Department of Neuroscience > Research Labs > Madsen Lab > Techniques
Techniques
- Protein trafficking of transmembrane proteins (by flowcytometry, confocal microscopy, TIRF microscopy of pHluorin)
- Neuronal signaling (by live fluorescence imaging of FRET based biosensors for PKA, PKC etc)
- Nano-structures of receptor-Scaffold complexes and membrane structures (by super resolution microscopy and 'supported cell membrane sheets’)
- Protein-protein interactions (in vitro by fluorescence polarization, SPOT, ex vivo/in vivo by Co-IP and Bio-ID)
- Semi-high throughput screening (by fluorescence polarisation)
- Protein-lipid interactions (by flow cytometry and SPOT)