MacAulay Lab

The research group focuses on elucidation of the molecular mechanisms underlying water and ion homeostasis in the mammalian brain under both physiological and pathophysiological conditions.

Nanna MacAulay info

 

Idiopathic intracranial hypertension – IIH. That means elevated pressure inside the skull of unknown origin. This disease mainly occurs in young obese females but we do not know the link between elevated body mass and elevated intracranial pressure  – and therefore have no targeted pharmacological treatment options 💊.

From our animal studies 🐀 (see the science poetry below), it appears that obesity in itself does not promote IIH, and that the diet - on which the rats become obese - makes a difference (healthy food 🍅🥒; no increase in intracranial pressure (PMID: 38273331, see science poetry below), ‘Western diet’ 🍔 🍟; increase in intracranial pressure (PMID: 37328884)).

Mimicking the elevated brain fluid testosterone observed in female IIH patients (PMID: 30753168) in obese experimental rats, we observed an increased brain fluid secretion rate 💦 , which may well contribute to the elevated brain fluid content and intracranial pressure also in IIH patients 🏥 .

We must understand brain fluid dynamics in physiology to be able to control it in pathology – and we keep working towards just that in the MacAulay lab.

Thanks 🙏 to all contributing colleagues 💪, first an foremost Jonathan Wardman, but also experimental physiologists Trine L. Toft, Mette Nyholm Jensen, bioinformatician Søren Norge Andreassen, engineer Jens E. Wilhjelm, neuroophthalmologists Steffen Hamann and Steffen Heegaard, pharmacologist Bjarne Styrishave, and neurologist/IIH expert Alexandra Sinclair, and to Lundbeckfonden / Lundbeck Foundation for supporting this study 💰. We couldn’t do it without you.

 

 

Choroid plexus secretes our brain fluids, the cerebrospinal fluid, and – at the same time; keeps the brain potassium concentration low to prevent hyperexcitability. An amazing structure!

Thanks to Alex Verkrhatsky, Christine Rose, Maiken Nedergaard and Verena Unviet for the invitation to write a review on ion transport in this intriguing tissue for their special issue in Cell Calcium

on ‘Ionic signalling (beyond calcium) in the nervous system: Physiology and Pathophysiology’ and to Trine Toft-Bertelsen to join me in the effort.

 

 

The short answer: We don’t know.

To reduce brain fluid content – and thus elevated intracranial pressure – in this patient group, we must know how and why the fluid accumulates in the brain.

That is our mission.

Thanks to Sara Diana Lolansen, Shai Ben-Shoshan, and Tiit Mathiesen for their thorough work on this important piece, and to the Novo Nordisk Foundation and IMK Almene Fond for funding our research on hydrocephalus.

To read the full review: https://rdcu.be/dkIBK

 

Hydrocephalus – water in the head – may arise after a hemorrhagic event. It has been speculated whether this brain water accumulation occurs with an increased osmotic pressure due to the blood entrance into the ventricular system.

It does not!

Read the short version of our new study on CSF osmolality in patients with subarachnoid haemorrhage on verse below or find the link to the publication below in the commentaries. 

 

 

 

It is so odd – one can have elevated intracranial pressure without enlarged ventricles (hydrocephalus) as in idiopathic intracranial hypertension (IIH) and – oppositely; one can have enlarged ventricles without elevated intracranial pressure as in idiopathic normal pressure hydrocephalus (iNPH). And we do not know why. 

Spontaneously hypertensive rats (SHR) develop enlarge ventricles starting around week seven after birth. Here we show that their hydrocephalus comes with elevated brain fluid content but arises without changes in their intracranial pressure (ICP), their rate of cerebrospinal fluid (CSF) secretion, or their resistance to CSF drainage. Read the full study here: https://rdcu.be/df4bn or get the gist from the attached poem J.

The search for the molecular drivers of the various forms of hydrocephalus formation continues. Thanks to Sara Lolansen, Dagne Barbuskaite, Richard Keep and all other collaborators for their valuable contribution and to the Novo Nordic Foundation for their support of our research on hydrocephalus.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

