Dynamic reprogramming of 5-hydroxymethylcytosine during early porcine embryogenesis

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Dynamic reprogramming of 5-hydroxymethylcytosine during early porcine embryogenesis. / Cao, Zubing; Zhou, Naru; Zhang, Yu; Zhang, Yuanliang; Wu, Ronghua; Li, Yunsheng; Zhang, Yunhai; Li, Ning.

In: Theriogenology, Vol. 81, No. 3, 2014, p. 496-508.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Cao, Z, Zhou, N, Zhang, Y, Zhang, Y, Wu, R, Li, Y, Zhang, Y & Li, N 2014, 'Dynamic reprogramming of 5-hydroxymethylcytosine during early porcine embryogenesis', Theriogenology, vol. 81, no. 3, pp. 496-508. https://doi.org/10.1016/j.theriogenology.2013.10.025

APA

Cao, Z., Zhou, N., Zhang, Y., Zhang, Y., Wu, R., Li, Y., Zhang, Y., & Li, N. (2014). Dynamic reprogramming of 5-hydroxymethylcytosine during early porcine embryogenesis. Theriogenology, 81(3), 496-508. https://doi.org/10.1016/j.theriogenology.2013.10.025

Vancouver

Cao Z, Zhou N, Zhang Y, Zhang Y, Wu R, Li Y et al. Dynamic reprogramming of 5-hydroxymethylcytosine during early porcine embryogenesis. Theriogenology. 2014;81(3):496-508. https://doi.org/10.1016/j.theriogenology.2013.10.025

Author

Cao, Zubing ; Zhou, Naru ; Zhang, Yu ; Zhang, Yuanliang ; Wu, Ronghua ; Li, Yunsheng ; Zhang, Yunhai ; Li, Ning. / Dynamic reprogramming of 5-hydroxymethylcytosine during early porcine embryogenesis. In: Theriogenology. 2014 ; Vol. 81, No. 3. pp. 496-508.

Bibtex

@article{82f8c90834ac428f925a3cbf9e0fbeb3,

title = "Dynamic reprogramming of 5-hydroxymethylcytosine during early porcine embryogenesis",

abstract = "DNA active demethylation is an important epigenetic phenomenon observed in porcine zygotes, yet its molecular origins are unknown. Our results show that 5-methylcytosine (5mC) converts into 5-hydroxymethylcytosine (5hmC) during the first cell cycle in porcine in vivo fertilization (IVV), IVF, and SCNT embryos, but not in parthenogenetically activated embryos. Expression of Ten-Eleven Translocation 1 (TET1) correlates with this conversion. Expression of 5mC gradually decreases until the morula stage; it is only expressed in the inner cell mass, but not trophectoderm regions of IVV and IVF blastocysts. Expression of 5mC in SCNT embryos is ectopically distinct from that observed in IVV and IVF embryos. In addition, 5hmC expression was similar to that of 5mC in IVV cleavage-stage embryos. Expression of 5hmC remained constant in IVF and SCNT embryos, and was evenly distributed among the inner cell mass and trophectoderm regions derived from IVV, IVF, and SCNT blastocysts. Ten-Eleven Translocation 3 was highly expressed in two-cell embryos, whereas TET1 and TET2 were highly expressed in blastocysts. These data suggest that TET1-catalyzed 5hmC may be involved in active DNA demethylation in porcine early embryos. In addition, 5mC, but not 5hmC, participates in the initial cell lineage specification in porcine IVV and IVF blastocysts. Last, SCNT embryos show aberrant 5mC and 5hmC expression during early porcine embryonic development.",

keywords = "5-Methylcytosine, Animals, Cytosine, DNA Methylation, Embryonic Development, Nuclear Transfer Techniques, Swine",

author = "Zubing Cao and Naru Zhou and Yu Zhang and Yuanliang Zhang and Ronghua Wu and Yunsheng Li and Yunhai Zhang and Ning Li",

year = "2014",

doi = "10.1016/j.theriogenology.2013.10.025",

language = "English",

volume = "81",

pages = "496--508",

journal = "Theriogenology",

issn = "0093-691X",

publisher = "Elsevier",

number = "3",

}

RIS

TY - JOUR

T1 - Dynamic reprogramming of 5-hydroxymethylcytosine during early porcine embryogenesis

