors contributed equally to this work

19    Chae P.S., Gotfryd K., Pecyna J., Miercke L.J.W., Rasmussen S.G.F., Robins R.A., Loland C.J., Kobilka B., Stroud R., Byrne B., Gether U., Gellman S.H. Tandem Facial Amphiphiles for Membrane Protein Stabilization. J. Am. Chem. Soc. (2010) 132:16750-16752

18    Chae P.S., Rasmussen S.G.F., Rana R., Gotfryd K., Chandra R., Goren M.A., Kruse A.C., Nurva S., Loland C.J., Pierre Y., Drew D., Popot J.L. Picot D., Fox B.G., Guan L., Gether U., Byrne B., Kobilka B., Gellman S.H., Maltose-neopentyl glycol (MNG) amphiphiles for solubilization, stabilization and crystallization of membrane proteins. Nature Methods  (2010) 7:1003-8

17    Bokoch M.P., Zou Y., Rasmussen S.G.F., Liu C.W., Nygaard R.,  Rosenbaum D.M., Fung J.J., Choi H.J., Thian F.S., Kobilka T.S., Puglisi J.D., Weis W.I., Pardo L., Prossor R.S., Mueller L., and Kobilka B.K., Ligand-specific regulation of the extracellular surface of a G protein coupled receptor. Nature (2010) 463:108-112

16    Eriksen J.§, Rasmussen S.G.F. §,  Rasmussen T.N., Vaegter C.B., Cha J.H., Zhu M.F., Fog J.U., J.H., Newman A.H., Gether U., Visualization of dopamine transporter trafficking in live neurons by use of fluorescent cocaine analogues. J. Neuroscience (2009) 29:6794-808. § Authors contributed equally to this work

15     Yao Y.J., Whorton M.R., Vélez-Ruiz G., Rasmussen S.G.F., DeVree B.T., Deupi X., Sunahara R.K., and Kobilka B.K., The effect of ligand efficacy on the formation and stability of a GPCR-G protein complex.  Proc. Natl. Acad. Sci. USA. (2009) 106:9501-9506 

14    Richter W., Day P.W., Agrawal R., Bruss M., Granier S., Wang Y., Rasmussen S.G.F., Horner K., Wang P., Lei T., Patterson A.J., Kobilka B., and Conti M., Signaling from b1- and b2-adrenergic receptors is defined by differential interactions with PDE4. EMBO J. (2008) 27:384-393

13    Cherezov V., Rosenbaum D.M., Hanson M.A., Rasmussen S.G.F., Thian F.S, Kobilka T.S, Choi H.J., Kuhn P., Weis W.I., Kobilka B.K, Stevens R.C, High Resolution Crystal Structure of a Modified Human b2-Adrenergic G protein-Coupled Receptor, Science (2007) 318:1258-1265  

12    Rosenbaum D.M., Cherezov V., Hanson M.A., Rasmussen S.G.F., Thian F.S., Kobilka T.S., Choi H.J., Yao Y.J., Kuhn P., Weis W.I., Stevens R.C., Kobilka B.K, GPCR Engineering Yields High-resolution Structural Insights into b2 Adrenergic Receptor Function, Science (2007) 318:1266-1273

11    Rasmussen S.G.F. §, Choi H.J. §, Rosenbaum D.M. §, Kobilka T.S., Thian F.S., Edwards P., Burghammer M., Ratnala V.R.P., Sanishvili R., Fischetti R., Schertler G.F.X, Weis W.I., Kobilka B.K., Crystal structure of the human b2 adrenergic G-protein-coupled receptor, Nature (2007) 450:383-387. § Authors contributed equally to this work

10    Day P.W.§, Rasmussen S.G.F.§,  Parnot C., Fung J.J., Masood A., Kobilka T.S., Yao Y.J., Choi H.J., Weis W.I., Rohrer D.K., Kobilka B.K., A monoclonal antibody for G protein coupled receptor crystallography, Nature Methods (2007) 4:927-929. § Authors contributed equally to this work.

9      Plenge P., Gether U., and Rasmussen S.G.F. Allosteric effects of R- and S-citalopram on the human 5-HT transporter: Evidence for distinct high- and low-affinity binding sites. Eur.  J. Pharmacol. (2007) 567:1-9

8      Whorton M.R., Bokoch M.P., Rasmussen S.G.F., Huang B., Zare R.N., Kobilka B., and Sunahara R.K. A monomeric G Protein coupled receptor isolated in a high-density lipoprotein particle efficiently activates its G protein. Proc. Natl. Acad. Sci. USA. (2007) 104:7682-7687