  • Sara D. Lolansen, Nina Rostgaard, Tenna Capion, Nicolas H. Nørager, Markus H. Olsen, Marianne Juhler, Tiit I. Mathiesen & Nanna MacAulay (2023) Posthemorrhagic hydrocephalus in patients with subarachnoid hemorrhage occurs independently of CSF osmolality. International Journal of Molecular Science 24:11476
  • Anette B. Steffensen, Beatriche L. Edelbo, Dagne Barbuskaite, Søren N. Andreassen, Markus H. Olsen, Kirsten Møller & Nanna MacAulay (2023) Nocturnal increase in cerebrospinal fluid secretion as a circadian regulator of intracranial pressure. Fluids and Barriers of the CNS 20:49
  • Jonathan H. Wardman, Mette Nyholm Jensen, Søren Norge Andreassen, Bjarne Styrishave, Jens E. Wilhjelm, Alexandra J. Sinclair & Nanna MacAulay (2023) Modelling idiopathic intracranial hypertension in rats: contributions of high fat diet and testosterone to intracranial pressure and cerebrospinal fluid production. Fluids and Barriers of the CNS 20:44
  • Trine L Toft-Bertelsen, Dagne Barbuskaite,……, Marianne Juhler & Nanna MacAulay (2022) Lysophosphatidic acid as a CSF lipid in posthemorrhagic hydrocephalus that drives CSF accumulation via TRPV4-induced hyperactivation of NKCC1. Fluids and Barriers of the CNS 19:69
  • Eva K. Oernbo, Annette B. Steffensen, ……, Christine R. Rose, Vartan Kurtcuoglu & Nanna MacAulay (2022) Membrane transporters control cerebrospinal fluid formation independently of conventional osmosis to modulate intracranial pressure. Fluids and Barriers of the CNS 19:65
  • Søren N. Andreassen, Trine L. Toft-Bertelsen, Jonathan H. Wardman, Rene Villadsen & Nanna MacAulay (2022) Transcriptional profiling of transport mechanisms and regulatory pathways in rat choroid plexus. Fluids and Barriers of the CNS 19:44
  • Nanna MacAulay (2021) Molecular mechanisms of brain water transport. Nature Reviews Neuroscience 6:326-344
  • Annette B. Steffensen, Eva K. Oernbo, Anca Stoica, Niklas J. Gerkau, Dagne Barbuskaite, Katerina Tritsaris, Christine R. Rose & Nanna MacAulay (2018) Cotransporter-mediated water transport underlying cerebrospinal fluid formation. Nature Communications 9:2167
  • Brian R. Larsen & Nanna MacAulay (2017) Activity-dependent astrocyte swelling is mediated by pH-regulating mechanisms. GLIA 65:1668-1681
  • Annette B. Steffensen, Jeremy Sword, Debbie Croom, Sergei A. Kirov & Nanna MacAulay (2015) Cotransporters as a molecular mechanism underlying spreading depolarization-induced dendritic beading. Journal of Neuroscience 35:12172-12187
  • Brian R. Larsen, Mette Assentoft, M.L. Cotrina, Susan Z. Hua, Maiken Nedergaard, Kai Kaila, Juha Voipio & Nanna MacAulay(2014) Contributions of the Na+/K+-ATPase, NKCC1, and Kir4.1 to hippocampal K+-clearance and volume responses. GLIA 62:608-622
  • Toft-Bertelsen TL, Krízaj D & MacAulay N (2017) When size matters: transient receptor potential vanilloid 4 channel as a volume-sensor rather than an osmo-sensor. Journal of Physiology 595:3287-3302
  • Steffensen AB, Sword J, Croom D, Kirov SA & MacAulay N (2015) Cotransporters as a molecular mechanism underlying spreading depolarization-induced dendritic beading. Journal of Neuroscience 35:12172-12187
  • Larsen BR, Assentoft A, Cotrina ML, Hua SZ, Nedergaard M, Kaila K, Voipio J & MacAulay N (2014) Contributions of the Na+/K+-ATPase, NKCC1, and Kir4.1 to hippocampal K+-clearance and volume responses. GLIA 62:608-622

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Born 15th December 1972 

Education

2020                DMSci, Faculty of Health Sciences, UCPH
2002                PhD, Faculty of Health Sciences, UCPH
1998                MSc in biochemistry, McGill University, Canada
1995-96          Student exchange to McGill University, Canada
1995                BSc in biology, UCPH

Positions

2019-               Professor at Department of Neuroscience UCPH
2015-2018      Associate professor at Department of Neuroscience UCPH
2006-2015      Associate professor at Department of Cellular and Molecular Medicine, UCPH
2004-2006      Associate professor at Department of Medical Physiology, UCPH
2002-2003      Postdoc at Department of Medical Physiology, UCPH

Prizes and Awards

2023                Lundbeck Foundation’s Scientific Enrichment Prize
2023                Fondsbørsvekselerer H Hansen og hustrus scholarship
2020                Nominated for DFF’s Elite Researcher (as one out of eight from UCPH)
2019                Leo Dannin’s Research prize
2012                DFF’s Starting Grant award ‘Sapere Aude’
2008                L’Oreal, Unesco and the Royal Danish Academy of Sciences’ award
2006                Danish Independent Research Council’s award ’Young Elite Researcher’
1996                 Scholarship from Medical Research Council of Canada, McGill University

Funding ID (since 2011 only)

2024-2030       Lundbeck Foundation Professorship: 20 MDKK
2022-2025       Friis Foundation: 0.5 MDKK
2021-2024       Michaelsen’s Foundation: 0.5 MDKK
2020-2023      Independent Research Fund Denmark: 2.5 MDKK
2020-2024      Lundbeck Foundation Ascending investigator grant: 5 MDKK
2020-2021      Carlsberg Foundation: 0.3 MDKK
2019-2023      Lundbeck Foundation Thematic Grant (co-applicant, my share): 4.4 MDKK
2019-2021      NovoNordisk Foundation Symposium: 0.25 MDKK
2018-2022      IMK Almene Foundation: 0.9 MDKK
2018-2022      Friis’ Foundation: 0.5 MDKK
2018-2019      Brdr. Hartmann’s Foundation: 0.5 MDKK
2017-2022      NovoNordisk Foundation – Tandem (main applicant, my share): 7.5 MDKK
2019-2020      National Institute of Health (co-investigator, my share): 0.15 MDKK
2016-2018      NovoNordisk Foundation: 1.2 MDKK
2016-2019      Augustinus Foundation: 0.3 MDKK
2016-2017      Michaelsen’s Foundation: 0.3 MDKK
2016-2017      Carslberg Foundation: 0.1 MDKK
2014-2017      Lundbeck Foundation: 1.6 MDKK
2014-2015      Friis’ Foundation: 0.3 MDKK
2013-2016      Thorberg’s Foundation: 2.5 MDKK
2012-2017      Independent Research Fund Denmark (Sapere Aude): 6.8 MDKK
2011-2014      Independent Research Fund Denmark 1.8 MDKK
2011-2014      Lundbeck Foundation: 0.7 MDKK