AU - Cao, Zubing

AU - Zhou, Naru

AU - Zhang, Yu

AU - Zhang, Yuanliang

AU - Wu, Ronghua

AU - Li, Yunsheng

AU - Zhang, Yunhai

AU - Li, Ning

PY - 2014

Y1 - 2014

N2 - DNA active demethylation is an important epigenetic phenomenon observed in porcine zygotes, yet its molecular origins are unknown. Our results show that 5-methylcytosine (5mC) converts into 5-hydroxymethylcytosine (5hmC) during the first cell cycle in porcine in vivo fertilization (IVV), IVF, and SCNT embryos, but not in parthenogenetically activated embryos. Expression of Ten-Eleven Translocation 1 (TET1) correlates with this conversion. Expression of 5mC gradually decreases until the morula stage; it is only expressed in the inner cell mass, but not trophectoderm regions of IVV and IVF blastocysts. Expression of 5mC in SCNT embryos is ectopically distinct from that observed in IVV and IVF embryos. In addition, 5hmC expression was similar to that of 5mC in IVV cleavage-stage embryos. Expression of 5hmC remained constant in IVF and SCNT embryos, and was evenly distributed among the inner cell mass and trophectoderm regions derived from IVV, IVF, and SCNT blastocysts. Ten-Eleven Translocation 3 was highly expressed in two-cell embryos, whereas TET1 and TET2 were highly expressed in blastocysts. These data suggest that TET1-catalyzed 5hmC may be involved in active DNA demethylation in porcine early embryos. In addition, 5mC, but not 5hmC, participates in the initial cell lineage specification in porcine IVV and IVF blastocysts. Last, SCNT embryos show aberrant 5mC and 5hmC expression during early porcine embryonic development.

AB - DNA active demethylation is an important epigenetic phenomenon observed in porcine zygotes, yet its molecular origins are unknown. Our results show that 5-methylcytosine (5mC) converts into 5-hydroxymethylcytosine (5hmC) during the first cell cycle in porcine in vivo fertilization (IVV), IVF, and SCNT embryos, but not in parthenogenetically activated embryos. Expression of Ten-Eleven Translocation 1 (TET1) correlates with this conversion. Expression of 5mC gradually decreases until the morula stage; it is only expressed in the inner cell mass, but not trophectoderm regions of IVV and IVF blastocysts. Expression of 5mC in SCNT embryos is ectopically distinct from that observed in IVV and IVF embryos. In addition, 5hmC expression was similar to that of 5mC in IVV cleavage-stage embryos. Expression of 5hmC remained constant in IVF and SCNT embryos, and was evenly distributed among the inner cell mass and trophectoderm regions derived from IVV, IVF, and SCNT blastocysts. Ten-Eleven Translocation 3 was highly expressed in two-cell embryos, whereas TET1 and TET2 were highly expressed in blastocysts. These data suggest that TET1-catalyzed 5hmC may be involved in active DNA demethylation in porcine early embryos. In addition, 5mC, but not 5hmC, participates in the initial cell lineage specification in porcine IVV and IVF blastocysts. Last, SCNT embryos show aberrant 5mC and 5hmC expression during early porcine embryonic development.

KW - 5-Methylcytosine

KW - Animals

KW - Cytosine

KW - DNA Methylation

KW - Embryonic Development

KW - Nuclear Transfer Techniques

KW - Swine

U2 - 10.1016/j.theriogenology.2013.10.025

DO - 10.1016/j.theriogenology.2013.10.025

M3 - Journal article

C2 - 24315686

VL - 81

SP - 496

EP - 508

JO - Theriogenology

JF - Theriogenology

SN - 0093-691X

IS - 3

ER -

ID: 135279904

Department of Neuroscience