7      Cha J.H., Zhu M.F., Adkins E.M., Rasmussen S.G.F., Loland C.J., Schonenberger B., Gether U., Newman A.H., Rhodamine-Labeled 2b-Carbomethoxy-3b-(3,4-dichlorophenyl)tropane Analogues as High-Affinity Fluorescent Probes for the Dopamine Transporter, J. Med. Chem. (2005) 48:7513-7516

6      Rasmussen S.G.F. and Gether U., Purification and Fluorescent Labeling of the Human Serotonin Transporter, Biochemistry (2005) 44: 3494-3505

5      Ballesteros J.A. §, Jensen A.D.§, Liapakis G. §, Rasmussen S.G.F.§,  Shi L., Gether U., Javitch J.A., Activation of the b2 Adrenergic Receptor Involves Disruption of an Ionic Lock Between the Cytoplasmic Ends of Transmembrane Segments 3 and 6, J. Biol. Chem. (2001) 276:29171-29177. § Authors contributed equally to this work.

4      Jensen A.D., Guarnieri F., Rasmussen S.G.F., Asmar F., Ballesteros, J.A., Gether U, Agonist-Induced Conformational Changes at the Cytoplasmic Side of TM6 in the b2 Adrenergic Receptor Mapped by Site-Selective Fluorescent Labeling, J. Biol. Chem. (2001) 276: 9279-9290.

3      Rasmussen S.G.F., Carroll F.I., Maresch M.,  Jensen A.D., Tate C.G., Gether U., Biophysical Characterization of the Cocaine Binding Pocket in the Serotonin Transporter Using a Fluorescent Cocaine Analogue as a Molecular Reporter, J. Biol. Chem. (2001) 276: 4717-4723.

2      Ghanouni P., Schambye H., Seifert R., Lee T.W., Rasmussen S.G.F., Gether U., Kobilka B. K., The Effect of pH on b2 Adrenoceptor Function: Evidence for protonation-dependent activation, J. Biol. Chem. (2000) 275: 3121-3127.

1        Rasmussen S.G.F., Jensen A.D., Liapakis G., Ghanouni P., Javitch J.A.,  Gether U., Mutation of a Highly Conserved Aspartic Acid in the b2 Adrenergic Receptor: Constitutive Activation, Structural Instability and Conformational Rearrangement of Transmembrane Segment 6, Mol. Pharmacol. (1999), 56: 175-184


3      Gether U., Asmar F., Meinild A.K., Rasmussen S.G.F. Structural basis for G-protein coupled receptor activation, Pharmacology and Toxicology (2002) 91: 304-312.  

2      Rasmussen S.G.F., Adkins E.M., Carroll F.I., Maresch M., Gether U. Structural and Functional Probing of the Biogenic Amine Transporters by Fluorescence Spectroscopy, Eur.  J.  Pharmacol., (2003) 479: 13-22.

1      Rosenbaum D.M. Rasmussen S.G.F., and Kobilka B.K, Structural and mechanistic insights into G-protein coupled receptor function, Nature (2009) 459:356-363



5      Rasmussen S.G.F. and Gether U., Structural mechanics of GPCR activation, In Understanding G-protein coupled receptors and their role in the CNS (eds. M.N. Pangalos and C.H. Davies), ISBN: 0-19-850916-2, Oxford University Press, Oxford: New York, pp. 43-62 (2002)

4      Rasmussen S.G.F. and Gether U., Molecular mechanisms of GPCR activation, In G Protein-Coupled Receptors as Drug Targets: Analysis of Activation and Constitutive Activity, Volume 24 (eds. R. Seifert and T. Wieland), ISBN: 3-527-30819-9, WILEY-VCH Verlag GmbH, Weinheim, pp. 27-42 (2006)

3      Weis W.I., Rosenbaum D.M., Rasmussen S.G.F., et. al. Crystal Structures of the β2-Adrenergic Receptor, In From Molecules to Medicines, Structure of Biological Macromolecules and Its Relevance in Combating New Diseases and Bioterrorism (eds. J.L. Sussman and P. Spadon) ISBN:  978-90-481-2338-4, Springer Netherlands, pp. 217-230 (2009)

2      Rosenbaum D.M. Rasmussen S.G.F., and Kobilka B.K, The b2 Adrenergic Receptor  as a Model for G Protein-Coupled Receptor Structure and Activation by Diffusible Hormones, In The Handbook of Cell Signaling (eds. R. Bradshaw and E. Dennis), ISBN-10: 0-12-374145-9, Elsevier Inc., (2009)

1      Kuszak A.J., Yao X.J., Rasmussen S.G.F, Kobilka B.K., and Sunahara R.K., Functional studies of isolated GPCR-G protein complexes in the membrane bilayer of lipoprotein particles, In G Protein-Coupled Receptors (eds. S. Siehler and and G. Milligan), ISBN-978-0-521-11208-6, Cambridge University Press, pp.32-52 (2010)