Commissions of trust and Meeting organization

2023 –             International Brain Barriers Society steering council member
2022                Co-organiser of Matchpoints – our fabulous brain, Aarhus University
2021 – 2022    Reviewer for the Portuguese Research Council
2021                BrainH2O Symposium, UCPH
2019 –             Board member Jakobinerklubben
2019 – 2022   Panel member British Royal Society International Exchanges
2019 –            Associate Editor, Fluids and Barriers in the CNS
                        (editorial board member 2016-)
2017 – 2020   Member of Department of Neuroscience management team
2016 –            Head of the Graduate Program in Neuroscience (NeuroGrad), UCPH
2016 –            Organizer of the Neuroseminar series, Department of Neuroscience
2016               BrainWater Minisymposium, UCPH
2016               Scientific board of ‘the 19th Int. Symposium on Signal Transd. at the BBBs’
2013 –            Editorial Board Member, GLIA
2013 – 2016   Co-chair of the Society of Physiology’s special interest group
                       “Molecular physiology of channels and transporters’
2011 – 2016   Strategic research committee at the Faculty of Health, UCPH
2008 – 2015   Departmental research committee, Dept of Cell and Mol Med, UCPH
2008 – 2015    SPS ‘Scand. Conference on Mol. Physiol. of Channels and Transporters’, SE

 

 

 

 

 

 

 

 

In vivo animal experimentation on anesthetized rodents (determination of CSF production rate, intracranial pressure, CSF outflow resistance, brain water content, etc.)

Two-electrode voltage clamp and volume measurement of Xenopus oocytes (heterologously expressing transport proteins)

Radio-isotope flux measurements (in ex vivo tissue, cell culture, Xenopus oocytes)

Molecular biology (plasmid preparation, mutagenesis, subcloning, sequencing, in vitro RNA transcription, etc.)

Western blot, surface biotinylation, immunostaining, etc.

 

 

2024

107. Jonathan H. Wardman, Søren N Andreassen, Trine L. Toft-Bertelsen, Mette N Jensen, Jens E Wilhjelm, Bjarne Styrishave, Steffen Hamann, Steffen Heegaard, Alexandra J Sinclair A & NannaMacAulay (2024) CSF hyperdynamics in rats mimicking the obesity and androgen excess characteristic of patients with idiopathic intracranial hypertension. Fluids Barriers CNS 21(1):10

106. Michael Thormann, N Traube, N Yehia, R Koestler, G Galabova, NannaMacAulay, Trine L Toft-Bertelsen (2024) Toward New AQP4 Inhibitors: ORI-TRN-002. International Journal of Molecular Sciences 25(2):924

2023

105. Nina Rostgaard, Markus H. Olsen, Sara D. Lolansen, Nicolas H. Nørager, Peter Plomgaard, Nanna MacAulay & Marianne Juhler (2023) Ventricular CSF proteomic profiles and predictors of surgical treatment outcome in chronic hydrocephalus. Acta Neurochirurgica, 165(12):4059-4070

104. Nanna MacAulay & Trine L. Toft-Bertelsen (2023) Dual function of the choroid plexus: cerebrospinal fluid production and control of brain ion homeostasis. Cell Calcium, 116:102797

103. Trine L. Toft-Bertelsen, Søren N. Andreassen, Nina Rostgaard, Markus H. Olsen, Nicolas H. Nørager, Tenna Capion, Marianne Juhler & Nanna MacAulay (2023) Distinct cerebrospinal fluid lipid signature in patients with subarachnoid hemorrhage-induced hydrocephalus. Biomedicines 11:2360

102. Shai D. Ben-Shoshan, Sara D. Lolansen, Tiit I. Mathiesen & Nanna MacAulay (2023) CSF hypersecretion versus impaired CSF absorption in posthemorrhagic hydrocephalus: a systematic review. Acta Neurochirurgica, online ahead of print

101. Beatriche L. Edelbo, Søren N. Andreassen, Annette B. Steffensen & Nanna MacAulay (2023) Day-night fluctuations in choroid plexus transcriptomics and cerebrospinal fluid metabolomics. Proceedings of the National Academy of Sciences U.S.A. Nexus 2:pgad262

100. Connar S.J. Westgate, Christina Kamp-Jensen, Ida M. E. Israelsen, Trine Toft-Bertelsen, Jonathan H. Wardman, Christian A. Jensen, Bjarne Styrishave, Nanna MacAulay, Rigmor H. Jensen, Sajedeh Eftekhari (2023) Acetazolamide and topiramate lower intracranial pressure through different mechanisms: the effect of acute and chronic administration. British Journal of Pharmacology, online ahead of print

99. Sara D. Lolansen, Nina Rostgaard, Tenna Capion, Nicolas H. Nørager, Markus H. Olsen, Marianne Juhler, Tiit I. Mathiesen & Nanna MacAulay (2023) Posthemorrhagic hydrocephalus in patients with subarachnoid hemorrhage occurs independently of CSF osmolality. International Journal of Molecular Science 24:11476

98. Sara D. Lolansen, Dagne Barbuskaite, Fenghui Ye, Jianming Xiang, Richard Keep & Nanna MacAulay (2023) Spontaneously hypertensive rats can become hydrocephalic despite undisturbed secretion and drainage of cerebrospinal fluid. Fluids and Barriers of the CNS 20:53

97. Anette B. Steffensen, Beatriche L. Edelbo, Dagne Barbuskaite, Søren N. Andreassen, Markus H. Olsen, Kirsten Møller & Nanna MacAulay (2023) Nocturnal increase in cerebrospinal fluid secretion as a circadian regulator of intracranial pressure. Fluids and Barriers of the CNS 20:49

96. Jonathan H. Wardman, Mette Nyholm Jensen, Søren Norge Andreassen, Bjarne Styrishave, Jens E. Wilhjelm, Alexandra J. Sinclair & Nanna MacAulay (2023) Modelling idiopathic intracranial hypertension in rats: contributions of high fat diet and testosterone to intracranial pressure and cerebrospinal fluid production. Fluids and Barriers of the CNS 20:44

95. Nina Rostgaard, Markus H. Olsen, Tenna Capion, Nanna MacAulay & Marianne Juhler (2023) Inflammatory markers as predictors of shunt dependency and functional outcome in patients with aneurysmal subarachnoid hemorrhage. Biomedicines 11:997

