Neurturin is a PGC-1α1-controlled myokine that promotes motor neuron recruitment and neuromuscular junction formation

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Neurturin is a PGC-1α1-controlled myokine that promotes motor neuron recruitment and neuromuscular junction formation. / Mills, Richard; Taylor-Weiner, Hermes; Correia, Jorge C; Agudelo, Leandro Z; Allodi, Ilary; Kolonelou, Christina; Martinez-Redondo, Vicente; Ferreira, Duarte M S; Nichterwitz, Susanne; Comley, Laura H; Lundin, Vanessa; Hedlund, Eva; Ruas, Jorge L; Teixeira, Ana I.

In: Molecular Metabolism, Vol. 7, 2018, p. 12-22.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Mills, R, Taylor-Weiner, H, Correia, JC, Agudelo, LZ, Allodi, I, Kolonelou, C, Martinez-Redondo, V, Ferreira, DMS, Nichterwitz, S, Comley, LH, Lundin, V, Hedlund, E, Ruas, JL & Teixeira, AI 2018, 'Neurturin is a PGC-1α1-controlled myokine that promotes motor neuron recruitment and neuromuscular junction formation', Molecular Metabolism, vol. 7, pp. 12-22. https://doi.org/10.1016/j.molmet.2017.11.001

APA

Mills, R., Taylor-Weiner, H., Correia, J. C., Agudelo, L. Z., Allodi, I., Kolonelou, C., Martinez-Redondo, V., Ferreira, D. M. S., Nichterwitz, S., Comley, L. H., Lundin, V., Hedlund, E., Ruas, J. L., & Teixeira, A. I. (2018). Neurturin is a PGC-1α1-controlled myokine that promotes motor neuron recruitment and neuromuscular junction formation. Molecular Metabolism, 7, 12-22. https://doi.org/10.1016/j.molmet.2017.11.001

Vancouver

Mills R, Taylor-Weiner H, Correia JC, Agudelo LZ, Allodi I, Kolonelou C et al. Neurturin is a PGC-1α1-controlled myokine that promotes motor neuron recruitment and neuromuscular junction formation. Molecular Metabolism. 2018;7:12-22. https://doi.org/10.1016/j.molmet.2017.11.001

Author

Mills, Richard ; Taylor-Weiner, Hermes ; Correia, Jorge C ; Agudelo, Leandro Z ; Allodi, Ilary ; Kolonelou, Christina ; Martinez-Redondo, Vicente ; Ferreira, Duarte M S ; Nichterwitz, Susanne ; Comley, Laura H ; Lundin, Vanessa ; Hedlund, Eva ; Ruas, Jorge L ; Teixeira, Ana I. / Neurturin is a PGC-1α1-controlled myokine that promotes motor neuron recruitment and neuromuscular junction formation. In: Molecular Metabolism. 2018 ; Vol. 7. pp. 12-22.

Bibtex

@article{682bd69d099f47e082389f360f56f611,

title = "Neurturin is a PGC-1α1-controlled myokine that promotes motor neuron recruitment and neuromuscular junction formation",

abstract = "OBJECTIVE: We examined whether skeletal muscle overexpression of PGC-1α1 or PGC-1α4 affected myokine secretion and neuromuscular junction (NMJ) formation.METHODS: A microfluidic device was used to model endocrine signaling and NMJ formation between primary mouse myoblast-derived myotubes and embryonic stem cell-derived motor neurons. Differences in hydrostatic pressure allowed for fluidic isolation of either cell type or unidirectional signaling in the fluid phase. Myotubes were transduced to overexpress PGC-1α1 or PGC-1α4, and myokine secretion was quantified using a proximity extension assay. Morphological and functional changes in NMJs were measured by fluorescent microscopy and by monitoring muscle contraction upon motor neuron stimulation.RESULTS: Skeletal muscle transduction with PGC-1α1, but not PGC-1α4, increased NMJ formation and size. PGC-1α1 increased muscle secretion of neurturin, which was sufficient and necessary for the effects of muscle PGC-1α1 on NMJ formation.CONCLUSIONS: Our findings indicate that neurturin is a mediator of PGC-1α1-dependent retrograde signaling from muscle to motor neurons.",

keywords = "Animals, Cells, Cultured, Mice, Motor Neurons/cytology, Mouse Embryonic Stem Cells/cytology, Myoblasts/cytology, Neural Stem Cells/cytology, Neurogenesis, Neuromuscular Junction/cytology, Neurturin/metabolism, Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha/metabolism, Synaptic Transmission",

