Sensing and regulation of cell volume - we know so much and yet understand so little: TRPV4 as a sensor of volume changes but possibly without a volume-regulatory role?
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Sensing and regulation of cell volume - we know so much and yet understand so little : TRPV4 as a sensor of volume changes but possibly without a volume-regulatory role? / Toft-Bertelsen, Trine L.; Larsen, Brian R.; MacAulay, Nanna.
In: Channels (Austin), Vol. 12, No. 1, 2018, p. 100-108.Research output: Contribution to journal › Review › peer-review
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TY - JOUR
T1 - Sensing and regulation of cell volume - we know so much and yet understand so little
T2 - TRPV4 as a sensor of volume changes but possibly without a volume-regulatory role?
AU - Toft-Bertelsen, Trine L.
AU - Larsen, Brian R.
AU - MacAulay, Nanna
PY - 2018
Y1 - 2018
N2 - Cellular volume changes lead to initiation of cell volume regulatory events, the molecular identity of which remains unresolved. We here discuss experimental challenges associated with investigation of volume regulation during application of large, non-physiological osmotic gradients. The TRPV4 ion channel responds to volume increase irrespectively of the molecular mechanism underlying cell swelling, and is thus considered a sensor of volume changes. Evidence pointing towards the involvement of TRPV4 in subsequent volume regulatory mechanisms is intriguing, yet far from conclusive. We here present an experimental setting with astrocytic cell swelling in the absence of externally applied osmotic gradients, and the lack of evidence for involvement of TRPV4 in this regulatory volume response. Our aim with these new data and the preceding discussion is to stimulate further experimental effort in this area of research to clarify the role of TRPV4 and other channels and transporters in regulatory volume responses.
AB - Cellular volume changes lead to initiation of cell volume regulatory events, the molecular identity of which remains unresolved. We here discuss experimental challenges associated with investigation of volume regulation during application of large, non-physiological osmotic gradients. The TRPV4 ion channel responds to volume increase irrespectively of the molecular mechanism underlying cell swelling, and is thus considered a sensor of volume changes. Evidence pointing towards the involvement of TRPV4 in subsequent volume regulatory mechanisms is intriguing, yet far from conclusive. We here present an experimental setting with astrocytic cell swelling in the absence of externally applied osmotic gradients, and the lack of evidence for involvement of TRPV4 in this regulatory volume response. Our aim with these new data and the preceding discussion is to stimulate further experimental effort in this area of research to clarify the role of TRPV4 and other channels and transporters in regulatory volume responses.
KW - aquaporins
KW - osmo-sensing
KW - volume regulation
KW - volume-sensitive ion channels
KW - transient receptor potential vanilloid 4 channel
KW - TRPV4
U2 - 10.1080/19336950.2018.1438009
DO - 10.1080/19336950.2018.1438009
M3 - Review
C2 - 29424275
VL - 12
SP - 100
EP - 108
JO - Channels
JF - Channels
SN - 1933-6950
IS - 1
ER -
ID: 197959846