94. Pooya R. Khamesi, V. Charitatos, Eva K. Heerfordt, Nanna MacAulay & Vartan Kurtcuoglu (2023) Are standing osmotic gradients the main driver of cerebrospinal fluid production? A computational analysis. Fluids and Barriers of the CNS 20:18

93. Nina Rostgaard, Markus H. Olsen, M. Ottenheijm, L. Drici, Anja H. Simonsen, Per Plomgaard, Hanne Gredal, Helle H. Poulsen, Henrik Zetterberg, Kaj Blennow, Steen G. Hasselbalch, Nanna MacAulay & Marianne Juhler (2023) Differential proteomic profile of lumbar and ventricular cerebrospinal fluid. Fluids and Barriers of the CNS 20:6

2022

92. Trine L Toft-Bertelsen, Dagne Barbuskaite, Eva K Heerfordt, Sara D Lolansen, Søren N Andreassen, Nina Rostgaard, Markus H Olsen, Nicolas H. Norager, Tenna Capion, Martin F Rath, Marianne Juhler & Nanna MacAulay (2022) Lysophosphatidic acid as a CSF lipid in posthemorrhagic hydrocephalus that drives CSF accumulation via TRPV4-induced hyperactivation of NKCC1. Fluids and Barriers of the CNS 19:69

91. Eva K. Oernbo, Annette B. Steffensen, Pooya R Khamesi, Trine L Toft-Bertelsen, Dagne Barbuskaite, Frederik Vilhardt, Niklas J. Gerkau, Katerina Tritsaris, Anja H Simonsen, Sara D Lolansen, Søren N Andreassen, Steen G. Hasselbalch, Thomas Zeuthen, Christine R. Rose, Vartan Kurtcuoglu & Nanna MacAulay (2022) Membrane transporters control cerebrospinal fluid formation independently of conventional osmosis to modulate intracranial pressure. Fluids and Barriers of the CNS 19:65

90. Sara D. Lolansen, Nina Rostgaard, Dagne Barbuskaite, Tenna Capion, Markus H. Olsen, Nicolas H. Norager, Frederik Vilhardt, Søren N. Andreassen, Trine L. Toft-Bertelsen, F. Ye, Marianne Juhler, Richard Keep & Nanna MacAulay (2022) Posthemorrhagic hydrocephalus associates with elevated inflammation and CSF hypersecretion via activation of choroidal transporters. Fluids and Barriers of the CNS 19:62

89. Dagne Barbuskaite, Eva K. Oernbo, Jonathan H. Wardman, Trine L. Toft-Bertelsen, Eller Conti, Søren N. Andreassen, Niklas J. Gerkau, Christine R. Rose & Nanna MacAulay (2022) Acetazolamide modulates intracranial pressure directly by its action on the cerebrospinal fluid secretion apparatus. Fluids and Barriers of the CNS 19:53

88. Eva K.Oernbo, Annette B. Steffensen, Hanne Gredal, Helle H. Poulsen, Nina Rostgaard, CH Rasmussen, M Møller-Nissen, Anja H. Simonsen, Steen G. Hasselbalch, Marianne Juhler & Nanna MacAulay (2022) Cerebrospinal fluid osmolality cannot predict development or surgical outcome of idiopathic normal pressure hydrocephalus. Fluids and Barriers of the CNS 19:52

87. Søren N. Andreassen, Trine L. Toft-Bertelsen, Jonathan H. Wardman, Rene Villadsen & Nanna MacAulay (2022) Transcriptional profiling of transport mechanisms and regulatory pathways in rat choroid plexus. Fluids and Barriers of the CNS 19:44

86. Marianne Juhler, TS Hansen, HVG Novrup, Nanna MacAulay & Tina N. Munch (2022) Hydrocephalus study design: testing new hypotheses in clinical studies and bench-to-bedside research. World Neurosurgery 161:424-431

85. Nanna MacAulay, Richard F. Keep & Thomas Zeuthen (2022) Cerebrospinal fluid production by the choroid plexus: a century of barrier research revisited. Fluids and Barriers of the CNS 19:26

2021

84. Sara Diana Lolansen, Nina Rostgaard,Søren Norge Andreassen, Anja Hviid Simonsen,Marianne Juhler, Steen Gregers Hasselbalch Nanna MacAulay (2021) Elevated CSF inflammatory markers in patients with idiopathic normal pressure hydrocephalus do not promote NKCC1 hyperactivity in rat choroid plexus. Fluids and Barriers of CNS 18:54

83. Monica Lakk, GF Hoffmann, A. Gorusupudi, E Enyong, A Lin, PS Bernstein, Trine L. Toft-Bertelsen, Nanna MacAulay, MH Elliott, David Križaj (2021) Membrane cholesterol regulates TRPV4 function, cytoskeletal expression, and the cellular response to tension. Journal of Lipid Research 62:100145

82. Trine L. Toft-Bertelsen & Nanna MacAulay (2021) TRPing on cell swelling - TRPV4 senses it.  Frontiers of Immunology 12:730982

81. Nanna MacAulay (2021) Reply to ‘Aquaporin 4 and glymphatic flow have central roles in brain fluid homeostasis’. Nature Reviews Neuroscience 10:651-652

80. Nanna MacAulay (2021) Molecular mechanisms of brain water transport. Nature Reviews Neuroscience 6:326-344

79. Sara D. Lolansen, Nina Rostgaard, Eva Kjer Oernbo, Marianne Juhler, Anja Hviid Simonsen & Nanna MacAulay (2021). Inflammatory markers in cerebrospinal fluid from patients with hydrocephalus: a systematic literature review. Disease Markers 2021:8834822

78. Trine L. Toft-Bertelsen & Nanna MacAulay (2021) TRPing to the point of clarity: understanding the function of the complex TRPV4 ion channel. Cells 10:165

2020

77. G.V. Mkrtchyan, K. Abdelmohsen, P. Andreux,... ...Nanna MacAulay... ... et al. (2020) ARDD 2020: from aging mechanisms to interventions. Aging 12:24484-24503

