Techniques – University of Copenhagen

Techniques

In vitro:

  • Isolation and culturing of rat primary neurons (cortical, hippocampal, retinal, cerebellar granule neurons, motorneurons), astrocytes and microglia. Co-culture assays. Porcine glial cell cultures. Immortalized cell lines (U-87 MG glioblastoma).
  • Neurite outgrowth
  • Survival assays (H2O2 stress; kainic acid)
  • In vitro pro-inflammatory assay (microglia)
  • Slices

Molecular biology/biochemistry:

  • DNA/RNA purification, PCR, qPCR
  • transfections, sh/siRNA silencing
  • ELISA
  • MTS proliferation assay
  • In situ hybridization
  • Receptor autoradiography
  • GTPgammaS functional binding autoradiography

Imaging:

  • Light microscopy /stereological evaluation (CAST)
  • Fluorescence microscopy
  • Immunohistochemistry

In vivo:

  • 5-Choice serial reaction time task
  • Continuous performance test
  • Morris water maze
  • Social recognition test
  • Y-maze
  • Tail suspension test
  • Forced swim test
  • Elevated plus maze
  • Conditioned place preference
  • Surgery: i.c.v. injections, nerve crush, traumatic brain lesion, transcardial perfusion, CSF sample collection
  • Other animal models: seizure model (kainic acid, PTZ, electroconvulsive seizures), Alzheimer model, EAE, traumatic brain injury, 6-OHDA Parkinson model