Techniques

  • Human pluripotent stem cells: Culturing and neural differentiation of human pluripotent stem cells to subtype-specific neural cells.
  • 3D stem cell models: Applying 3D models for neural differentiation of human stem cells into regionalised neural subtypes.
  • Microfluidic cell culturing: Development and use of microfluidic culturing devices to control regionalisation of human stem cells during differentiation
  • Proteomics: Shotgun proteomics (LC-MS/MS) for identifying proteins in cells and cell culture medium from human stem cells.
  • Molecular biology: Generation of CRISPR/Cas9 reporter cell lines, generation of lentiviral constructs, high-throughput qRT-PCR analysis
  • Bioinformatics: Single cell RNAsequencing using the 10X Chromium platform and lineage trajectory analysis by bioinformatics
  • In vivo transplantation: Rat models for stereotaxic xeno-transplantation of human neural cells
  • Imaging: Confocal fluorescence microscopy and immunohistochemistry