76. Nanna MacAulay & Christine C. Rose (2020) Rebuttal from Nanna MacAulay & Christine C. Rose. Journal of Physiology 598:4743

75. Nanna MacAulay & Christine C. Rose (2020) CrossTalk opposing view: NKCC1 in the luminal membrane of choroid plexus is outwardly directed under basal conditions and contributes directly to cerebrospinal fluid secretion. Journal of Physiology 598: 4737-4739

74. Trine Lisberg Toft-Bertelsen, Brian Roland Larsen, Sofie Kjellerup Christensen, Himanshu Khandelia, Helle S. Waagepetersen & Nanna MacAulay (2020) Clearance of activity-evoked K+ transients and associated glia cell swelling occur independently of AQP4; a study with an isoform-selective AQP4 inhibitor. GLIA 69: 28-41

73. Nanna MacAulay (2020) Molecular mechanisms of K+ clearance and extracellular space shrinkage-Glia cells as the stars. GLIA 68:2192-2211

72. Brian Skriver Nielsen, Trine Lisberg Toft-Bertelsen, Sara D. Lolansen, Connor L. Anderson, Morten Schak Nielsen, Roger J. Thompson & Nanna MacAulay (2020) Pannexin 1 activation and inhibition is permeant-selective. Journal of Physiology 598:361-379

2019

71. Trine Lisberg Toft-Bertelsen, Oleg Yarishkin, Sara Redmon,Tam TT Phuong, David Krizaj & Nanna MacAulay (2019) Volume sensing in the transient receptor potential vanilloid 4 ion channel is cell type-specific and mediated by an N-terminus volume-sensing domain. Journal of Biological Chemistry 294:18421-18434

70. Brian Skriver Nielsen, Francesco Zonta, Thomas Farkas, Thomas Litman, Morten Schak Nielsen & Nanna MacAulay (2019) Structural determinants underlying permeant discrimination of the Cx43 hemichannel. Journal of Biological Chemistry 294:16789-16803

69. Kasper Lykke, Mette Assentoft, Sofie Hørlyck, Hans Christian C. Helms, Anca Stoica, Trine L. Toft-Bertelsen, Katerina Tritsaris, Frederik Vilhardt, Birger Brodin & Nanna MacAulay (2019) Evaluating the involvement of cerebralmicrovascular endothelial Na+/K+-ATPase and Na+-K+-2Cl- co-transporter (NKCC1) in electrolyte fluxes in an in vitro BBB model of dehydration. Journal of Cerebral Blood Flow and Metabolism 39:497-512

68. Brian Roland Larsen, Anca Stoica, Nanna MacAulay (2019) Developmental maturation of activity-induced K+ and pH transients and the associated extracellular space dynamics in the rat hippocampus. Journal of Physiology 597:583-597

2018

67. Claudia Brandt, P Seja, Katrin Töllner, Kerstin Römermann, Philip Hampel, M Kalesse, A Kipper, Peter W Feit, Kasper Lykke, Trine L Toft-Bertelsen, P Paavilainen, I Spoljaric, Martin Puskarjov , Nanna MacAulay, Kai Kaila, Wolfgang Löscher (2018) Bumepamine, a brain-permeant benzylamine derivative of bumetanide, does not inhibit NKCC1 but is more potent to enhance phenobarbital's anti-seizure efficacy. Neuropharmacology 143:186-204

66. Eva Kjer Oernbo, Kasper Lykke, Annette B. Steffensen, Kathrin Töllner, Christina Kruuse, Martin Fredensborg Rath, Wolfgang Löscher & Nanna MacAulay (2018) Cerebral influx of Na+ and Cl- as the osmotherapy-mediated rebound response in rats. Fluids and Barriers of the CNS 15:27

65. Philip Hampel, Kerstin Römermann, Nanna MacAulay & Wolfgang Löscher (2018) Azosemide is more potent than bumetanide and various other loop diuretics to inhibit the sodium-potassium-chloride-cotransporter human variants hNKCC1A and hNKCC1B. Scientific Reports 8:9877

64. Annette B. Steffensen, Eva K. Oernbo,Anca Stoica, Niklas J. Gerkau, Dagne Barbuskaite, Katerina Tritsaris, Christine R. Rose & Nanna MacAulay (2018) Cotransporter-mediated water transport underlying cerebrospinal fluid formation. Nature Communications 9:2167

63. Katja Stahl, S. Rahmani, A. Prydz, N. Skauli, Nanna MacAulay, M.N. Mylonakou, R. Torp, Ø. Skare, T. Berg, T.B. Leergaard, R.E. Paulsen, O.P. Ottersen, Mahmood Amiry-Moghaddam (2018) Targeted deletion of the aquaglyceroporin AQP9 is protective in a mouse model of Parkinson's disease. PLoS One 13:e0194896

62. Trine L. Toft-Bertelsen, Brian Roland Larsen & NannaMacAulay (2018) Sensing and regulation of cell volume - we know so much and yet understand so little: TRPV4 as a sensor of volume changes but possibly without a volume-regulatory role? Channels 12;100-108

61. Siren Berland, Trine L. Toft-Bertelsen, Ingvild Aukrust, Jan Byska, Marc Vaudel, Laurence A. Bindoff, Nanna MacAulay (shared senior authorship) & Gunnar Houge (2018)A denovo Ser111Thr variant in aquaporin-4 in a patient with intellectual disability, transient signs of brain ischemia, transient cardiac hypertrophy, and progressive gait disturbance. Molecular Case Studies 4, pii: a002303

2017

60. Brian Skriver Nielsen, Jette Skov Alstrom,Bruce J. Nicholson, Morten Schak Nielsen & Nanna MacAulay (2017)Permeant-specific gating of connexin 30 hemichannels. Journal of Biological Chemistry 292:19999-20009