author = "Richard Mills and Hermes Taylor-Weiner and Correia, {Jorge C} and Agudelo, {Leandro Z} and Ilary Allodi and Christina Kolonelou and Vicente Martinez-Redondo and Ferreira, {Duarte M S} and Susanne Nichterwitz and Comley, {Laura H} and Vanessa Lundin and Eva Hedlund and Ruas, {Jorge L} and Teixeira, {Ana I}",

year = "2018",

doi = "10.1016/j.molmet.2017.11.001",

language = "English",

volume = "7",

pages = "12--22",

journal = "Molecular Metabolism",

issn = "2212-8778",

publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Neurturin is a PGC-1α1-controlled myokine that promotes motor neuron recruitment and neuromuscular junction formation

AU - Mills, Richard

AU - Taylor-Weiner, Hermes

AU - Correia, Jorge C

AU - Agudelo, Leandro Z

AU - Allodi, Ilary

AU - Kolonelou, Christina

AU - Martinez-Redondo, Vicente

AU - Ferreira, Duarte M S

AU - Nichterwitz, Susanne

AU - Comley, Laura H

AU - Lundin, Vanessa

AU - Hedlund, Eva

AU - Ruas, Jorge L

AU - Teixeira, Ana I

PY - 2018

Y1 - 2018

N2 - OBJECTIVE: We examined whether skeletal muscle overexpression of PGC-1α1 or PGC-1α4 affected myokine secretion and neuromuscular junction (NMJ) formation.METHODS: A microfluidic device was used to model endocrine signaling and NMJ formation between primary mouse myoblast-derived myotubes and embryonic stem cell-derived motor neurons. Differences in hydrostatic pressure allowed for fluidic isolation of either cell type or unidirectional signaling in the fluid phase. Myotubes were transduced to overexpress PGC-1α1 or PGC-1α4, and myokine secretion was quantified using a proximity extension assay. Morphological and functional changes in NMJs were measured by fluorescent microscopy and by monitoring muscle contraction upon motor neuron stimulation.RESULTS: Skeletal muscle transduction with PGC-1α1, but not PGC-1α4, increased NMJ formation and size. PGC-1α1 increased muscle secretion of neurturin, which was sufficient and necessary for the effects of muscle PGC-1α1 on NMJ formation.CONCLUSIONS: Our findings indicate that neurturin is a mediator of PGC-1α1-dependent retrograde signaling from muscle to motor neurons.

AB - OBJECTIVE: We examined whether skeletal muscle overexpression of PGC-1α1 or PGC-1α4 affected myokine secretion and neuromuscular junction (NMJ) formation.METHODS: A microfluidic device was used to model endocrine signaling and NMJ formation between primary mouse myoblast-derived myotubes and embryonic stem cell-derived motor neurons. Differences in hydrostatic pressure allowed for fluidic isolation of either cell type or unidirectional signaling in the fluid phase. Myotubes were transduced to overexpress PGC-1α1 or PGC-1α4, and myokine secretion was quantified using a proximity extension assay. Morphological and functional changes in NMJs were measured by fluorescent microscopy and by monitoring muscle contraction upon motor neuron stimulation.RESULTS: Skeletal muscle transduction with PGC-1α1, but not PGC-1α4, increased NMJ formation and size. PGC-1α1 increased muscle secretion of neurturin, which was sufficient and necessary for the effects of muscle PGC-1α1 on NMJ formation.CONCLUSIONS: Our findings indicate that neurturin is a mediator of PGC-1α1-dependent retrograde signaling from muscle to motor neurons.

KW - Animals

KW - Cells, Cultured

KW - Mice

KW - Motor Neurons/cytology

KW - Mouse Embryonic Stem Cells/cytology

KW - Myoblasts/cytology

KW - Neural Stem Cells/cytology

KW - Neurogenesis

KW - Neuromuscular Junction/cytology

KW - Neurturin/metabolism

KW - Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha/metabolism

KW - Synaptic Transmission

U2 - 10.1016/j.molmet.2017.11.001

DO - 10.1016/j.molmet.2017.11.001

M3 - Journal article

C2 - 29157948

VL - 7

SP - 12

EP - 22

JO - Molecular Metabolism

JF - Molecular Metabolism

SN - 2212-8778

ER -

ID: 227431599

Department of Neuroscience