Cotransporter-mediated water transport underlying cerebrospinal fluid formation

Research output: Contribution to journal › Journal article › Research › peer-review

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Cotransporter-mediated water transport underlying cerebrospinal fluid formation. / Steffensen, Annette B.; Oernbo, Eva K.; Stoica, Anca; Gerkau, Niklas J.; Barbuskaite, Dagne; Tritsaris, Katerina; Rose, Christine R.; MacAulay, Nanna.

In: Nature Communications, Vol. 9, No. 1, 2167, 2018.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Steffensen, AB, Oernbo, EK, Stoica, A, Gerkau, NJ, Barbuskaite, D, Tritsaris, K, Rose, CR & MacAulay, N 2018, 'Cotransporter-mediated water transport underlying cerebrospinal fluid formation', Nature Communications, vol. 9, no. 1, 2167. https://doi.org/10.1038/s41467-018-04677-9

APA

Steffensen, A. B., Oernbo, E. K., Stoica, A., Gerkau, N. J., Barbuskaite, D., Tritsaris, K., Rose, C. R., & MacAulay, N. (2018). Cotransporter-mediated water transport underlying cerebrospinal fluid formation. Nature Communications, 9(1), [2167]. https://doi.org/10.1038/s41467-018-04677-9

Vancouver

Steffensen AB, Oernbo EK, Stoica A, Gerkau NJ, Barbuskaite D, Tritsaris K et al. Cotransporter-mediated water transport underlying cerebrospinal fluid formation. Nature Communications. 2018;9(1). 2167. https://doi.org/10.1038/s41467-018-04677-9

Author

Steffensen, Annette B. ; Oernbo, Eva K. ; Stoica, Anca ; Gerkau, Niklas J. ; Barbuskaite, Dagne ; Tritsaris, Katerina ; Rose, Christine R. ; MacAulay, Nanna. / Cotransporter-mediated water transport underlying cerebrospinal fluid formation. In: Nature Communications. 2018 ; Vol. 9, No. 1.

Bibtex

@article{f6e524d7a73a40229030a6544ad373e1,
title = "Cotransporter-mediated water transport underlying cerebrospinal fluid formation",
abstract = "Cerebrospinal fluid (CSF) production occurs at a rate of 500 ml per day in the adult human. Conventional osmotic forces do not suffice to support such production rate and the molecular mechanisms underlying this fluid production remain elusive. Using ex vivo choroid plexus live imaging and isotope flux in combination with in vivo CSF production determination in mice, we identify a key component in the CSF production machinery. The Na+/K+/2Cl- cotransporter (NKCC1) expressed in the luminal membrane of choroid plexus contributes approximately half of the CSF production, via its unusual outward transport direction and its unique ability to directly couple water transport to ion translocation. We thereby establish the concept of cotransport of water as a missing link in the search for molecular pathways sustaining CSF production and redefine the current model of this pivotal physiological process. Our results provide a rational pharmacological target for pathologies involving disturbed brain fluid dynamics.",
author = "Steffensen, {Annette B.} and Oernbo, {Eva K.} and Anca Stoica and Gerkau, {Niklas J.} and Dagne Barbuskaite and Katerina Tritsaris and Rose, {Christine R.} and Nanna MacAulay",
year = "2018",
doi = "10.1038/s41467-018-04677-9",
language = "English",
volume = "9",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "nature publishing group",
number = "1",

}

RIS

TY - JOUR

T1 - Cotransporter-mediated water transport underlying cerebrospinal fluid formation

AU - Steffensen, Annette B.

AU - Oernbo, Eva K.

AU - Stoica, Anca

AU - Gerkau, Niklas J.

AU - Barbuskaite, Dagne

AU - Tritsaris, Katerina

AU - Rose, Christine R.

AU - MacAulay, Nanna

PY - 2018

Y1 - 2018

N2 - Cerebrospinal fluid (CSF) production occurs at a rate of 500 ml per day in the adult human. Conventional osmotic forces do not suffice to support such production rate and the molecular mechanisms underlying this fluid production remain elusive. Using ex vivo choroid plexus live imaging and isotope flux in combination with in vivo CSF production determination in mice, we identify a key component in the CSF production machinery. The Na+/K+/2Cl- cotransporter (NKCC1) expressed in the luminal membrane of choroid plexus contributes approximately half of the CSF production, via its unusual outward transport direction and its unique ability to directly couple water transport to ion translocation. We thereby establish the concept of cotransport of water as a missing link in the search for molecular pathways sustaining CSF production and redefine the current model of this pivotal physiological process. Our results provide a rational pharmacological target for pathologies involving disturbed brain fluid dynamics.

AB - Cerebrospinal fluid (CSF) production occurs at a rate of 500 ml per day in the adult human. Conventional osmotic forces do not suffice to support such production rate and the molecular mechanisms underlying this fluid production remain elusive. Using ex vivo choroid plexus live imaging and isotope flux in combination with in vivo CSF production determination in mice, we identify a key component in the CSF production machinery. The Na+/K+/2Cl- cotransporter (NKCC1) expressed in the luminal membrane of choroid plexus contributes approximately half of the CSF production, via its unusual outward transport direction and its unique ability to directly couple water transport to ion translocation. We thereby establish the concept of cotransport of water as a missing link in the search for molecular pathways sustaining CSF production and redefine the current model of this pivotal physiological process. Our results provide a rational pharmacological target for pathologies involving disturbed brain fluid dynamics.

U2 - 10.1038/s41467-018-04677-9

DO - 10.1038/s41467-018-04677-9

M3 - Journal article

C2 - 29867199

AN - SCOPUS:85048131386

VL - 9

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

IS - 1

M1 - 2167

ER -

ID: 197959338