59. Lena Rosenbaek, Federica Rizzo, Qi Wu, Lorena Rojas-Vega, Gerardo Gamba Ayala, Nanna MacAulay, Oliver Staub & Robert A. Fenton (2017) The thiazide sensitive sodium chloride co-transporter NCC is modulated by site-specific ubiquitylation. Scientific Reports 7:12981

58. Anca Stoica, Brian Roland Larsen, Mette Assentoft, Rikke Holm, Leanne M. Holt, Frederik Vilhardt, Bente Vilsen, Karin Lykke-Hartmann, Michelle L. Olsen & Nanna MacAulay (2017) The α2β2 isoform combination dominates the astrocytic Na+/K+-ATPase activity and is rendered nonfunctional by the α2.G301R familial hemiplegic migraine type 2-associated mutation. GLIA 65:1777-1793

57. Brian Roland Larsen & Nanna MacAulay (2017) Activity-dependent astrocyte swelling is mediated by pH-regulating mechanisms. GLIA 65:1668-1681

56. Lena L. Rosenbaek. F. Rizzo, Nanna MacAulay, Olivier Staub & Robert A. Fenton (2017) Functional assessment of sodium chloride co-transporter NCC mutants in polarized mammalian epithelial cells. American Journal of Physiology - Renal Physiology 313:495-504

55. Brian s. Nielsen, Daniel B. Hansen, Bruce R. Ransom, Morten S. Nielsen & Nanna MacAulay (2017) Connexin hemichannels in astrocytes: an assessment of controversies regarding their functional characteristics. Neurochemical Research 42:2537-2550

54. Trine L. Toft-Bertelsen, David Krízaj & Nanna MacAulay (2017) When size matters: transient receptor potential vanilloid 4 channel as a volume-sensor rather than an osmo-sensor. Journal of Physiology 595:3287-3302

2016

53. Emma Olesen, Hanne Moeller, Mette Assentoft, Nanna MacAulay & Robert Fenton (2016) The vasopressin type-2 receptor and prostaglandin receptors EP2 and EP4 can increase aquaporin-2 plasma membrane targeting through a cAMP independent pathway. American Journal of Physiology – Renal Physiology 311:935-944

52. Mette Assentoft, Shreyas Kaptan, Hans-Peter Schneider, Joachim W. Deitmer, Bert L. de Groot & Nanna MacAulay (2016) Aquaporin 4 as a NH3channel. Journal of Biological Chemistry 291:19184-19195

51. P. Wanitchakool, J. Ousingsawat, L. Sirianant, Nanna MacAulay, Rainer Schreiber & Karl Kunzelmann (2016) Cl-channels in apoptosis. European Biophysical Journal 45: 599-610

50. Brian Roland Larsen, Rikke Holm, Bente Vilsen & Nanna MacAulay (2016) Glutamate transporter activity promotes enhanced Na+/K+-ATPase-mediated extracellular K+management during neuronal activity. Journal of Physiology 594:6627-6641      

49. Brian Roland Larsen, Anca Stoica & Nanna MacAulay (2016) Managing brain extracellular K+ during neuronal activity: The physiological role of the Na+/K+-ATPase subunit isoforms. Frontiers of Physiology 7(141):fphys.2016.00141

48. Kasper Lykke, Kathrin Töllner, Peter W. Feit, Thomas Erker, Nanna MacAulay & Wolfgang Löscher (2016)The search for NKCC1-selective drugs: Structure-function relationship of bumetanide and various bumetanide derivatives in inhibiting the human cation-chloride cotransporter NKCC1A. Epilepsy & Behavior 59:42-49

47. Nils Landegren, M. Pourmousa Lindberg, J. Skov, Å. Hallgren, D. Eriksson, Trine Lisberg Toft-Bertelsen, Nanna MacAulay, E. Hagforsen, A. Räisänen-Sokolowski, H. Saha, T. Nilsson, G. Nordmark, S. Ohlsson, J. Gustafsson, E.S. Husebye, E. Larsson, M.S. Anderson, J. Perheentupa, F. Rorsman, Robert A. Fenton & O. Kämpe (2016) Autoantibodies targeting a collecting duct-specific water channel in tubulointerstitial nephritis. Journal of American Society of Nephrology 27:3220-3228

46. Hanne B. Moeller, J. Slengerik-Hansen, T. Aroankins, Mette Assentoft, Nanna MacAulay, Søren K. Moestrup, V. Bhalla & Fenton RA (2016) Regulation of the water channel aquaporin-2 via 14-3-3θ and -ζ. Journal of Biological Chemistry 291:2469-84

2015

45. Andrew Jo, Daniel A. Ryskamp, Tam T.T. Phuong, Alan S. Verkman, Oleg Yarishkin,  Nanna MacAulay & David Križaj (2015). TRPV4 and AQP4 channels synergistically regulate cell volume and calcium homeostasis in retinal Müller glia. Journal of Neuroscience 35:13525-13537

44. Shreyas Kaptan, Mette Assentoft, Hans Peter Schneider, Robert A. Fenton, Joachim Deitmer, Nanna MacAulay & Bert de Groot (2015) Is H95 a pH-dependent gate in aquaporin 4? Structure 23:2309-2318

43. Jette S. Alstrom, Daniel B. Hansen, Morten S. Nielsen & Nanna MacAulay (2015) Isoform-specific phosphorylation-dependent regulation of connexin hemichannels. Journal of Neurophysiology 14:3014-22

42. Annette B. Steffensen, Jeremy Sword, Deborah Croom, Sergei A. Kirov & Nanna MacAulay (2015) Cotransporters as a molecular mechanism underlying spreading depolarization-induced dendritic beading. Journal of Neuroscience 35:12172-12187

41. Kasper Lykke, Mette Assentoft, Robert A. Fenton, Mette M. Rosenkilde & Nanna MacAulay (2015). Vasopressin receptors V1aand V2 are not osmo-sensors. Physiological Reports 3:phy2.12519

40. Kasper Lykke, Kathrin Töllner, Kerstin Römermann, Peter W. Feit, Thomas Erker, Nanna MacAulay & Wolfgang Löscher (2015) Structure-activity relationships of bumetanide derivatives: correlation between diuretic activity in dogs and inhibition of human NKCC2 variant A.British Journal of Pharmacology 172:4469-4480

39. Mette Assentoft, Brian Roland Larsen & Nanna MacAulay (2015) Regulation and function of AQP4 in the central nervous system.Neurochemical Research 40:2615-2627

38. Martin N. Andersen, Lasse Skibsbye, C. Tang, F. Petersen, Nanna MacAulay, Hanne B. Rasmussen & Thomas Jespersen (2015) PKC and AMPK regulation of Kv1.5 potassium channels. Channels 9:121-128

37. Jette S. Alstrom, Line W. Stroemlund, Morten S. Nielsen & Nanna MacAulay (2015) Protein kinase C-dependent regulation of connexin43 gap junctions and hemichannels. Biochemical Society Transaction 43:519-523

36. Daniel Ryskamp, Andrew Jo, Amber Frye, Felix Vazquez-Chona, Nanna MacAulay, Wallace Thoreson & David Krizaj (2015) Swelling and eicosanoid metabolites differentially gate TRPV4 channels in retinal neurons and glia. Journal of Neuroscience 34:15689-15700

2014

35. Brian Roland Larsen & Nanna MacAulay (2014) Kir4.1-mediated spatial buffering of K+: Experimental challenges in determination of its temporal and quantitative contribution to K+clearance in the brain. Channels 8:544-560

34. Mette Assentoft, Brian Roland Larsen, Emma T.B. Olesen, Robert A. Fenton & Nanna MacAulay (2014) AQP4 plasma membrane trafficking or channel gating is not significantly modulated by phosphorylation at C-terminal serine residues. American Journal of Physiology – Cell Physiology 307:957-965

33. Daniel Bloch Hansen, Ye Zu-Cheng, Kirstine Calloe, Thomas H. Braunstein, Johannes P. Hofgaard, Bruce R. Ransom, Morten S. Nielsen & Nanna MacAulay (2014) Activation, permeability, and inhibition of astrocytic and neuronal large pore (hemi)channels. Journal of Biological Chemistry 289:26058-26073

32. Daniel Bloch Hansen, Thomas H. Braunstein, Morten S. Nielsen & Nanna MacAulay (2014) Distinct permeation profiles of the connexin 30 and 43 hemichannels. FEBS Letters588:1446-1457

31. Brian Roland Larsen, Mette Assentoft, Maria L. Cotrina, Susan Z. Hua, Maiken Nedergaard, Kai Kaila, Juha Voipio & Nanna MacAulay (2014)Contributions of the Na+/K+-ATPase, NKCC1, and Kir4.1 to hippocampal K+-clearance and volume responses. GLIA 62:608-622

2013

30. Thomas Zeuthen, Magnus Alsterfjord, Eric Beitz & Nanna MacAulay (2013) Osmotic water transport in aquaporins. Evidence for a stochastic mechanism. Journal of Physiology 591:5017-5029

29. Rikke Sogaard, Lars Borre, Thomas H.  Braunstein, Kenneth L. Madsen & Nanna MacAulay (2013) Functional modulation of the glutamate transporter variant GLT1b by the PDZ domain protein PICK1. Journal of Biological Chemistry 288:20195-20207

28. Mette Assentoft, Shreyas Kaptan, Robert A. Fenton, Susan Z. Hua, Bert L. de Groot & Nanna MacAulay (2013) Phosphorylation of rat aquaporin-4 at Ser111 is not required for channel gating. GLIA 61:1101-1112

2012

27. Lene L. Rosenbaek, Mette Assentoft, Nis B. Pedersen, Nanna MacAulay & Robert A. Fenton (2012) Characterization of a novel phosphorylation site in the sodium chloride transporter, NCC. Journal of Physiology 590:6121-6139

26. Nanna MacAulay & Thomas Zeuthen (2012) Glial K+ clearance and cell swelling: key roles for cotransporters and pumps. Neurochemical Research 37:2299-2309

25. Thomas Zeuthen & Nanna MacAulay (2012) Transport of water against its concentration gradient: fact or fiction? WIREs: Membrane Transport and Signaling 1(4):373-381 (doi:10.1002/wmts:54)

24. Thomas Zeuthen & Nanna MacAulay (2012) Cotransport of water by the Na+-K+-2Cl-cotransporter expressed in Xenopusoocytes: NKCC1 versus NKCC2. Journal of Physiology 590:1139-1154

23. Rikke Søgaard, Ivana Novak & Nanna MacAulay (2012) Differential effect of ammonia on three glutamate transporter isoforms expressed in Xenopusoocytes. American Journal of Physiology – Cell Physiology 302:880-891

2010

22. Robert A. Fenton, Hanne B. Moeller, Marina Zelenina, Marteinn T. Snaebjornsson, Torgeir Holen & Nanna MacAulay (2010) Differential water permeability and regulation of three AQP4 isoforms. Cellular and Molecular Life Sciences 67:829-840

21. Nanna MacAulay & Thomas Zeuthen (2010) Water transport between CNS compartments: contribution of aquaporins versus co-transporters. Neuroscience 168:941-956

2009

20. Hanne B. Moeller, Robert A. Fenton, Thomas Zeuthen & Nanna MacAulay (2009) Vasopressin-dependent short-term regulation of AQP4 expressed in Xenopusoocytes. Neuroscience 164:1674-1684

19. Nanna MacAulay, Steffen Hamann & Thomas Zeuthen (2009) Chloride transporters as water pumps: elements in a new model of epithelial water transport. In: Physiology and pathology of chloride transporters and channels in the nervous system. Eds FJ Alvarez-Leefmans & E Delpire. Academic Press, Elsevier pp 547-568

18. Rikke Soe, Nanna MacAulay & Dan Klaerke (2009) Modulation of Kir4.1 and Kir4.1-Kir5.1 channels by small changes in cell volume. Neuroscience Letters 457:80-84

17. Anne-Kristine Meinild, Don D. Loo, Søren Skovstrup, Ulrik Gether & Nanna MacAulay (2009) Elucidating conformational changes in the γ-aminobutyric acid (GABA)-transporter-1. Journal of Biological Chemistry 284:16226-16235

16. Rikke Søgaard, Magnus Alsterfjord, Nanna MacAulay & Thomas Zeuthen (2009) Ammonium ion transport by the AMT/Rh homolog TaAMT1;1 is stimulated by acidic pH. Pflugers Archives 458:733-743

15. Hanne B Moeller, Nanna MacAulay (shared first author), Mark A Knepper & Robert A Fenton (2009) Role of multiple phosphorylation sites in the carboxyl-terminal tail of aquaporin-2 for water transport: Evidence against channel gating. American Journal of Physiology - Renal Physiology 296:649-657

2007

14. Thomas Zeuthen, Emil Zeuthen & Nanna MacAulay (2007) Water transport by GLUT2 expressed in Xenopus laevis oocytes. Journal of Physiology579:345-361

2005

13. Aidas Alaburda, Raul Russo, Nanna MacAulay & Jørn Hounsgaard (2005) Periodic high and low conductance states in spinal motoneurons and interneurons during scratch-like network activity in adult turtles. Journal of Neuroscience 25:6316-6321

2004

12. Nanna MacAulay, Steffen Hamann & Thomas Zeuthen (2004) Water transport in the brain: role of cotransporters.Neuroscience 129:1031-1044

2003

11. Nanna MacAulay, Anne-Kristine Meinild, Thomas Zeuthen & Ulrik Gether (2003) Residues in the extracellular loop 4 are critical for maintaining the conformational equilibrium of the γ-aminobutyric acid (GABA) transporter-1. Journal of Biological Chemistry 278:28771-28777

10. Morten Grunnet, Thomas Jespersen, Nanna MacAulay, Nanna K. Jørgensen, Nicole Schmitt, Olaf Pongs, Søren-Peter Olesen & Dan Klærke (2003) KCNQ1 channels sense small changes in cell volume.Journal of Physiology 549:419-427

2002

9. Nanna MacAulay, Thomas Zeuthen & Ulrik Gether (2002) Conformational basis for the Li+-induced leak current in the rat γ-aminobutyric acid (GABA) transporter-1. Journal of Physiology 544:447-458

8. Nanna MacAulay, Ulrik Gether, Dan Klærke & Thomas Zeuthen (2002) Passive water and urea permeability of a human Na+/glutamate cotransporter expressed in Xenopus oocytes. Journal of Physiology 542:817-828

7. Morten Grunnet, Nanna MacAulay, Nanna K. Jørgensen, Bo S. Jensen, Søren-Peter Olesen & Dan Klærke (2002) Regulation of cloned Ca2+-activated K+channels by cell volume changes. Pflugers Archives 444:167-177

6. Thomas Zeuthen & Nanna MacAulay (2002) Passive water transport in biological pores. In International Review of Cytology, vol 215, eds T. Zeuthen & W.D. Stein., pp 259-284. Academic Press, San Diego.

5. Thomas Zeuthen & Nanna MacAulay (2002) Cotransporters as molecular water pumps. In International Review of Cytology,vol 215, eds T. Zeuthen & W.D. Stein., pp 203-230. Academic Press, San Diego.

2001

4. Nanna MacAulay, Annie Bendahan, Claus Juul Loland, Thomas Zeuthen, Baruch I. Kanner & Ulrik Gether (2001) Engineered Zn2+ switches in the GABA transporter-1: differential effects on GABA uptake and currents. Journal of Biological Chemistry276:40476-40485

3. Nanna MacAulay, Ulrik Gether, Dan Klærke & Thomas Zeuthen (2001) Water transport by the Na+-coupled glutamate cotransporter expressed in Xenopusoocytes. Journal of Physiology 530:367-378

1998

2. Rhoda Blostein, Stewart E. Daly, Nanna Boxenbaum, Lois K. Lane, José M. Arguello, Jerry B. Lingrel, Steve J. D. Karlish & Michael J. Caplan (1998) Conformational alterations resulting from mutations in cytoplasmic domains of the α-subunit of the Na,K-ATPase. Acta Physiologica Scandinavia (Supplement) 643:275-281

1. Nanna Boxenbaum, Stewart E. Daly, Zahid Z. Javaid, Lois K. Lane & Rhoda Blostein (1998) Changes in steady-state conformational equilibrium resulting from cytoplasmic mutations of the Na,K-ATPase α-subunit. Journal of Biological Chemistry 273:23086-23092

 

 

 

 

Lab members

Name Title Job responsibilities Image
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Beatriche Louise Edelbo PhD Fellow MacAulay Lab Billede af Beatriche Louise Edelbo
Dennis Bo Jensen Postdoc MacAulay Lab Billede af Dennis Bo Jensen
Ida Marchen Egerod Israelsen Postdoc MacAulay Lab Billede af Ida Marchen Egerod Israelsen
Nanna MacAulay Professor MacAulay Lab Billede af Nanna MacAulay
Nina Rostgaard Guest Researcher MacAulay lab Billede af Nina Rostgaard
Thomas Zeuthen Professor Emeritus MacAulay Lab Billede af Thomas Zeuthen
Trine Lind Devantier Laboratory Coordinator MacAulay Lab Billede af Trine Lind Devantier
Trine Lisberg Toft Assistant Professor MacAulay lab Billede af Trine Lisberg Toft